GREM2 (NM_022469) Human 3' UTR Clone

CAT#: SC220786

3`UTR clone of gremlin 2 cysteine knot superfamily homolog (Xenopus laevis) (GREM2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GREM2
• Synonyms: CKTSF1B2; DAND3; PRDC; STHAG9
• ACCN: NM_022469
• Insert Size: 3426
• Sequence Data:
  Insert Sequence

  >SC220786 3'UTR clone of NM_022469
  The sequence shown below is from the reference sequence of NM_022469. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATGTCCGTGAACCTGAGCGACTCGGACAAGCAGTGAGCGCCGGGCCGGACGCAGCTCAGCCCCGCGCGCG
  CCCGGCCGCTGGGTGGCGCCGCCGCCGCCTCTGTCCCTGCCCTCCGAGCCCACTGTCACGCTGCCTGGTG
  TTCCCCCTCAGCAGCAAGCACTTCTCTTAGGGCTGACGGTGTCCTTGTCACAGACGTGGATCGCAAGTGG
  AGCTTTTGCTGATGTGTTCCTGACCGACCCCGGGTCCCACCTGTGCCCCCTAAACCAGGCGGTGGATCAG
  GCCCGAAGGAAAATGCTGGAAAACCACCACCGCCACCCAGCAGTCAGAGAGGACCTTTCGAGGTGATGCA
  CTGTAAATCCGGCATTGATGTTTCCACCATGTGGAAAAGCAAGGATTTCCCTGGCCCCGCCCTGCACTCC
  AGCTCGCCCCTTTCCCGCGCGAAGTGAAGGATGCGTCACTTTTCTGAGCGGCCCCTCAGACCTCTCCTGC
  TGGTCCCTGGACCTCTGGCTGGGAGCGCTCATTCTTGTGACTGGCAGGCTGCCCTCGGTCGCTGCTGTGA
  TGAGGATATGTGCAACCTCCATAAATATGTCGGTGGACGGGGCGTCTCCTAATAAACCTGATACCAAGAA
  GACAACTTGAGCCACTTGGATCCCGAAGTGGGCTTCCCGGGAAACCTCGAGCAGAGCAGGCTACACAATC
  CCCCATCCTCAAGTCCCCACCGCCTCTTCTTCCTCACTGTCCCCGCCCCCTATTTGTGGACTAAAGATGA
  ACTCTGGTGTGCATGCTATTATTGCTGCAATTAGAATATTATCACACACAAAGGTCTCTATATTAACGCT
  GGTTTTATAAGTGACCGTATATTGTAAAGAGCTGTTAAAAAGTATTATAGACCTATTCATGTATTATTTC
  ACATGTTTTGACTCTGGCTTGCAGCACCATTCGGAGTAAGGATGACAGCAGGCCCAGAAGGTGTCTTACT
  CAGAAAAAAAAATGCCTGGTCAGCAATTCACTGTCATGCACCTATTTTTAGATTGGGCAGGGAGATGGGA
  GGAGTCGTGATTTTTACTTTGAATACTATTTCACTGACGTTACTAAGCATTGCAGCACAATGTAGAAATT
  GGCTTGGGATGGATAGGTATAGGGAAGGGCATTCGTTGGGAAATTGATGACCGGAAACTAGAAAGCCCCT
  ATAATGTGGAATATGCATATGTGGGGTGAAACCGTATACTACTTTCACTGCCATGACACTAGGCAAAAAA
  TATTTCCTGTTAAAAGAAAGAGAAAAAGAGAGGGATATTGAAAAATATAAGGAGTTGAGTCGCTTTGTGG
  TATAGAAGGGAGTAAGGAATAAAAGATATAGAATAGTAATCTAGTTGAACTGTTCTGCACAAAACATGCT
  TCCTTTTCAAAGAATAGACTTCAAAAGGGACGAACAAACATTAGGTCACCTTTTGTGTTTATAATGAATT
  GTTGACGTTTCCATGCTGTGGTCTCTTGATATACGGAAGTAGACGTAACTTATTTTCCAGCGAAATTATA
