WDR9 (BRWD1) (NM_001007246) Human 3' UTR Clone

CAT#: SC217303

3`UTR clone of bromodomain and WD repeat domain containing 1 (BRWD1) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: BRWD1
• Synonyms: C21orf107; DCAF19; N143; WDR9; WRD9
• ACCN: NM_001007246
• Insert Size: 2015 bp
• Sequence Data:
  Insert Sequence

  >SC217303 3'UTR clone of NM_001007246
  The sequence shown below is from the reference sequence of NM_001007246. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAGGAAGGCAGTCTTTGCTACGTACAGCAAAAGGTACCTTAATTTGAAGAAGTGTTCTGCTTTGTATTAA
  TAGCCTAATAATTATTATAATTGTGAAGTAATGTAAGCATTGTGGAAGAAAGGTGATAATGTAACTATAA
  ATCATGCTGTGGACTATAAAATTCTCTGCATTTAGCATAGAACATTGAATTTTATTACATAAGTGTTTAA
  TGGGTCCTTCTGCATTAAAAAGTTGATAAAGATATAGATGTTAAGCTAAAAAGCTTGAATTTATTCTGAG
  ATGTTGTTTGAGGTTTTATCAATGTGAGTTCTTAATTTTCCATTTTGTAGCTCACTCATAACTTCATTGA
  ATGTAATTGTATGTGTCTAATAAACTCTTTAAATGCTAGAGGTATTTCATTTGATATTTAGCTTCTGAAA
  GCCCTTTGTTATAAAGGGAAGTGATTTTCTAAATTTGGTAGCAGCTTGAAAACAAGCACCACAAATGTAA
  AATTGCATGGTTCTTTTTTTACTAATTTAATTTTTTTGCCAAGTTATGTGCTAGCAAAATGTTTGAAATT
  GCTATTCTTTTTCTGTTAGCTTGGTATATATGACTTAGACTAAGTATATGTATGTGATATAATATATGTG
  TACATGTGGCTAAACTGCTAATGGGCAAAGATTGTTGGTTCTTCTGATGCTGAGTATACATCAAGTTCAT
  CCCATGATTATTTTTTATTACGAGCTATTGCTGATGTGATGCTACTCTTAGCTGCACCACCAGTATAAAG
  CTTAACTGAGAAAGAACACCCCTCCCTCTTAGTTCCGTGCCAGACTGGTTTGTTAGCTTTAGTTGTTTCT
  TAGTGCTCTAGCCATGTCACCCTGTGGCACTAATCAGGACACTGTTTATATTCTTCCTCTTCTGTGTCCC
  TATTCACTTTGTAATTATTCTATTTTAGCTGGTTATATGGTAGTTAATTGGTAGCCTGTTTTCCTTTGTT
  TTCTTTACTTGTGTCAAGCTCCATGTCGTTGTTTTGTGATGAGCAGTGATTTAGAACTGGCCAGACCTTA
  GAAGAGTCTTCAGCAAGCTGCCCTAGGGTAGGGGCGTATTCTCTGTTTGTTTTGTACTGGTACCCCAGGA
  GGATGCCTTGTAACTGCTCTTTGGTCATGATAGTGGTAATGAATCCATTCATGTAAAATGCAATATGTCT
  GTTAGTTTGGGGCACCCGAGGCTCAGTAGAGGTTTAGTATGATGCCTTCCCTTTCTTCTTTGAGTTATGA
  AGAAACTAATTTCTGGCACAGTGTGACTGCTTCAAACCTATGTTCTGCTGCTATCTCCTATAGAGGAGGG
  CAGACTTTTTTTTCTTAGTGCATTTGCTGGTAGGTCAAGGAGGGATGGACTTTGAATTAGTGTTCAGAGT
  CACAGATCCTACGTATGTGCTCTTCAGTAGAGGATTTTCTGTGATCCTACAATGAAGGGAAAGCTATATA
  ATTTATTGGTACTTCCTAAAGATAGAGACTAAAGTCATGGTAGTATTGGCCAAATTAATTTTAAAACTGA
  ACAAGTCATGAATTTTCATATAAATCAAAGAACTTTTTCCTTATAAAAGCTTCCATTTTTGAAAAGTTGG
  ATCAGTTGTAAGACCTTTCATCTTTAGTATTGTATGATATAATTCTACATTGAAAAATAAACACTTGGCA
  TTAAATGGGACTTGGAGTCAAAAGATCTGGTTTTATGGTCCATCTTTTACCAATCATTTGCTGTGGATCT
  CTTTAAAGGTCCTGGAACCTTTGAGCCTCAGTTACCTCACTTGTAAATTGGCAAAAATACCTACCTCACA
  GGGTTGTTGTGAGGATTCAATGAGCTAATGTATGTTGAAAGCACTTTGTGAATTGTAAAGTGCTACACAA
  ATGTTAGTTGTTGTTGCTGCTGTATCTGAGATGTCTTAATTTTCTTTTTCTTCAGAAATAGTCTAATAAA
  CCTCCAGGGCTTTTCTGTTAAAGTATACTAGTTGGTAAAATGTTGAATAAACAGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001007246.1
• Summary: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD) residues which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 2 bromodomains and multiple WD repeats. This gene is located within the Down syndrome region-2 on chromosome 21. Alternative splicing of this gene generates multiple transcript variants encoding distinct isoforms. In mouse, this gene encodes a nuclear protein that has a polyglutamine-containing region that functions as a transcriptional activation domain which may regulate chromatin remodelling and associates with a component of the SWI/SNF chromatin remodelling complex. [provided by RefSeq, Jun 2011]
• Locus ID: 54014