E2F3 (NM_001949) Human 3' UTR Clone

CAT#: SC220669

3`UTR clone of E2F transcription factor 3 (E2F3) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: E2F3
• Synonyms: E2F-3
• ACCN: NM_001949
• Insert Size: 3325 bp
• Sequence Data:
  Insert Sequence

  >SC220669 3'UTR clone of NM_001949
  The sequence shown below is from the reference sequence of NM_001949. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AAAAGCTCCCACTGGTGGAAGACTTCATGTGTAGTTGATTATGCTTCGTGTGAACTCTCCTTAAAAACCG
  ATATTTTTTTATCATGGAACCAGAACATCTGTCATGCAGTGTTGTCCCTTCCTACCTTCTTCCTCCAAGA
  GAGTATCATGAAGTAAACTACAAACTTCAGAAGAAAGCTGACATTTTAATGAATTTTTTAAAAAATTAAT
  AAACAAATTGTCTAAACGCACAGTTGCAGGCTCCCTTGGGAAAGCCCTGCTTTGCTCCAGGCTCCAAGAT
  CTCCTGGCTAAGTCAGCAAGTGAGAAAATGTGCAATCAGGTGTCTCTCACCCCGAATTGTCCTTCCTCCT
  TCCTCCCCGGATTGGCTTGCTGTGCCTGACGGATGGGCTGTAGAATGGGGTCTGGCCACCTGGCCTGCTG
  GGAAACAGCAATCTTCCTTAATAGCATTTCAAGCCGTGCCTTCTCCGCAGAATGCATGTCTTTGAGGTCT
  GCTAATATGGAATGGAACTGCAGCAAATGCAAACTTGAAGTCATGCAAAAGTATGAAATGGATTTCTTCA
  GCTCTTCTTAGGAATATTTAAATTACTGTCATAATTCAGTTTAAGCTATGAACTGTGTGTCCCAGTAGGA
  GGTCAAGAAAACCTCCACAGCCTTCTGGATGAAGAACCTGTTTTCAAATATACTTGTTGCAGATACAGAA
  GACTAGTAGAGTTCTGCCACTCTAAGCTGTTGTGGATTTTCCTGTCTCCATGAACCACTCCCATTCCCCC
  GTCCCCAATGTGTTTGTGAGTTTCCAGTTGATTTGTAGCAAATGCCTACTTAGTTCTTTGTGGATTGTTC
  TAGACTTTTAATTTTTTTAGCTGCCATTTAAGCATTCCTGTGGCACCCATCACCATTTCAATTTAATTGT
  TTACTTTGAAGCGGTTTTTGCAAATTCATATTACTTAAGCAGAGGGAGAGAACCTCTACTGATCAGAGCA
  TCTAAACCTGTGTGATCTAAGGTTTATCAGCCTCTGCAAGGAGCTTTGTCCCATCGTGCTTCCATTCCCA
  GGAGGGGGAGCTTGGAGCGAGTCAGTCCTGGGGCTTGCTGACATGGGTGGCCCATTGGAAAGGAGAACCA
  GGTCAGATGATGTAACAGCCCCAAGGAGCAGCAGGCATGGGTCCCTCCATCCTTGGGCTTCCCGGGCCCC
  TGTGACAGGGGAAAGGGCTCTCTTACACCGCACTCAGGGAGACCACTTCTCAGGATGGGGTCAGATGGAG
  AGACCTCTAGGGAGAAAGACATCCCCATTGTGTGAGTGGCATTTCCTTAAGCTGGCAGGAACAGGGAGCA
  GCCCTGTGTCGGGGGCTGGAATAGTTCTGGCCAGACCCCGTTCCCCTTCCTCTATGAAGGAATAAGTTGG
  ACCAAGGGAAGTCGGGGACGTAAAAAATGAAGCAAAACAATGCCAGGGTGTCTCCCGCTTTACTCTTCAG
  GAATGGTGTCCCAAGTTGGAGGCTTTGTGTCAGCTGCAAATCCTACCAGTTATGTCCAAGAATGGCTTTC