  TACTTCTTCTGGGCTCCTTTGCTCAGTTGCCCCAGGAAGGCTTCCCTGTGAGTAATGCTTATGGAATCTG
  ACAAATGTGAATGAGCTGCCATTGGTGGGAATCAGTAAACTTTGAAAACCTTTCACTGATGATTCCCTGG
  TAAGTGCTCCTGTGAGGGGCCACTTCCCTAGTATGTGAGAGAGACCCTCTGCAAGCCGCTGTGGGGACTT
  AGCTTCCTGATACTAGGTAGTGATGGTGGATCTAGAGACATTCACTTGAAGAGCAAGGATTGGGGTGGGG
  TGGGTTGGTGAATCTATCTCAGGCCACTGCACTGCAAGGTGCATTTCTGTCATTTCTGCAACACCATGAG
  ACCTGAGCAAGAATGTGCTGTGGCAGAGGGGGTCTTGGTTTCATAAATGGGTATATACAGCCTGAACTCA
  GGAGGCAGAAATCAAAAGAAATCCTTGAGGGCTGGAACCTGAGGATAGAGCGAAAATGGAGAGAAGGGTG
  TTCAAGGTCAGGAGTGATTTTTGCATTTGGTGGAAAACCTCAAGTCAGACTCAGTGGCAGAACATGCAGC
  AGAAAAGCTGTGAAGGAAGGAAATTTACGTGATTGTAATGGAGGCTAGGGGTGGATTGGGAGGTGAGTGG
  AGGAGAGTCTCTGACCAGCTGCAGAGCCAACCCCTATCATTTTATTAACATGGAACCCACACAGATAGGG
  GAGGGATTAAGCTAAGAACTTACAGAATGCAAACACGAGCACACTCTCTTCGAACCCAATTGTGGGTGTA
  GCAATGAAAGCAATATGATATGCTGCAGTGTGAAGCTCCTTCTGGGGTTATCGTATGTACAAAGTTTACC
  TTATAATGGCTCAAATTGTATTTAATTTTTTTGTTTTGTTTATAAATGAAGAAAAGCTATAAGTATAATG
  TAATTATTTTATAGGTATACTATTAAATTATAGAACAAATAAAGATAATCTAGGATTATATGTGATCCCG
  GTGTGACGTGCCATAAAAGTGGAATTTTTGACTTGTTGCAGTGAGCTGTGAAGCAGCACTATGCGTTCAG
  AGAGTTTTTGTTCAACCATGGTGAGTTCAACCTCTAGCTTATGTCACATGATCTCCTTTGGGGTCTGGAA
  TCTTCTAAGAGAAATAGAATCAGGCATCCAAGGAGCAGAAGTGATGGAGGTGGCCCTCAATCCTGGTCTT
  TGGAAGCTTCCAAGCCTCTTCCACATCCCCTGCCAGCTGAAGACAGAGTATTCTCACTGTGCTGGCTGAA
  GGAGAAACACTAAACAAGGAAACCTTGAGAAACTGCAGCAATGGAAGTCCACTGTTAGTCTCTCCGTTAG
  CACCAGGGACACACTTGTTCTGAGTTTTGTTCATTTTCACATTTACTCCTCATGAGTTCCAACATTTCTT
  AATTTAAATATTTCTTAGTTTATAGCATCTCCATTTTTCTATTAATTGACTTAATCTGCCATTTAAGAAA
  ACTCCGTAATCAATAAAAAATAAAATCCACAATCAAACTGCACATGGTTGATCCACTGTGTAGGCTTCAA
  AGGAATTAAACTCAACTTCCTAGGAAATAATTCATCTTAGTTTATTAAATGGGACAGAATATTTCACTGC
  TAACGTATTCTAACTCACTTGTTTTTGAAATTGGCCAGATGATTTTTAAGTTATAAAGTCAATGAAAATG
  ACTTTTGCTATTGTGTGAACAACTTAGAATGTATATATTCCCAGAAGGCTTATTTAATGATTGACCTATC
  ACCACTGATTATTAATGTCTGCATGAATGATAACAATAAGACCAAGTCATCAATAATGCAATGAAAATTT
  TTTAAGTGAAAGCAAGTTTATTAAGAAAGTAAAGGAATAAAGAATGCCTACTTCATAGGCAGAGCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_022469.3
• Summary: This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. [provided by RefSeq, Jul 2008]
• Locus ID: 64388