TAF1 (NM_138923) Human 3' UTR Clone

CAT#: SC217282

3`UTR clone of TAF1 RNA polymerase II TATA box binding protein (TBP)-associated factor 250kDa (TAF1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TAF1
• Synonyms: BA2R; CCG1; CCGS; DYT3; DYT3/TAF1; KAT4; MRXS33; N-TAF1; NSCL2; OF; P250; TAF(II)250; TAF2A; TAFII-250; TAFII250; XDP
• ACCN: NM_138923
• Insert Size: 1968 bp
• Sequence Data:
  Insert Sequence

  >SC217282 3'UTR clone of NM_138923
  The sequence shown below is from the reference sequence of NM_138923. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GACAGTGACTTGGACTCTGATGAATGAGGCTTCCTTTGGGCCTCCTTGGTCAGCCTTCCCTGTTCTCCAG
  CCTAGGTGGTTCACCTTTCCCCAATTTGTTCATATTTGTACAGTATCTGATCCTGAAATCATGAAATTAA
  CTAACACCTTAGCCTTTTTAAAAGTAGTAAGTAAATGATAATAAATCACCTCTCCTAATCTTCCTGGGGC
  AATGTCACCCTTTGATTTAAAACAAAGCAACCCCCTTTCCCCTACCACTACGGAAAAGAGCAAGCTCATT
  TTTCCGTGTCCTCCTTTATTTAACTCCATTTATTGCTTTTGGTATAATTTTTCCCTGGGGAAGGAGGGGA
  AATTATGAAAGAACTAGTAACTTTATGTCCTCTTGATGTATTAGGAAATTTCCGGCCAGGCGTGGTGGCT
  CACACCTGTAATCTCAGCACTCTGGGAGGCCGAGGCGGGCAGATCACCTGAGGTCAGAAGTTCGAGACCA
  GCTTGGCCAACATGGCGAAACCGCATCTCTACTAAAAATACAAAAATTAGCCAGGTGTGGTGGCGTATGC
  CTGTTAATCCTAGCTACTCGGGAGGCTGAGGCAGGAGAATTACTTGAACCCGGGAGGCAGAGGTTGCAGT
  GAGTGGAGGTCACGCCACTGCACTCCAGCCTGGGCGAAAGAGTGAGATTCAGTCTCAAAAAAAAAAAAAT
  TTCCAAGCATGGTATCATCTCACTTTTCTAATTTACAGGCTGGAGCAGATGAGAGCCCTCCTGCTGGGAC
  AGAGAATTGGGTTCTAGTGGACTCTGTGCTACACTTAAACCTGTGAGACAAACCGCCCATTATTTTATTA
  TTTAATTATGCAATGCCTAGTTCCTAAATGGATTGGAGGCAAATTACCGTAAATTTTGAAACAGCCTATA
  TGTCAGAAATGATAATGTTGCCACCTAAATGTTTTCTGTCCCCCCCACCCTCCCCAGGGGAAATGGTAGG
  AAAATGGTAAGTTTCTTAGGGCAAAGACTGTGTCTTCTGTTTCTTTTCATGCTTAGGATATGGTTCTGTG
  CATAGTAGGTACTCAGTAAATGTTCCTAGAATCATAAAATCCTCAACAGATATGTTACTGAGCATCTGCT
  TTTCATGATAAGCACTCTATCAGATCCTTGGGATGCAAAGGTAAATAAGACAAATCCCTTTTACCCAAAG
  AGCTCACCATCAAGTTGGGGGAGGGAAAGTGGAATTCAAAACATGTTAATAAATCATCATAGTACTGTGA
  GATAAGTGCAATTAAGAAGCTAGTTATAAAGTATAGGGGAAATAGAGGAGTAATCATGTCTGAAAAGTCA
  GGAAAGTCTTCCTAGAGGTAATTTTTAAGCTGATTGTTTTAGAATTAGTAGAAGCTTGCCAGATGGAAAA
  GTCCAGGCAAAGTGTAACATGAATGGGAAAGGCCACAGTCTAGAAATGGCAGAGTGTGTTCCTAGTTTGT
  TTGTTTGTTTGTTTGTACCTGCCTTGTTCCAGGAAGGATTTAATGTGGTTTATATTCCAGTCCTTTAATG
  CTGGAAGGGCTGAGATGAGACTGAAAGATGGGCAGGAAGTATATCATCACAAGCTTTGTGTTTGATGTTA
  ATGTGTATGATTTTTATATTATGGGAAATAAGCTCTTAGAGGAGTGATATAATCAGGTTTGTGTTTTAGA
  AATCTGTGTAATGAATGAATGAAGAAAGAAATTGAAGAATCATGTAACATATGTGATCGCATTTTTGTAA
  AAGAACCATGTGTGTTTATATGTGTTTATATATATACTTGTGTATGCAAAGGTAAAAGTCTGAAAGGATA
  TATGCTAACTGTTCACAATGATAACCCCCCAGGAATGGGATTGGAGGGGAGGGGGCTTCTGTGTTTGTTA
  TGTATGCTGGGTGGGATATTGTGCTTTTATTTCTATATTGTTTGAATTTTTTTACAGTATGTATTATTTT
  TGTAATAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_138923.2
• Summary: 'Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons. [provided by RefSeq, Oct 2013]'
• Locus ID: 6872