SSH3BP1 (ABI1) (NM_001012751) Human 3' UTR Clone

CAT#: SC217272

3`UTR clone of abl-interactor 1 (ABI1) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ABI1
• Synonyms: ABI-1; ABLBP4; E3B1; NAP1BP; SSH3BP; SSH3BP1
• ACCN: NM_001012751
• Insert Size: 1992 bp
• Sequence Data:
  Insert Sequence

  >SC217272 3'UTR clone of NM_001012751
  The sequence shown below is from the reference sequence of NM_001012751. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCTGGGAACTATGTTGAATCAATCATGCACTATACTGATTAATTTTTTTTTTTCTTTTGAAGTAGATTCT
  TATTACTCAGTCATACTGTGGGACTATTATGGTTAACAGAACTGTCTTAATATGTTTTAAAATGTGCCCA
  TATTTTCAGAACATGCTGTTTTATTGGTAAATTGAATGTCTACCTGTAAGCATAAATCTTTGAGGCAGTT
  TATGTATTGCTGAATAGCAATTTATACAAGAAGCTGTCCATAACTGATTATGCTTATGTACTTACTTACA
  CATTTTTAACTTTATGACCAGCCTAAATATTCTGGGGGAAGTGGGGTATAATATTTAACGAATCATGATT
  CAGATTGTACCATTACATGTTTCAGTGCAGCATGGTTACTAACGCTATGTCAGACTAATATTAAAATCAG
  AAAATTTAAATGCTGGTGCTGGTCAGACTTTTTTTGTTAGATTCTCTCATTTAAAAAAAATACTGTTTGT
  TTAAAGCATGCATAAAAATTTATGTATTGAAATATACTTAAAAATTCAAGATGCTTCCCATTTGTGTAAT
  ATTTACCTGGAGGACTCGTACTTAGGTGTCTTAACGTGAATTGAGTCTCCAAGGTCTCCATGTGAAACAA
  GCAAAAAGAGAATTATCTGTAATGTTGTAATTTGTACCTAAGTTTTTTAATGAGTGAAATTTGCATTATA
  AACTTTTTCCATTCATAAATACATAAGTGAACCAAAGGTTTTTGTCCTTTCCTTCACTGATTTGCTTTAA
  AAAAAATAAAAGATAATGATTTATTGCAGAATTATGATTCTATTTTCTCAATATGTTAACTTGGAAAAAA
  ATTTTAGCCTTATCTTAATCTGTCCCAACAGCAATGTGACGGATTTTTGCAGATTCAAAATCTGCAATGG
  TTATTTACAAGTCAATCTACTGAATTCCTTTTTTAAATAATCTTTTGAAACTAAGAAAATGTGTCAAATT
  GTGTGCATTCATTCTGTAGGTAAAATTCTTAAGGATTGCCATGTCAGTCCTTCAGTTGTACAGTGAACTG
  TTGTACACTGGTTCAATGAAAGGAAGTTAAAAGTACCTTTTACATATTGTAAAAGTGGATACAGTTGATT
  TGTGAGTAGGCACTCTTTAATCCATTACCTGGCACTAGCAACATTAGAATTTTAAAATAAAATAATTGGG
  AAAGAAGGTGGGTCATGTATTAATCAGTGAACAGAGATTTACCTAACCAACAGACTTGGATTGTCTTTTG
  ACATAATCAAAATGCAACACATGCACTTTGTGTGTCTCCTCTTAATTGAAGGGAGGGCTGAGGGATGTTT
  TCTCTTCTTGTCTTGTGTATAATTCTCTATTGCTTAGGATATTAAAGTAGAGCACTCAAGTGTGGGTTTC
  TGTGTTATTGAGGATTTGTTTGGAATTCAAATTACAGTTATGTACTGGATGCTACAGACTTATAACAGCA
  TAGTGAATGGTAAGACTAGTGCAAAACAGTTATTTCTGAAAATTAAAGACCATTATTGCTACCAAATCAA
  TGTGACTATTTCATATGCATTTTGCCTTTGTTAATTTTAAACAAACAAAGTATCATTAGTGATCAGCTAG
  CTACCTTCTACTTTCCATTTTTCAAGTGGATTGTTCTCTTAATTTGTATATAACCTGTTGTCTAAATTTT
  ATGTACAGTCTTTTATAATAAACCATTCTCCTATATGAAATTTTGGATACTGTTAAAATATAACTCATGT
  GGACTTCAATGCAGACTTCACATTTTTCTGACCTTTACTCCTACGGTAAATCAAGACAAACCATTTGAAT
  TTTAACATTGGTTATTTATTTGTGTAATGGCCTTACATGTGGATATTAACAGTGTAAACAATACCACATA
  TAACCATCAAGATACCTTGATTATAATTTATAGGACTAGAATGACAGATTCAGGTGGTTTATTAATTACA
  TTTAAAAAGTTTTCTGAAAAAACTTTTGCAAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001012751.1
• Summary: This gene encodes a member of the Abelson-interactor family of adaptor proteins. These proteins facilitate signal transduction as components of several multiprotein complexes, and regulate actin polymerization and cytoskeletal remodeling through interactions with Abelson tyrosine kinases. The encoded protein plays a role in macropinocytosis as a component of the WAVE2 complex, and also forms a complex with EPS8 and SOS1 that mediates signal transduction from Ras to Rac. This gene may play a role in the progression of several malignancies including melanoma, colon cancer and breast cancer, and a t(10;11) chromosomal translocation involving this gene and the MLL gene has been associated with acute myeloid leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 14. [provided by RefSeq, Sep 2011]
• Locus ID: 10006