MET (NM_001127500) Human 3' UTR Clone

CAT#: SC218300

3`UTR clone of met proto-oncogene (hepatocyte growth factor receptor) (MET) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MET
• Synonyms: AUTS9; c-Met; DFNB97; HGFR; RCCP2
• ACCN: NM_001127500
• Insert Size: 2306 bp
• Sequence Data:
  Insert Sequence

  >SC218300 3'UTR clone of NM_001127500
  The sequence shown below is from the reference sequence of NM_001127500. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGATGAGGTGGACACACGACCAGCCTCCTTCTGGGAGACATCATAGTGCTAGTACTATGTCAAAGCAACA
  GTCCACACTTTGTCCAATGGTTTTTTCACTGCCTGACCTTTAAAAGGCCATCGATATTCTTTGCTCTTGC
  CAAAATTGCACTATTATAGGACTTGTATTGTTATTTAAATTACTGGATTCTAAGGAATTTCTTATCTGAC
  AGAGCATCAGAACCAGAGGCTTGGTCCCACAGGCCACGGACCAATGGCCTGCAGCCGTGACAACACTCCT
  GTCATATTGGAGTCCAAAACTTGAATTCTGGGTTGAATTTTTTAAAAATCAGGTACCACTTGATTTCATA
  TGGGAAATTGAAGCAGGAAATATTGAGGGCTTCTTGATCACAGAAAACTCAGAAGAGATAGTAATGCTCA
  GGACAGGAGCGGCAGCCCCAGAACAGGCCACTCATTTAGAATTCTAGTGTTTCAAAACACTTTTGTGTGT
  TGTATGGTCAATAACATTTTTCATTACTGATGGTGTCATTCACCCATTAGGTAAACATTCCCTTTTAAAT
  GTTTGTTTGTTTTTTGAGACAGGATCTCACTCTGTTGCCAGGGCTGTAGTGCAGTGGTGTGATCATAGCT
  CACTGCAACCTCCACCTCCCAGGCTCAAGCCTCCCGAATAGCTGGGACTACAGGCGCACACCACCATCCC
  CGGCTAATTTTTGTATTTTTTGTAGAGACGGGGTTTTGCCATGTTGCCAAGGCTGGTTTCAAACTCCTGG
  ACTCAAGAAATCCACCCACCTCAGCCTCCCAAAGTGCTAGGATTACAGGCATGAGCCACTGCGCCCAGCC
  CTTATAAATTTTTGTATAGACATTCCTTTGGTTGGAAGAATATTTATAGGCAATACAGTCAAAGTTTCAA
  AATAGCATCACACAAAACATGTTTATAAATGAACAGGATGTAATGTACATAGATGACATTAAGAAAATTT
  GTATGAAATAATTTAGTCATCATGAAATATTTAGTTGTCATATAAAAACCCACTGTTTGAGAATGATGCT
  ACTCTGATCTAATGAATGTGAACATGTAGATGTTTTGTGTGTATTTTTTTAAATGAAAACTCAAAATAAG
  ACAAGTAATTTGTTGATAAATATTTTTAAAGATAACTCAGCATGTTTGTAAAGCAGGATACATTTTACTA
  AAAGGTTCATTGGTTCCAATCACAGCTCATAGGTAGAGCAAAGAAAGGGTGGATGGATTGAAAAGATTAG
  CCTCTGTCTCGGTGGCAGGTTCCCACCTCGCAAGCAATTGGAAACAAAACTTTTGGGGAGTTTTATTTTG
  CATTAGGGTGTGTTTTATGTTAAGCAAAACATACTTTAGAAACAAATGAAAAAGGCAATTGAAAATCCCA
  GCTATTTCACCTAGATGGAATAGCCACCCTGAGCAGAACTTTGTGATGCTTCATTCTGTGGAATTTTGTG
  CTTGCTACTGTATAGTGCATGTGGTGTAGGTTACTCTAACTGGTTTTGTCGACGTAAACATTTAAAGTGT
  TATATTTTTTATAAAAATGTTTATTTTTAATGATATGAGAAAAATTTTGTTAGGCCACAAAAACACTGCA
  CTGTGAACATTTTAGAAAAGGTATGTCAGACTGGGATTAATGACAGCATGATTTTCAATGACTGTAAATT
  GCGATAAGGAAATGTACTGATTGCCAATACACCCCACCCTCATTACATCATCAGGACTTGAAGCCAAGGG
  TTAACCCAGCAAGCTACAAAGAGGGTGTGTCACACTGAAACTCAATAGTTGAGTTTGGCTGTTGTTGCAG
  GAAAATGATTATAACTAAAAGCTCTCTGATAGTGCAGAGACTTACCAGAAGACACAAGGAATTGTACTGA
  AGAGCTATTACAATCCAAATATTGCCGTTTCATAAATGTAATAAGTAATACTAATTCACAGAGTATTGTA
  AATGGTGGATGACAAAAGAAAATCTGCTCTGTGGAAAGAAAGAACTGTCTCTACCAGGGTCAAGAGCATG
  AACGCATCAATAGAAAGAACTCGGGGAAACATCCCATCAACAGGACTACACACTTGTATATACATTCTTG
  AGAACACTGCAATGTGAAAATCACGTTTGCTATTTATAAACTTGTCCTTAGATTAATGTGTCTGGACAGA
  TTGTGGGAGTAAGTGATTCTTCTAAGAATTAGATACTTGTCACTGCCTATACCTGCAGCTGAACTGAATG
  GTACTTCGTATGTTAATAGTTGTTCTGATAAATCATGCAATTAAAGTAAAGTGATGCAACATCTTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001127500.1
• Summary: 'This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016]'
• Locus ID: 4233