IRS2 (NM_003749) Human 3' UTR Clone

CAT#: SC218912

3`UTR clone of insulin receptor substrate 2 (IRS2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: IRS2
• Synonyms: IRS-2
• ACCN: NM_003749
• Insert Size: 2456
• Sequence Data:
  Insert Sequence

  >SC218912 3'UTR clone of NM_003749
  The sequence shown below is from the reference sequence of NM_003749. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCACCATCGTGAAAGAGTGAAGATCTGTCTGGCTTTATCACCAGGATGTCACATGTCAGAGAGTATCATT
  AAAAGAAGACGCTCAGCACTGTTTCAGCCCGAAGCTGCTTGCAGTTTTCTTTTGGATCTGAGCAATGACT
  GTGTTTGGAAACATCTGTGGACTCTGTTAGATGAGGCACCAACAAGGCAAGGTCACCTGCCTCTTTCCCT
  TGTTCCCGGATGGGGCATTCATCATTGTGCTGTTTGCGTTTTGTTTTGTTTTGTTTTAACAAAATTAGCT
  GAAGAAGTTATTCTCAAGAAAATTGGATGTTTTCATTGGCCTTCTTAAATTGTGGCCAGTGTCTTTTAAT
  TTCTTCTTCTTTTCCTTTTGGCAAAGCAGATATAACCCTCAGCATGCTAGGAGAGTGCACCCGTACCTAT
  GGAAGTGGTAAAATCTGGTATTTACTGGCTTACACTCAAAACGACCACAGTCCTACCTCAGTTCAAGGTA
  AAGCCGGATTTCCGTGGCGGGGGTCCCACAGGACCTCCTGTAGTAGCCCCTGCGCTGTGTGTCTGGAGCG
  CGGTCCTCGGCCTTATTGAAATGGTCCAAGTAGACAGCTGCTTGTTGGATTCCAGTGCAGGTACCTGCGA
  TGTTTACGTCCACACCGAGCCCAGTGTGGGACTGACATTTCTCAATGGAAGTGAAATTTGGGATTGGACT
  TTGAAGACGGATTACTAAATAATAATTATTATATGTAACTGAAGCAACCTACTTTTGAAAATCAACTGTA
  TTGGGTAGTGGGAGGTGGGAGGGAAGGGCTTTGGGAAGGGGATGAATATCTCTTTTTACCTTTAACAGAC
  TTGTTTAATCTTCTCGATGTAGATGTTTATGTAGGTACTTCACATTGCAAACGCCTTTTATTCTATTTAC
  AAGCTCAGATGTCTCTGCTCTCCTGAATCTTGGGCATGCCTTTCTGTAACCAAAAATCCCTGTAGGCGTG
  CTAGCAATTCCAGGGTGGTCCGGGTTTGGCAGATTTGATTTTTAAAAAACGTATTATCTTTAATAAAATG
  TTATTATGTCAACCAGTGAGGCTGCCCTGAACAAAAAAAACAAAAAGAAAAAAAAAAAAGGAAAGAAAGA
  AACTGATAAAAAGAGGCATTCCAGCCCCTATGTTATTGATGGAAAAAGAAAAAGAAGAAAAGCAATCTCG
  CAGTACATGTTACTTGTCGAAAAAATTCCGGACAAGACTACCCTTGTTTTATGTTTTCAGTATTCTGAAA
  ATACCAGTGTGTGGCAGTTCTCGCAGATGTTACCTAAAACTGCTGAACTTGACCGGCAGAATGTTCTGCC
  GTTTTCTGCTCCCTCGACACTTGATTGGAGGGCTGTCGACCTCTCCTCCCGTGGGGGCTTCCCCAGTGCC
  TATCTTCTCTGATAGTCATGGAGAGGTTACACTAATTCATTGGAGATGTAAGTTGTTGGTTTTGTTTTGT
  TTTGTTTTTAGAAAAATATATATAAATATATAATAGATATCTATCGCTATAGAATAATGCATTAATAAAA
  TGAGGCTTTTTTAGAGGAAGACCAAAAAATTCAATGTCTTAAAAATATATTTAATGGCAATGCAAAAGTC
  TTCCTGCTTCCGTGCTGAACTTTAGAACAGAGGATTGTATTGCAAGACAAAGTTGAATGTAAAGTGATCT
  CCCTGAACATTTTTAAGGTTTTACTTTTCTGAAATTATACATCACAGCAGTGCATAGGCCATATAATGTT
  AGCTGGAAGGTCAATTTCAGTGTATGATATACTTTATTAAGATGTATAAAAATCCTGAAGTTTTTATTTA
  GTTTTGGGAATAGGCATCAATGGGTGGTATTTGCTTTGTAACTCCCCCCAGGTACGATAGGGACTGAATA
  TGGACCCTGCTGAAAGCAGTGTATTGACGCATATTTAACTCGCCCTCTATCCGTAGAGTAGTCATGACAC
  TATACAGATGGTTCGTGTTCATACTGCAGCTTAAAACAAGCAAAATACACAGATGATAATATGCTAAATT
  TTCCTCTATCCTGTACATTTCACAAAAAGGCATATGCAATATTTACATTTTTAATTTAGTTTACAGAATG
  GAACCAAAATGTATAAATGTTATGTTTGCTAAAACTTCACAATGTATATTGGGTCTTTGTACATTTTGCC
  TGACTTACCTTAAATTTAAAATATTTTTTGCTATATAAACTTTAACAGTTATTAAACAGTGTTTTCTTTT
  TGGGTACGTATTGTTTCTGGATATCAAGATGTTAAATATATTTCTTGCTATTGTGATATGACAAGAGACT
  TAACTTATCTTGCTCTGTCTTCCACTGTACACGCTGTATATAGGGGTCAATGTGATGCTGCTGGAGACGA
  GAATAAACTGGACTAGAATAGTGCATTGTATTTAGTCTGTATTGATCATGGATGCCCTCCTTAATAGCCA
  TATGCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_003749.2
• Summary: This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq, Jul 2008]
• Locus ID: 8660