14-3-3 zeta (YWHAZ) (NM_145690) Human 3' UTR Clone

CAT#: SC217826

3`UTR clone of tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta polypeptide (YWHAZ) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: YWHAZ
• Synonyms: 14-3-3-zeta; HEL-S-3; HEL-S-93; HEL4; KCIP-1; POPCHAS; YWHAD
• ACCN: NM_145690
• Insert Size: 2159 bp
• Sequence Data:
  Insert Sequence

  >SC217826 3'UTR clone of NM_145690
  The sequence shown below is from the reference sequence of NM_145690. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATACCCAAGGAGACGAAGCTGAAGCAGGAGAAGGAGGGGAAAATTAACCGGCCTTCCAACTTTTGTCTGC
  CTCATTCTAAAATTTACACAGTAGACCATTTGTCATCCATGCTGTCCCACAAATAGTTTTTTGTTTACGA
  TTTATGACAGGTTTATGTTACTTCTATTTGAATTTCTATATTTCCCATGTGGTTTTTATGTTTAATATTA
  GGGGAGTAGAGCCAGTTAACATTTAGGGAGTTATCTGTTTTCATCTTGAGGTGGCCAATATGGGGATGTG
  GAATTTTTATACAAGTTATAAGTGTTTGGCATAGTACTTTTGGTACATTGTGGCTTCAAAAGGGCCAGTG
  TAAAACTGCTTCCATGTCTAAGCAAAGAAAACTGCCTACATACTGGTTTGTCCTGGCGGGGAATAAAAGG
  GATCATTGGTTCCAGTCACAGGTGTAGTAATTGTGGGTACTTTAAGGTTTGGAGCACTTACAAGGCTGTG
  GTAGAATCATACCCCATGGATACCACATATTAAACCATGTATATCTGTGGAATACTCAATGTGTACACCT
  TTGACTACAGCTGCAGAAGTGTTCCTTTAGACAAAGTTGTGACCCATTTTACTCTGGATAAGGGCAGAAA
  CGGTTCACATTCCATTATTTGTAAAGTTACCTGCTGTTAGCTTTCATTATTTTTGCTACACTCATTTTAT
  TTGTATTTAAATGTTTTAGGCAACCTAAGAACAAATGTAAAAGTAAAGATGCAGGAAAAATGAATTGCTT
  GGTATTCATTACTTCATGTATATCAAGCACAGCAGTAAAACAAAAACCCATGTATTTAACTTTTTTTTAG
  GATTTTTGCTTTTGTGATTTTTTTTTTTTTGATACTTGCCTAACATGCATGTGCTGTAAAAATAGTTAAC
  AGGGAAATAACTTGAGATGATGGCTAGCTTTGTTTAATGTCTTATGAAATTTTCATGAACAATCCAAGCA
  TAATTGTTAAGAACACGTGTATTAAATTCATGTAAGTGGAATAAAAGTTTTATGAATGGACTTTTCAACT
  ACTTTCTCTACAGCTTTTCATGTAAATTAGTCTTGGTTCTGAAACTTCTCTAAAGGAAATTGTACATTTT
  TTGAAATTTATTCCTTATTCCCTCTTGGCAGCTAATGGGCTCTTACCAAGTTTAAACACAAAATTTATCA
  TAACAAAAATACTACTAATATAACTACTGTTTCCATGTCCCATGATCCCCTCTCTTCCTCCCCACCCTGA
  AAAAAATGAGTTCCTATTTTTTCTGGGAGAGGGGGGGATTGATTAGAAAAAAATGTAGTGTGTTCCATTT
  AAAATTTTGGCATATGGCATTTTCTAACTTAGGAAGCCACAATGTTCTTGGCCCATCATGACATTGGGTA
  GCATTAACTGTAAGTTTTGTGCTTCCAAATCACTTTTTGGTTTTTAAGAATTTCTTGATACTCTTATAGC
  CTGCCTTCAATTTTGATCCTTTATTCTTTCTATTTGTCAGGTGCACAAGATTACCTTCCTGTTTTAGCCT
  TCTGTCTTGTCACCAACCATTCTTACTTGGTGGCCATGTACTTGGAAAAAGGCCGCATGATCTTTCTGGC
  TCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAGACTATTTCCCTCATCCTATTTA
  CTGCAGCAAATCTCTCCTTAGTTGATGAGACTGTGTTTATCTCCCTTTAAAACCCTACCTATCCTGAATG
  GTCTGTCATTGTCTGCCTTTAAAATCCTTCCTCTTTCTTCCTCCTCTATTCTCTAAATAATGATGGGGCT
  AAGTTATACCCAAAGCTCACTTTACAAAATATTTCCTCAGTACTTTGCAGAAAACACCAAACAAAAATGC
  CATTTTAAAAAAGGTGTATTTTTTCTTTTAGAATGTAAGCTCCTCAAGAGCAGGGACAATGTTTTCTGTA
  TGTTCTATTGTGCCTAGTACACTGTAAATGCTCAATAAATATTGATGATGGGAGGCAGTGAGTCTTGATG
  ATAAGGGTGAGAAACTGAAATCCCAAACACTGTTTTGTTGCTTGTTTTATTATGACCTCAGATTAAATTG
  GGAAATATTGGCCCTTTTGAATAATTGTCCCAAATATTACATTCAAATAAAAGTGCAAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_145690.2
• Summary: 'This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]'
• Locus ID: 7534