IRAK4 (NM_001114182) Human 3' UTR Clone

CAT#: SC219805

3`UTR clone of interleukin-1 receptor-associated kinase 4 (IRAK4) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: IRAK4
• Synonyms: IPD1; IRAK-4; NY-REN-64; REN64
• ACCN: NM_001114182
• Insert Size: 2839
• Sequence Data:
  Insert Sequence

  >SC219805 3'UTR clone of NM_001114182
  The sequence shown below is from the reference sequence of NM_001114182. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTGCAAGAGATGACAGCTTCTTAAAACTTTATTGGAAAAGACTCTTGACTTTTTATATACACCTATCTCA
  ACCATTTTTTTAACTGATTTTTTTCCTAAATATTCTTCTTTACCTTTAACAAGGCATAGGCTGTTGCAGG
  ACAGTGGTTATTAAAGCATGGGTTGAACTTCCAAAATATAAAAATAGAGCCACCATATCAACACTTAGCC
  CTACCCATTAGTATCACCCCCAGTTCTTACAGTAATCCCTGAGAAATCTCCTTCAAGCATCACCAAACAC
  AGTTTGAAAATTACAGGGTTAGCAAAAAGAGCCTGGGCTGTATGTAGGGTGGAAACACTCTGATCTGAAG
  CCCAGCTGACTCCACTACTAATTTGCTGTAAAGCTTTGGACATACACTTAGCTGCTGTGAGCCACTAATA
  ACATTGGGCTAATATCTGCTGTGCTTCTCTGACAGGTAGTCATGAAAATCAAATGATGCAAAATATATAC
  AAGCACTTTGTAAATTGTAAAATGATACAAAATTTAAAGTTTATAGAGCCAGTTACAAAATCCTATTAGT
  CATATATTTATAGATTGTGTTCACAGCAATCATTTAACCACAAATAAAATATCCCTTGATGATACTGCCA
  TAATGATATGTCCATTATTAGATTATGTTACATGACAAAGTTGAAGGAATTTGGCAGATGCAGTTAAGGT
  TCCTAAACAACTCACTTTGAGACTGTTGAAAGGGCCTGACCTAATCAAGTGAACCCTTGCAAGAAGAATT
  CTCCTTGTAAGCCTTGAAGAAGTATGTGAGAGGGCCACATTGGCTAAAACCTAAAGGTGGCCTCTAGGAG
  ATGAGACCTACCTTCCAGTTGTCAGCAAGCAGGAAAAAAAAATTGGGACCTCAGTTGCAACCACAAGGAA
  CTGAATTCTGCCAAAAATCTGAGTCAGCTTAGAAGAGTACTCCAAGCTTCAGATGATAACCACAGCCTGG
  GCTGACACCTGGATTTCAGCTTTGCATGATCCTCAGTATGAGAATCTATCTGTTCTGTGCTGGACTTCTA
  ATATATAGAACTGTGAGATAATGGGTCACATTGGCTGGATGTGGTGGCTCATACCTGTAAATCCCAGCAC
  TTTGGGAGGCCGAGGCAGGCAGATCACCTGAGGTCAAGAGTTCAAGACCGGCCTGGCCAACATGGTGAAA
  CCCCGTCTCTACTAAAAATACAAAAATTAGACGAGCGTGGTGGTGGACACCTGTAGTCCCAGCTACTTGG
  GAGGCTGAGGCAGGAGACTAGCTGGAACCAGGGAGGTAGAGGTTGCAGTGAGCTGAGATCGTGCCACTGC
  ACTCCAGCCTGGGTGACAGAGTGAGACTCCATCATAAATAAATAAATAAATAAATGGGTCACATTAAGCC
  TTTAAGTTTGTGGTAATTTATTATTCAGTAATAGAAAACAAATACAGATACTCTCCCATGATGTTTTTCC
  CATGATGATTTCCCATGATATTTACAGGTTTTGCCCACATTTGAGGGGTATGTGGAAATTATACAGAGCA
  TGTACAGCGGGAGGCTTATAGTGTACGTACTGAAATGTGGGGTTGGAGCCCCAACACAGAGACCCCAGCA
  GGACACTGCCTAGTAGAGCTATGGGAAGGGTGCTGCCACCCTCCAGACTTGAGAATTGTAGAGCCACCAG
  CAGCTTGCACTCTGAGCTTGGAAAAGCCACAGGCACTCAACTTCAACCATGAGGGAAGCCACGCACCCTG
  CAAAGCCACAGGAGTGGAGCTGCCCACGGCCTCGAGGGCCCACCCCTTGCACCAGTGTGCCAGGATGTGG
  GACATGGAATCAAGGAATATGTTAGGGCTTTTTTTTTTTTTTTTTTGAGACGGAGTCTTGCTCTGTTGCC
  CAGGCTGGAGTGCAGTGGCACGATCTCGGCTCACTGCAAGCTCTGCCTCCCAGGTTCACGCCATTCCCCT
  GCCTCAGCCTCCCCAGTAGCTGGGACTACAGGTGCCCGCCACCATGCCCAGCTAATTTTTTTTGTATTTT
  TAGTAGAGATGGGGTTTCACTGTGTTAGCCAGGATGGTCTTGATCTCCTGACCTCGTGATCACCCGCCTC
  GGCCTCCCAAAGTGCCAGTATTTAAAGTTTAATGTCTTCCCAGCTGGGTTTCAGACTTGCCAGGATCCTG
  TTGCCCCTTTCTTTAGCCAATTTCTCCCTTTTGGGACAAGAATGTTTTACTTATTGCCTGTACCACCAAC
  TGTATCTTGGAAATAAATAACTTATATTTTATTTCAGAGGCTCATAGGCGGCAGGAACTTACCTTGAGTC
  TCAAATGAGACTTAGGACTTTTGAGTGATGCTAGAATGAGTTAAGACTTTGGGAAGGGATGATTATATTT
  TGCAATGTGAGAAAGACATTAGATTTGGGGGGCTGGGGGTAGAATGACATTGTTTAGATGTTTGTCTCCT
  TCAAATTTCATGTTTAAATGTAATCCCCAGTGTTGGGGGTGGAGGTGGGGCCTGATGGGAAGTGTTTGGG
  TCATGGTGGATGATCCCTCATGAATGGCTTAGAGCCACTGGTGATGAGTGAGTTCTCACTCAGTTCGTGT
  GAGATCTGGTGGCTTAAAAGAGTCTGCTCCCCCTTTGCCTTCCATTGTGATTGTAACCTTCCTGGGGCCT
  TCATCAAAGGCCAGGCAAATATTGGTGCCGTGCTAGTATGGCCTGCAGAACCGTAAGCCAAAACAAACCT
  TTTTTCTTTATAAATTACCCAGCCTCAGTTATTCCTTTATAGTGATGCAAAACGGGCTAACTATATAAAT
  TTGTAATTTTTAAGTTATAGATTGTTTTTATTAAACACT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_001114182.2
• Summary: This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
• Locus ID: 51135