TSH Receptor (TSHR) (NM_000369) Human 3' UTR Clone

CAT#: SC217211

3`UTR clone of thyroid stimulating hormone receptor (TSHR) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TSHR
• Synonyms: CHNG1; hTSHR-I; LGR3
• ACCN: NM_000369
• Insert Size: 1978 bp
• Sequence Data:
  Insert Sequence

  >SC217211 3'UTR clone of NM_000369
  The sequence shown below is from the reference sequence of NM_000369. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAGAAGAGTATATGCAAACGGTTTTGTAAGTTAACACTACACTACTCACAATGGTAGGGGAACTTACAAA
  ATAATAGTTTCTTGAATATGCATTCCAATCCCATGACACCCCCAACACATAGCTGCCCTCACTCTTGTGC
  AGGCGATGTTTCAATGTTTCATGGGGCAAGAGTTTATCTCTGGAGAGTGATTAGTATTAACCTAATCATT
  GCCCCCAAGAAGGAAGTTAGGCTACCAGCATATTTGAATGCCAGGTGAAATCAAAATAATCTACACTATC
  TAGAAGACTTTCTTGATGCCAAGTCCAGAGATGTCATTGTGTAGGATGTTCAGTAAATATTAACTGAGCT
  ATGTCAATATAGAGCTTCTCAGTTTTGTATAACATTTCATACTAAAGATTCAGCAAATGGAAAATGCTAT
  TAATTTGGTTGGTGACCACAAGATAAAATCAGTCCCACGTTGGCTCAGTTCAACTAGATGTTCCCTGATA
  CAAAGAGAACTTGATTTCCTTAAAACTGAAAAGCCAAACACAGCTAGCTGTCATACAAGAAACAGCTATT
  ATGAGACATGAAGGAGGGTAAGAATTAGCTTTAAGTTTTGTTTTGCTTTGTTTTGTTTTTTAACTCAACC
  TATTAATCATCTCTTCACAAGAATCCACCTGATGTGACCAAGCTATTATGTGTTGCCTGGAAAAACTGGC
  AAGATTTCAGCTTATGTGGCCTAGCAAACTAAGAATTGCTCTTCTTGGCCAGCCTCATAGCATAAAAGAT
  GTGAACTCTAGGAAGTCTTTCTGAGTAGCAATAAGTGGGAATTATGGGCAGAGCACACTCAATCCCCTGT
  TGATTAATAAAACAGGCTGGACACTAATTAACTATGGGACTTAAATCTGTAGAAATGAAGGAGTCCAATA
  GCTTCTTCCAATTTTAAAACTCTAGTACATCCCTTTCCCTCAAATATATATTTCTAAGATAAAGAGAAAG
  AAGAGCACTAAGTAAGTAGAATCTGTTTTTCCTATTTTGTAGGGCTGCTGACTCCTAGTCCTTGAAGCCT
  AGACACATGACCCAGGAAATTTTTCCTTTGTTTCACTTTTGATTATGATGTCTGAGCCAAAAATTCAATT
  AAGTAAACATACTCGCCTGGATCTGAATCATTCATTTAATTACTAGATCTACCCAGCTGTTATATCAGGC
  CAAAAACAGATTCGTGTTTATATAAAAGAGTAAACGATGGTTGCAAATTTTGGCTATTTAGAGTTGCTAC
  TTCACTATGAAGAGTCACTTCAAAACACTTCGCTTGTCTTTAGGGATGATTTTTGCCATTTCCAGTCCAC
  GGTATGATACTAAAGCTGTCAAGAGAGGTTTCTTCTTTTCTGAAACTGCCAGCTCTTTCCAGCCCTGTTG
  ATCACTGGACATAAAGCTTCTTTTCCCCAATAATTCTTCTTTACTTAAAATAGTCAGGATCTTTATCTAC
  AGATGTACTCTCCAGGTTACCTGTGATGATAGCCCCCTAATGTCCTGCTAGAAAAGTCTCCAAGCAGAGA
  TGACATTACTTCTGAATGCTCATAAACCACACCATGAAATAAAAGCTCTTTGTTGTTTTAAGATTGTGAA
  GTGTCGTTAATGGGTCCCCACAGATGGTCCCTGCTGGACTCACCTGGAATCTCTCCACAGCCATACCCAC
  TCATCACTATCATTGAGACCTGCACATCTTAATAGAAATATTATAAACATCGAAAATCATGACTTACCTA
  GAAGTTCGCTTGTAACTAATGAAATTAAACAAATGTGTTGCCTTTTGTCATGTGTTTCTCTCCTGTGACA
  TTTCAAAATATCACATCTTGATAAATAATGTGTTTCATCTTGAATAGCTGAACTAATTGCTTTGGAAACA
  GAGTCCTAGAAAAGTGACTTCAACAGAATTGTTACTAAAATTTGCACTCACAACATGAAATAAATTTTCT
  TCCTATGGAATAATCGTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000369.2
• Summary: 'The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]'
• Locus ID: 7253