JAK3 (NM_000215) Human 3' UTR Clone

CAT#: SC217271

3`UTR clone of Janus kinase 3 (JAK3) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: JAK3
• Synonyms: JAK-3; JAK3_HUMAN; JAKL; L-JAK; LJAK
• ACCN: NM_000215
• Insert Size: 1963 bp
• Sequence Data:
  Insert Sequence

  >SC217271 3'UTR clone of NM_000215
  The sequence shown below is from the reference sequence of NM_000215. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AACACCACTCCCTGTCCTTTTCATAGCTCCTGCCCGCAGACCTCTGGATTAGGTCTCTGTTGACTGGCTG
  TGTGACCTTAGGCCCGGAGCTGCCCCTCTCTGGGCCTCAGAGGCCTTATGAGGGTCCTCTACTTCAGGAA
  CACCCCCATGACATTGCATTTGGGGGGGCTCCCGTGGCCTGTAGAATAGCCTGTGGCCTTTGCAATTTGT
  TAAGGTTCAAGACAGATGGGCATATGTGTCAGTGGGGCTCTCTGAGTCCTGGCCCAAAGAAGCAAGGAAC
  CAAATTTAAGACTCTCGCATCTTCCCAACCCCTTAAGCCCTGGCCCCCTGAGTTTCCTTTTCTGTCTCTC
  TCTTTTTATTTTTTTTATTTTTATTTTTATTTTTGAGACAGAGCCTCGCTCTGTTACCCAGGGTGGAGTG
  CAGTGGTGCGATCTCGGCTCAGTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTCCTGCCTCAGCCTCC
  CGAGTAGCTGGGATTACAGGTGTGCACCACCACACCCGGCTAATTTTTTTTATTTTTAATAGAGATGAGG
  TTTCACCATGATGGCCAGGCTGATCTCGAACTCCTAACCTCAAGTGATCCTCCCACCTCAGCCTCCCAAA
  GTGTTGGAATAATAGGCATGAGCCACTGCACCCAGGCTTTTTTTTTTTTAAATTTATTATTATTATTTTT
  AAGAGACAGGATCTTGCTACGTTGCCCAGGCTGGTCTTGAACTCCTGGGCTACAGTGATCCTCCTGCCTT
  ATCCTCCTAAATAGCTGGGACTACAGCACCTAGTTTTGAGTTTCCTGTCTTATTTCCAATGGGGACATTC
  ATGTAGCTTTTTTTTTTTTTTTTTTTTTGAGACGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTACAGTGG
  CGCAATCTAGGCTCACTGCAAGCTCCGCCTCCTGGGTTCACACCATTCTCTCGCCTCAGCCTCCCAAGTA
  GCTGGGACTACAGGCGCCCGCCACCACACCCGGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCAC
  CTTGTTAGCCAGGATGGTTTCCATCTCCTGACCTCGTGATCTGCCCGTCTCGGCCTCCCAAAGTGCTGGG
  ATTACAGGCATGAGCCACTGCGCCCGGCCCTCATGTAGCTTTAAATGTATGATCTGACTTCTGCTCCCCG
  ATCTCTGTTTCTCTGGAGGAAGCCAAGGACAAGAGCAGTTGCTGTGGCTGGGACTCTGCCTTTTAGGGGA
  GCCCGTGTATCTCTTTGGGATCCTGAAAGGGGGCAGGAAAGGCTGGGGTCCCAGTCCACCCTAATGGTAT
  CTGAGTGTCCTAGGGCTTCAGTTTTCCCACCTGTCCAATGGGACCCTTTCTGTCCTCACCCTACAAGGGG
  CACAAAGGGATGACACCAAACCTGGCAGGAACTTTTCACGCAATCAAGGGAAGGAAAGGCATTCCTGGCA
  GAGGGAACAGCATGCCAAGCGTGAGAAGGCTCAGAGTAAGGAGGTTAAGAGCCCAAGTATTGGAGCCTAC
  AGTTTTGCCCCTTCCATGCAGTGTGACAGTGGGCAAGTTCCTTTCCCTCTCTGGGTCTCAGTTCTGTCCC
  CTGCAAAATGGTCAGAGCTTACCCCTTGGCTGTGCAGGGTCAACTTTCTGACTGGTGAGAGGGATTCTCA
  TGCAGGTTAAGCTTCTGCTGCTCCTCCTCACCTGCAAAGCTTTTCTGCCACTTTTGCCTCCTTGGAAAAC
  TCTTATCCATCTCTCAAAACTCCAGCTACCACATCCTTGCAGCCTTCCCTCATATACCCCCACTACTACT
  GTAGCCCTGTCCTTCCCTCCAGCCCCACTCTGGCCCTGGGGCTGGGGAAGTGTCTGTGTCCAGCTGTCTC
  CCCTGACCTCAGGGTTCCTTGGGGGCTGGGCTGAGGCCTCAGTACAGAGGGGGCTCTGGAAATGTTTGTT
  GAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000215.3
• Summary: 'The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq, Jul 2008]'
• Locus ID: 3718