Phosphoribosyl pyrophosphate amidotransferase (PPAT) (NM_002703) Human 3' UTR Clone

CAT#: SC217338

3`UTR clone of phosphoribosyl pyrophosphate amidotransferase (PPAT) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PPAT
• Synonyms: ATASE; GPAT; PRAT
• ACCN: NM_002703
• Insert Size: 2006 bp
• Sequence Data:
  Insert Sequence

  >SC217338 3'UTR clone of NM_002703
  The sequence shown below is from the reference sequence of NM_002703. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GTACAGCTTGTCTCACTGGAAAATATCCTGTAGAATTAGAATGGTAGCTGGTAGGGTTGGATGTGTGTAG
  TTTCAAGATAGAAAGTTGGTCAAGAAGTTATAGTGGTCACACCTCATCTATTTACTGTTACTCAGTTGGT
  ACAATGTAAAATGCCATGCTTATGTTTATAAGTTTTGAGATTTTTTTTTTTTCTGAAAAGGATACCAAAG
  TGCGATAACTGAACATTTCCAATTGCATATAATACAACAATATGTGGTGTTCTTTTTTTTACACAAGCAT
  TGGCTAGCCTTTTTAACCTGGTCAGAGAAGGCAGGTGGTCACTGACATTTCCCAAGTCCATGCTTTAAAG
  GGTTTGCAAGAAGTTAGGGTTAAGGAGAGGTGATGCCAACAAGACAGGTGAGTTAAATATACCATTTCAC
  ACAAAGTTTGAATAGAATACATTATACCTCATAGGTGTCTAGCCTCTACAGTTCTGGCTGTAGTTATGAC
  CTTGGCTTCCCTGTCTAACTGTAGACAAATCTTTAAAAAAAAATAATAATAATCTGGTGCCTCAGTTTCC
  CCACATGTGCAATGGGATACTTATTAAATAATTAATAAGAATGTGAATAAGTGTCATACTTTTGTGATTT
  GAGCCATCATTTCACTTCTGATTTTAAGACAACTCATGATTGTTAGCTTTCAGAAAGCTAATGATTGTTA
  ACTTTTTGAAATTAGTTTACAATTAATTAAGATTTCATTATGATGGAAGGAGACATAATTGGCAGATCTT
  TGCCATCTCTCTTTGAGATGTCCTAAAAAGGGTTGTAAAAATCTGTGAAAAAGTTTTTCCTACATTTGAC
  TAGAAAATGTGATCCATAGTATTTAGTGCCCTGATACTATAAGCTCAGCAAGTAACCTGGTACATTTGAA
  ATAAAAACCAAATTTTTAGATTCAAACAATCCCTTTATCCTTAATTTAATTAATTATCATATGCTTTTTT
  TAATGAAGTGCTTGATCACTTGCAAACATATATACATGTAGATGTACATATACATGTACACATACACATA
  AATATTATTGCAATTAAGTGATCAAGTACAGACACAATAGGGGCCAGTTTTGTTTAAGGATCAAAGAGAC
  AACCACTTTGGGGAATTAGTATCAACTTACAATCCAAGTCCAAGTATCATCTTATAATCACTTTTTTCTA
  CTATATTAAGATCTAATGAATTTGATTTCTTTTTTGAAGTTTTTTCTTGTAACATCTGAGATTAGAAGTT
  TAAGATGACTTGACCCCAAACCTTTGTTTATGTAAGAATTTTTAAACATAAAAGTGTTTGTTTCTGTTAT
  GTTACCATAATTTGATGTATATAGTGTCCAGATCCATTTAGAAATTTAATATTTATTAATAACTGAAACT
  GTTTGTCTTCCTTTGGTATATAGTCTCGCATATTATATTATAGCAGGCCAAGATAAAATTTTGACAGCTC
  TTTAAGCCCACATGCAGCAGTGGGTCAGATAACCCTGTGGCAGTGACACGGGCAAATTGGCATTTGAATA
  AAGCCCTGGGACCACCTCAACATGCGTAGCCTCTTGTCTTAAATGTACTCCCCATGGCAGCATGGAGGAG
  GCAAGACCTGTGGGTCAATTTTGAACTGGCCTTACTTTGATTTTTAAAACAAGAGACTCAGGGAAAGTAC
  TAAACCAAAATCTCTGATTTTACTTTGCGTTTTCTGTAGTTTTTGTTTTACTGAGATGCTTTTGTAAAGG
  AAAATAATACTGTGACAGTTTAGTAATTCTACAGATTCTTAATATTTCTCCATCATGGCCTTTTACTTCA
  CAATTTTCTGAAGTCTGAATTCAATTACAATTTTTTTTTACCAATTTAATCTCAAATGTTGTTTAACTGC
  TTTAAATTCATATACGTAGAGTATTATAAACTGCAGAGATGAAAAATGTGTTTTCACGGGATTTATATTG
  TGAACTAAACTAAGCCTACTTTTTGTGACTTATTTGTGATGCCTTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002703.3
• Summary: 'The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Mar 2011]'
• Locus ID: 5471