Thrombospondin 1 (THBS1) (NM_003246) Human 3' UTR Clone

CAT#: SC217830

3`UTR clone of thrombospondin 1 (THBS1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: THBS1
• Synonyms: THBS; THBS-1; TSP; TSP-1; TSP1
• ACCN: NM_003246
• Insert Size: 2126 bp
• Sequence Data:
  Insert Sequence

  >SC217830 3'UTR clone of NM_003246
  The sequence shown below is from the reference sequence of NM_003246. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGTGTTCTTCTCTGACCTGAAATACGAATGTAGAGATCCCTAATCATCAAATTGTTGATTGAAAGACTGA
  TCATAAACCAATGCTGGTATTGCACCTTCTGGAACTATGGGCTTGAGAAAACCCCCAGGATCACTTCTCC
  TTGGCTTCCTTCTTTTCTGTGCTTGCATCAGTGTGGACTCCTAGAACGTGCGACCTGCCTCAAGAAAATG
  CAGTTTTCAAAAACAGACTCAGCATTCAGCCTCCAATGAATAAGACATCTTCCAAGCATATAAACAATTG
  CTTTGGTTTCCTTTTGAAAAAGCATCTACTTGCTTCAGTTGGGAAGGTGCCCATTCCACTCTGCCTTTGT
  CACAGAGCAGGGTGCTATTGTGAGGCCATCTCTGAGCAGTGGACTCAAAAGCATTTTCAGGCATGTCAGA
  GAAGGGAGGACTCACTAGAATTAGCAAACAAAACCACCCTGACATCCTCCTTCAGGAACACGGGGAGCAG
  AGGCCAAAGCACTAAGGGGAGGGCGCATACCCGAGACGATTGTATGAAGAAAATATGGAGGAACTGTTAC
  ATGTTCGGTACTAAGTCATTTTCAGGGGATTGAAAGACTATTGCTGGATTTCATGATGCTGACTGGCGTT
  AGCTGATTAACCCATGTAAATAGGCACTTAAATAGAAGCAGGAAAGGGAGACAAAGACTGGCTTCTGGAC
  TTCCTCCCTGATCCCCACCCTTACTCATCACCTGCAGTGGCCAGAATTAGGGAATCAGAATCAAACCAGT
  GTAAGGCAGTGCTGGCTGCCATTGCCTGGTCACATTGAAATTGGTGGCTTCATTCTAGATGTAGCTTGTG
  CAGATGTAGCAGGAAAATAGGAAAACCTACCATCTCAGTGAGCACCAGCTGCCTCCCAAAGGAGGGGCAG
  CCGTGCTTATATTTTTATGGTTACAATGGCACAAAATTATTATCAACCTAACTAAAACATTCCTTTTCTC
  TTTTTTCCTGAATTATCATGGAGTTTTCTAATTCTCTCTTTTGGAATGTAGATTTTTTTTAAATGCTTTA
  CGATGTAAAATATTTATTTTTTACTTATTCTGGAAGATCTGGCTGAAGGATTATTCATGGAACAGGAAGA
  AGCGTAAAGACTATCCATGTCATCTTTGTTGAGAGTCTTCGTGACTGTAAGATTGTAAATACAGATTATT
  TATTAACTCTGTTCTGCCTGGAAATTTAGGCTTCATACGGAAAGTGTTTGAGAGCAAGTAGTTGACATTT
  ATCAGCAAATCTCTTGCAAGAACAGCACAAGGAAAATCAGTCTAATAAGCTGCTCTGCCCCTTGTGCTCA
  GAGTGGATGTTATGGGATTCTTTTTTTCTCTGTTTTATCTTTTCAAGTGGAATTAGTTGGTTATCCATTT
  GCAAATGTTTTAAATTGCAAAGAAAGCCATGAGGTCTTCAATACTGTTTTACCCCATCCCTTGTGCATAT
  TTCCAGGGAGAAGGAAAGCATATACACTTTTTTCTTTCATTTTTCCAAAAGAGAAAAAAATGACAAAAGG
  TGAAACTTACATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTTGGATCAAGCGGAAA
  GAGTTTAAGTGTCTAACAAACTTAAAGCTACTGTAGTACCTAAAAAGTCAGTGTTGTACATAGCATAAAA
  ACTCTGCAGAGAAGTATTCCCAATAAGGAAATAGCATTGAAATGTTAAATACAATTTCTGAAAGTTATGT
  TTTTTTTCTATCATCTGGTATACCATTGCTTTATTTTTATAAATTATTTTCTCATTGCCATTGGAATAGA
  TATCTCAGATTGTGTAGATATGCTATTTAAATAATTTATCAGGAAATACTGCCTGTAGAGTTAGTATTTC
  TATTTTTATATAATGTTTGCACACTGAATTGAAGAATTGTTGGTTTTTTCTTTTTTTTGTTTTGTTTTTT
  TTTTTTTTTTTTTTTGCTTTTGACCTCCCATTTTTACTATTTGCCAATACCTTTTTCTAGGAATGTGCTT
  TTTTTTGTACACATTTTTATCCATTTTACATTCTAAAGCAGTGTAAGTTGTATATTACTGTTTCTTATGT
  ACAAGGAACAACAATAAATCATATGG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003246.2
• Summary: 'The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq, Jul 2008]'
• Locus ID: 7057