  CCTCGGGCAGGTGGCAGCGGCCATCTCCCACTGGGAATATGGCGTAGTATCTCCGGTCCATTCCTTGGAT
  GCTAAGGACTGCGGGAATGAGGGAGTCAGATAAAGAACAAACCTCGAAACGAACAGTTAAATTGAAATGC
  TATGTGCCTGACCCAATGGTAGGCACATAGTAGGCACTCAACTCATATGTTTAATTGAATTGAAAATATC
  CCTTAGGAAAAAAAAAAAACACACAAAAAACCACAAGAGCCCCAGCCAGTTTACTCCAGGTAGATTTCCA
  CAATATGCAAAGTGGTGGTGGGGTCAAGACAGATGACACCAGCACTTTAAACTCTTTGTGTGGGTATGCG
  TGGGTGTATGTTTGGGAAGAAAAACAAAGGTGCAGACTATCTTCCTTTTTTTCTTCTTCAGCCTCCATCC
  CTGGCCTCCTCCCCTCACACACACTGGACTTGGTACAAAATGTCGGTGTGGTCCTAGATGAAGCATTGGG
  GTGGGGGAGGGAGAGGGAGCTTTGTGTTAAGTGCCTACTGGAAATGCACTGTGGGGTTTTTTCCTGTATG
  GGAAACCATTTATGCCAAGCTTTTCCCCATTTCCCATATTTATCTCATCTGGTTAGCTGCCTCTGCTTCC
  AGCTTTGTGTAATTCTCTTTGCCAGCTGCACAAAGCTGATTTTTTCCAAAGTCTAAAGACTGAGCTCACC
  TGGCTAGATTGTTGTGTGTTTTGTTGAATTTTTTCATAATGTAATGCCGTATTTATTGTTTTTAAAATGA
  AAGGAATACTAATAAGTCTTAAAAGTTCCTTCATGCATAAGATTTTTTTCCAGTTACTGGGTTTAACTGG
  TGTACATTAATTAGATGTCCATACTGTATTTTGTTTGCATTAAGTAATTTTCTTTTTGACTTAGTATCCG
  GCACACAAAGTGGGTTAGTACTACAGTATTTGCGTTACTTTAAGTACTAAGTATGCAGGTTTCCTGGTAC
  CATTGAGTTGCTGCTATTAAAGCTCACACACGAAATGGCTAAAAGTTACAAGTGTGCAAATTATGACTGC
  GTGAGCCTTAGAAAATAAAATGTATAAAGGGCAACACATGAGCTGTCAAACAGTGTTAGGAGTGTGTTTA
  TATGTACAGAGTTGTGCATAGCAATCGTTTTATTTAAGTTGATATGTAGTCTACTCACATTTTCATTATT
  TAGCAATTTTGTACAAAAATAGCAATTAATTTGTAAACACTGCCAGAATACTTTCTAGCTGCTTTGTAAT
  TTTTTAAGAGTGTTATTTTGTTTTTGTTTTTCTGTTCTTTGTTGTGGCTCTTGTTTTCATTTTTGTTGTA
  CGTGTAGATCTGTAAATAAAATTGCAGTATTTAAAGCTTAAGCTTTCAGGAAAAAGAAAATAAGAATTCA
  GTGTGTGCATGACAACTCGTGTGTATGAGAAGGAGGGATATGAAGGAAGATGGCTTGCAGAGTAAGTCGG
  GTGGCAATTGTCAGGGTGTGGGAATTTCTTTTCCTACGGGGTACGTGATTTTGTAAAAAGGAAGTATTTC
  TCCCAAAATTGGGAGTAGGCAAACTACTAATCAGTTTAGCTTTGTGTTGTATGCTAGTTTAAAAAAGAAA
  ATATGTAATATAATGTAAAAAAAAACAAAAAAAAGCTTTTATGATGGATTTTGTAAATAGATTTGTTACA
  GGGTGACCTGTTCTCTAGCTGTGATCTTACCACTTCAAATGGGTGTAATTTGAATAAATTTTGTATGGTA
  AAGGATCAATAAAATGATTTTTTTTAAGAGTTAAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001949.3
• Summary: 'This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]'
• Locus ID: 1871