GLI2 (NM_005270) Human 3' UTR Clone

CAT#: SC217310

3`UTR clone of GLI family zinc finger 2 (GLI2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GLI2
• Synonyms: CJS; HPE9; PHS2; THP1; THP2
• ACCN: NM_005270
• Insert Size: 1988 bp
• Sequence Data:
  Insert Sequence

  >SC217310 3'UTR clone of NM_005270
  The sequence shown below is from the reference sequence of NM_005270. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGAGAGCAAGTTCCTGAACATGATGACCTAGAGGCCCGAGCGCCTGGTGCTGAGTGCACCCGGAGGGGTC
  ATCGCTGCCCAGAGCCTGGGGATTCCAGCTGTCTTGTCTTTTTCCAAAAAAGTGTTAAATAGGCTTGAGG
  GGTTGTTGCGCAATGGCCGCTTCAGATGACAGATGTTGTAAGAGAAGGTTTATGGGCATCCTCTCTGGTC
  TTTTGGATTATTCCTCAGAACAATGAAAAAAGTCTCCATAGGACAGGAAGGAATGCAAAACTCATTTACA
  CAGTGCTTTCCAGCCTTTGGTGCTTACAGGACCGCGCTGTTCCGGCTTCTTCACGGCTGACATTCGGCTA
  ACGAGGGATTACTTTGGCCAAAACCTTTCAAAGGATATGCAGAAAGATGGTAGGGAGCATTTGGGTTTGA
  ATCTGAATGCTATACTGGATACTCTGCTCCGGAAAGATGAGCTTTTTATTCTACTACTTGGAAGGAAAAG
  GAATTCCTGGTCCACCTGAATTCCTCTATGAAGCCTAACTCTTGAGGTCTCTAACATACCTTGTCATAGA
  GGAAAAGCACAGATTATACCTGGATGATTCAGGAGCACATTCTGATTCCAGGTTTGGTAGAGCTGGCTCT
  TCTACTCCGTAAAGCCGAGTCTGGGACTGGCAGCCCATCCAAGTGTATATGAATGAATAAAGCATCCAAG
  TATATATGAATGAATAAAGTATGTAAGTATCACCAGAAAAAGGAAAGAAAAAATGTACTCCTTGGGGCAA
  GCCCAGAAGCTGCCCTGGCCTCTCCAGACCGTGTTTACAGTGTTTGCATGTAGAATGTAGCCCTTCCTGA
  AAAGAAGACTTGTTTCTAAATACCTCGGGGCTGCTGGAGCCGCTGTGGGTTAGGGATGGACTGAGGCCTC
  GAGGAGTGAGGGTGCACCCGGGGCCCAGCCTCAGGCTGCCCTAGGGATCTCTCAGTAGGAAGAGGAAGTT
  GCGTGTTTACCCAATCCTGTTTCTCCAATGCAACGTCCACCCACTTTACCACCAAAAACTCCAGGGCCTG
  ACGGCAGCCCGGTCCCCCAGCACTCACCAGCAGCCCAGTGTTCTCCACCAAGCCACAGTGTGCATGCCTG
  GTATCCTCCGGATTCCCTTCCTTCTGCCCGCTGAGTCACTGGGCAGAGAATGATGACATGTGTAGGTGGT
  GTGGTTGGGGGTGGAAAGGGGAAGGGGTTGATCCTCAGGACTCTGAGGGAGCATCGTTGAATTTTCCTGT
  TCAGTGTGACCAAGACCCACCTGGAAATGGAATTTGGAACTGGCTTCAGGAGACATCATTCCTGAACACA
  CTGTAGGGTGAATTGGTGCATCTTCCCCACCATACACACACACACACACACACACACACACACACACACA
  CACACACCCCAAACCTTTTCATGGGGAATGTGTGGCAACCTTGCCAAACAGCACCACTCAGAGTGTGACT
  CTGACTGTGACCTTGGCCTTAATGAGGAACTTCTTAGGAGAGTTTGAGGACAAGGCCAACATCGTCATCT
  GGGCTCGCTGCGTCCCAGCACATCAAACTCTGTCCAGAGACAAGGCCAACTGCAAATGAAAGCCAGGGAA
  CATTGCTAAGGGTCTGTGGCTCTGTGGTGGTGTTCATCGCCTTCCTGAGATAGGATTTCCCTTGCCAGTC
  CCAACCTGTATATATTCTGTACAGAAGACATCCCTGAATATACTGTAGGTGAGTCGTCCAGCCAAATTTA
  TATCTCCAAAACATTTTTAGCTTTTTCTACATGCTATGAATTGAGATGACATGCTCAACTTGTAAATAAG
  TCTTTTTGTACATTAAAAAAGTAATTTTTTCATAATTTATCTTGTCTATCTGCTTCCCCCTTGACAGTAG
  TTAATGAGAACCTGGGCAGTAAATTTGGTGCATTCGAGCAGAAATTAGGCTGTATTTTTTCTTAACAGTG
  TCAAAATTGACTATCCCGCCTTTGCCAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005270.4
• Summary: 'This gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008]'
• Locus ID: 2736