PUS10 (NM_144709) Human 3' UTR Clone

CAT#: SC217317

3`UTR clone of pseudouridylate synthase 10 (PUS10) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PUS10
• Synonyms: CCDC139; DOBI
• ACCN: NM_144709
• Insert Size: 1986
• Sequence Data:
  Insert Sequence

  >SC217317 3'UTR clone of NM_144709
  The sequence shown below is from the reference sequence of NM_144709. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CACCTGCTCTGGATGACTAGCTTTCAAATTTGGAGACAAAGAGTATGGTTTTCCTGGCATGATGTGGACA
  TCCATGGAGCACATGCCGTAAAATGGCTGTTTACCCACCATAACGGTGTCTTGAAAACTATTTGGATCAT
  GTTGATCTATATAATTGTTAATTTGTTGTAACATCTCAGGATCTATATATGTGTATATTTTGTGTTAAAT
  TGTTCCAAGGATGTCTTAGGATTTTTCTCATTCCCTCTTTCACCCCCACAAACCAAACTATGAATAATGA
  AATAATTCTCCTTAATTCTTTCATTTAGAGAGGTGCACAAACAGGACACATTCTCTGTTAACCTAAGAAG
  CTGTAATTTCAGCAAGATTTCCCTCCACAAGAGATATACCACCTTTAAAATCATGTTCTAATTTTTGTAA
  ATTATCTGAATAAAAGTTATATCTAGATTTATGCAGCATAATATCCTATAATAGTCTAAGATTGCAAATG
  CAAATCTGACATCATTATTTTCCCCAGGAAGCCTCAAACAAGCTATTACCAATGAAGTTCTTTTGGCTGT
  GATTTGTCATAAATCTTAACCAAACTATGAATAATGAAATAATTCTCATTAATACTTTCATTAAGAAAGA
  ATTTTGACAATCTAAAATTGTCAATATAGGTTTCCTGTGGAGGGAAAGTAAAACCGTAGGAGCCAAGGCA
  TGCCATGGTAGGAGCCTTTCATAAATCTTTTAAATAAGGAGAAACAAGTGCCTTTAATAACTTCAATCCC
  AGGGTTAAGAATTTGAAAAATTGAGATTGCTAAACAAAGACGAACTGGTATTATTTCAGAATGCTTCAAT
  ATATTATCCAATTATAAACATTTTAATAATACCTTATTAAAGTAGATAGGATATTGTCTCCATTTTATAA
  ATATGGAAACCAAAGACTAGAAGTTAAATGACTTATCCAAAATTGTTAGCTACTTCTATATTGAGGACTA
  GAACTGAAGTTTCTTGGTTTCTAGGGTCTTTCTAATAAGCCCTATTACTGAGCACCTGTTTGTGTGAAGG
  GGCAGCTATGGATGCCATTTAAACTCATGTAACTGATAATTAACTTTTGGAATGTAGTTACCAAATCAAA
  GAGCTTACCAAAAAATAGCCCAAGACTATTTAATACAATCTTATATAGTTACCATTTGTTATTAAGTTAA
  ACCTCAGTTGGTATTATGAAGAGGAAAAACATTAAGCTAAACCTAAAAAAAATGAATGGGTCATTTAAGT
  TTCAGAAAGGAGCCTCATTACAATTTCAGATTTGAGTATAATTTCCATCACCCTAATTCAACTATTTGAG
  CTTTTTTTAAGAATACATGTGACTTGACTAGTATTAGATTACTAACCATTTCCCATCTCTATATTAAACA
  TAAATCACAGATACCAAGATTCACTTTAGATATGAGGAAAATGGTTTTAATGGACACAAAGGAGTCAGCC
  ACGTTGGAACCAACATAGTTTCATACCACGTTGAAACCATGTGTTTGATATGCAAATAACAGCAAATAAT
  TTTTTCACTCTTGTCAATGCCAATGCATTGAAAGGCCCAGAAAATGAGAAAAGGATAACAAACTTTTGAT
  AAAAAGGTAAGAATTTCTGTGTGGATGTTCCTCAGTTTCTGGGAAAATTTTGCTACAAAAGAGATTTAAA
  TAATTTAGGAGGCAGTCTTTTGCATGGTATTTTTGGCTTACCTTTGAATCTAGTGGACCTGTAATATGTA
  AGAATGAAGAATGTGCTTAGCAAACTGAAATGAGAAGATTTCTATTTCTTAAATTTCTATTCCTGAGATT
  ATATAAAATTTCACGCAAAGAGTAAGCATTAGCTTTAAATACTTCTTCCAAATGTTCCTAGAATTTTGAG
  ACAAAGATTGTTAAAAATTAATGTATGTCTTTGTTTTAGGCAGAACTGGAAATATTAAGTAGTGAGAGTA
  CTATAGTTTCCCTTTTGCCTACATAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_144709.2
• Summary: Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]). [supplied by OMIM, May 2009]
• Locus ID: 150962