MEGF8 (NM_001410) Human 3' UTR Clone

CAT#: SC217321

3`UTR clone of multiple EGF-like-domains 8 (MEGF8) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MEGF8
• Synonyms: C19orf49; CRPT2; EGFL4; SBP1
• ACCN: NM_001410
• Insert Size: 2004 bp
• Sequence Data:
  Insert Sequence

  >SC217321 3'UTR clone of NM_001410
  The sequence shown below is from the reference sequence of NM_001410. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GACTGTTGAGCCAGGACAACCTCACCAGCATGTCCCTCTGACATGCCCAGGGTTCTCATCCACAGCAGCT
  GGGTCACCTGATAGGGCCGCCCTGGACTTGGGGTCCCTCCACCTGGGGGCCCCTGGACACTGTCTACTTG
  GAGACCACTGGCCCCCTTCCCCCAGGGTTGCCCAGATGGGGCCTCCTTTGTTCTGCATTCAGCAGCTATT
  TATCGAGTACCTACTCTGTCAGGCACTGTCATAGGCGTGGGGCAAAGCAGGAACCAAGAGACGAGGTTCC
  CTGATCTCATGGGACTTAGGTTCTGGTGAAGGGAGACAATCAGTGCACATGCACACACCCCACACGCATA
  CACACATGAACACATGCACATGTGCACACACAAGTAAGATGGCTTCAGAGAGGGAGAAGCACTGTGAGGC
  CTCCAGAGGATGTGGCAGTGAGGGACGATGGGGTGAAGTCAGCTGGGCATTCAAAGAAGCTAGACTGAGA
  ACGCCTGAGAAGAACCAGCTACGGGAAGAGCTTTGGGAAGCAAAGGCAGAGGCCCTGGGGTGGGAGCAGG
  CTTGTTTTATTGGAAGGACCAGAAAACTGGTAAGTGTGACCCAGATCAAGTGTGAGGAGATGAGGCTGGG
  GATAGTCAGGGGCTGGATCACCCAGGGCCTTGTGGGCCCCACATAGGGTTTTGGGTTTTATTCTCAGGGC
  AATGGGAAGCTGTTGGATGGTTTGATGAAGGGGAGTGACAGGATCCGATGTACCTATTTAAGAATTTAAG
  AGGGTCGGGTGCGGTGGCTCATGCCTATAATCCTAGCACTTTGAGAGGCCAAGGTGGGCTGATCACAAGG
  TCAAGAGTTCGTGACCAGCCTGGCCAATATGGTGAAACCCCATGTCTACTAAAAATACAAAAATTAGCTG
  GGCATGGTGGCACACACTTGTAGTCCGAGCTACTCGGGAGGCTGAGGCAGAAGAATCGCTTGAACCCAGG
  AGGCGGAGGTTGCAGTGAGCTCAGATTGCGTCACTGCACTCCAACCTGGGTGACAGAGCGAGACTCCACC
  TCAAAAAAAAAAAAAAAATTTAAGAGGTCACTCAGTTGTGCTGTGGAGAATGGACCGGAGGGACAAGAGG
  GGCAGCAGGGATGGTGGGCTGGAGTAGGGTGCTGGCAATGAGGGAGTCTGGCTCAGATGTGGGATGTGTA
  TGGAAGAATATAAATGATGGTGTGGATGTCAGGGTGAGGGAGGAGACAAAACCACGATGACCCCTAGCTT
  TGTGGCCTGAACTGTGGGTGGCTGAGGGGATCGTTAATTGAATGGGGCAGACTGAGGCTTGTGAGGAAGA
  TCAGAGTCTGGTTCTTGACATGAGATGCCCTTCAGACATCTCTTCACTCAGGTCCAACTAGGGATACAGA
  AACACTGAATATTTCAACAGCAGAAATTGAATGGGGGGATTGATAGCGCTGGCGAGGGAAGCAGCTGGAA
  AGAGACAGATGGCACCCTGAGACAGCCCAGAGGTGAATAGGACCCCCAGGCTGCAGGGATAAAGCTCAGT
  GGTGGTGTTACCTCACCGGGGACCAGGGTCACACAGCAAAGCTGGAACAACAGAGGCGTGTTGTGGGGGA
  GCCTCAGAGGGGACAAAACCTCTGCCTGAGATCCCACCCCAGGTGGGCATGGGGGCCACTGAGGTTGGGG
  ATGAAAATGCCGGTACCGTCAGTGCACAGCCCTGTTCCAGACAGTGCTGCCTGGAAGATTTCTGGGCTCT
  CCTGAGGCGCCACCCCGCACCTGAGCCACCTCCTTGGACTCCTGTCCTCTACCCCTTGAGGACCTCCCTC
  CCTTCTACCCTAGCTGTCTTCTTGAACTTGGGACTCTCCTTTCCCAAGACTTCCATCACTAGCTCCTGGA
  GGGACTGGACTTTGCATCTTCCCTTCGCGTGGAGCCTCAGTGTGAGAGGCCCTAGCCAATGCGTGCATGT
  CAGAGGTGGTGGGGACCACATCAGAAGAAGAGGGGGGTGATGAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001410.2
• Summary: 'The protein encoded by this gene is a single-pass type I membrane protein of unknown function that contains several EGF-like domains, Kelch repeats, and PSI domains. Defects in this gene are a cause of Carpenter syndrome 2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]'
• Locus ID: 1954