p114RhoGEF (ARHGEF18) (NM_015318) Human 3' UTR Clone

CAT#: SC217328

3`UTR clone of Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ARHGEF18
• Synonyms: P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF
• ACCN: NM_015318
• Insert Size: 2004
• Sequence Data:
  Insert Sequence

  >SC217328 3'UTR clone of NM_015318
  The sequence shown below is from the reference sequence of NM_015318. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AAGGAGGACGCCAGCAAAGAAGACGTCATCTTCTTCTAAAAGGGCCGTGACTCAAGGAAAGTTTTTAATG
  GAAAGTTGAGCCAGAACTAAACCAGGGAGCTGTCTGAAATCATAGCACCCCATCCGGGTGGCGGGGAGAT
  CAACTCCGAGCTGTTTTTCCGAGGCAGTGAGGAACGGTGCCGGCTCTGCACGGAGCTGAGGACAGGACAG
  ACCTTGCTTTGAGAAGGAGCTGCCGGCCGGGGCCACGCTCCACAGCCGCCGCGCGACAGTGGAGCCAAGG
  GTTAGGGCACCAGGAGGGGCCAGGTGGCGTCGGCAGCATCTGTCCCCAGAATCAGGCAGAATCCACTTCC
  CAAACAGAGCCCCACGCAGGTTCACCATGAACCTCAGGGTCAGGGAATGAGCCAGGCACGGGGGCATGGG
  CAGAGAGGGCCACGGGGCAGGGCCCACTGAGGGAACATCAGTGGCCCTCCAGTCAGGTTCTGTGGGTTTG
  GAAGCCCATCGTGAAAGGGGCTGACCTTTGCCCCTTTTTACTTGGCATTGGTTTTGAAACCAGCTGTTTC
  CCAAACTCTGCTTCCCAAGGGCAACCGTTGCTGTTCACACGCTCAGCCTGTCTGGGGGAGCGGGCCTCTA
  GCTTCAGCCAGGGCGGGTACACACCCTGGGCACAGGGTCCTCAGCCCCCGGGAAATGAGCTCCCAGGGCT
  GGCGTCCCACCTTCCAGGTGGGGGCTGGCACATCACAGACTGTCGAGAGCGCCATGTCCCAGGGCATGCA
  GAGGATGCACCTAGAGACGTTGCAGCAAGTGGACAAGTGGCCGCTGTGCGGGCCCCTCGCTTGTAGTGAG
  CTGTTGCAGCTTACGGTCCGTTCCCTGGAGGGGTGGAGGAAGGAGGTGTTGGGCAGCATCAAAGGTGCTG
  GGACATCCCAGGGTGGTGAGATCCATCCACGATCCAGCTCCGGTGGAGAAAGGGCCCATGTCAAGCCTTG
  TTCTGCACCCCAAGCATTGGTGGTAGGACTGGGTCCTGGCTGATCGTCCTTGTTCCCAGTGGGGTACATG
  TGAGCCCCTGCCAGGGCCAAGTCCTTCTCCCGAACCCAGGGTCCTGGGAACTGCAGATCCCGGGGGGATT
  CAGCCCTTCTCCCACTGTGCTGGCAGAGGCACTCCTGTGACGCTGAATACAGTGAACAGGGACATTCCCG
  CCACTCGGGGACAGATGGGCACAAGGGAGGGGAAACTCCATCAGGAAGTGCTCCCCTGGGCAGAGGCGCC
  CACTGGGTGCTGTGGGCTCAGGAGGGGGCGGGGCAGGAGCTGGTGCCAACCGGGAACCAGAGCCCCACAG
  CCATACAGCCCATTGGTGACAAGGTCCTGAGAACACAGTGGCCAGGTGTCCCCAGGCTCCTGGCCCCTCC
  GACGACCTCAACTCTGCCCAGCCCGGTCCCTGGCCATCAGCGACGCTGTCCGCCCCCCGTCAGATCCCAT
  GTGTGCCATGTTTATCATCAGTGTTTTGTATTTTTGTACTGAGTATCGGAGCACTTTACAGAAGCTGACT
  GTACATTCCTGTTCTGTTGTGAAGAGAACATTCCCAGACCCTGGCACCCTCCTGAGCCGGCGTGTGCCGG
  TCCAGCCCTCCGAGATGCCACAATTCCTTGGATGGGGGAGAAGTTCAAGGAATTTCTGCTCGGCCACGCG
  GTGGGAACCCCGCGTCCCCGCCATGTGGCAGAGGGGTCTCAGTCGTGCTAGGCATCGGGCGGCAGCGCCG
  ACAGCCCTTCCCTCGCCAGTGCCCCTCGGCCACTCCTGGGTTGGAGCCCGATTTTATTTGTAAAGTTGAC
  AGTCGAGCAAATGTTCCTATTTTCGTGGGATCTGCACACGTCTTTGTCAGTTGTGGTCATGATCTTAGTC
  ACCTGCTAATTATTTTTACAATGATTACAACATTTCCTCACTGCGGGATATTTCTGACCCGCTTTAGAAC
  TTAAGACCTGATTCTAGCAATAAACGTGTCCGAGATGAGCGGTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_015318.3
• Summary: Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GEF family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Nov 2018]
• Locus ID: 23370