DYRK1A (NM_101395) Human 3' UTR Clone

CAT#: SC220938

3`UTR clone of dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A (DYRK1A) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DYRK1A
• Synonyms: DYRK; DYRK1; HP86; MNB; MNBH; MRD7
• ACCN: NM_101395
• Insert Size: 3535 bp
• Sequence Data:
  Insert Sequence

  >SC220938 3'UTR clone of NM_101395
  The sequence shown below is from the reference sequence of NM_101395. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCATTCTCAGGTGGAGCAGCACTGGATGCCAGGTGCCTTTAGAATGACCGTATCATTTACCCTAGAGGTT
  CATGACGTTCCTGTCTAAGGAAATGTGCGTCAGCAATTTCCTGCTCCTCTTGGTTGGTCAGGCACTGAAG
  CTCCTACACAGGTCACTGTTGAAACTCATCCTGTTCAAGAAACAACCTTTCATGTAGCCCCTCAACAGAA
  TGCATTGCATCATCACCATGGTAACAGTTCCCATCACCATCACCACCACCACCACCATCACCACCACCAT
  GGACAACAAGCCTTGGGTAACCGGACCAGGCCAAGGGTCTACAATTCTCCAACGAATAGCTCCTCTACCC
  AAGATTCTATGGAGGTTGGCCACAGTCACCACTCCATGACATCCCTGTCTTCCTCAACGACTTCTTCCTC
  GACATCTTCCTCCTCTACTGGTAACCAAGGCAATCAGGCCTACCAGAATCGCCCAGTGGCTGCTAATACC
  TTGGACTTTGGACAGAATGGAGCTATGGACGTTAATTTGACCGTCTACTCCAATCCCCGCCAAGAGACTG
  GCATAGCTGGACATCCAACATACCAATTTTCTGCTAATACAGGTCCTGCACATTACATGACTGAAGGACA
  TCTGACAATGAGGCAAGGGGCTGATAGAGAAGAGTCCCCCATGACAGGAGTTTGTGTGCAACAGAGTCCT
  GTAGCTAGCTCGTGACTACATTGAAACTTGAGTTTGTTTCTTGTGTGTTTTTATAGAAGTGGTGTTTTTT
  TTCCAAAAACAAAGTGCAAAGCTGCTTGAATCAGGAGGAGATTAACACACTGAACCGCTACAAGAGGGCA
  AAGCTGATTTTTTTTTTAACTTGAAAAGATTGCAAAGGGACATTGAAGTGTTTAAAAGAGCCATGTCCAA
  ACCCATCTTCATGGATAGCTCAGAGGTATCCTCTTTTTGCTCCCCCATTTTAACTTGCCACATCCCAGTC
  ACAGTGGGGTTTTTTTGTCTTTCTATTCAGCAAAAGTTAATATTCAGATGTTGGTCTTGGTCATTTGCCA
  ACTAATTTTAAAGTAAAAGGCACTGCACATAATTTGCATAAAGGGCCCCATGAGGGTGTTTTTTTTTTTT
  CTTTTTGTCCCCCCCATCCCCCTTTTTTTTTGTTTTGTTCTGTTTTGTTTTGGGTGGGAGGGTGGGAAAT
  TTGGGTTTTTAAGTCCTCTAAACACACTTGGGCACGGAAATGCAGTACTGTAAGGAAGAGGGACCTCCAG
  CTTCCACAAACACCATCTTCAGCTGTATGAAAGGGACGGTTGTGGTGAAGTTTGTCAGGCACAGTAAGCA
  TGCTGAGTGGCGGGGATCAGAACTCTCCTATCTGAACCTACTGAGGAGCAAAGCAGCAATTACATGGGAT
  CCTGTGGCTCTCCCGTTGCAGAGGCCACAGGAAGATAGGATGGAACGTGACTGGTCTCCTAACCAAGGTG
  CACTGAGAAGCAATCAACGGGTCGGTCGTGGCCAGTCCTGGGGAGGTCTGAGTGGTGGTCTTTGGGATAA
  CCTTTGGCCTTATGGATTTGGACTCGAAATTAGAAGAGCCTACCATTTCAGATGCAATCACTTTTGGACA
  TGCTTTTGCAGACAGTCCTTAATGCTGAAAACACAGAGAATGGGTAATTCAAGAGGCCTTTCTTTTAAAA
  TAGACTTTTGTGACCCACTAATTGTAAGGTATTGCAAGGTCACTTTGCGTGTGTCATAAAGTTGACTTCC
  TTATTGGTTGAAGGTCACAGAAGTAGTGGTTTGCTTTGATGGAAATAGCTACAGCTGTGTCCCTTCCTGC
  TTTTTACTTTTTCTTTTGCTTTTTCTCGGCACGTGGTATCTCCACCATTTCTTCTGCACAAAGATGTCTT
  CTGTTCATCCTGAACATTTTTAAAAAATGCAGAATTTTATGTGACTGCTTTTTTGCCTCACAATTATGCT
  GTGAATTTTACAAAAATTTATTTTCTTTTTTGATAATTTATTGTACCAAAGCTGTTTTTATAGCACATAG
  ATGTCTGTAACCAATAATGTAGCAGTTCTGCACTTTGACACAAGGTGTAACTAGACCATTTTTAAATGTC
  AGTTGAAAATTATGGCTGTACTATTGCTTAAACAAAACTGGAACTGTTGTTGAATCCATAGCCAATACAT
  TTACAGCAATCTGTGTACTGAACATAGTAGATTGACATCTAATTCAAGATTACAACATCTGTTACATTCT
  AAGTGTGTTCAGGCTTCTGAAGGTAAAGGGACACTGGATCCAGAAGCTATGGAACCAGCAGTTGATTCTT
  GTATTCCTGATTAACCTACTTGTAAACTTGAAAGCAAGACCTTGATTGCACCAACAGGTCCAGAGTATGA
  GTGCAAGCAAAGCAGAACTCTCATGCGTGACCTGAGCAGACAGGCTGGTATTTAACAGGTGCCTCGTGTT
  GAGATTACGCTGCCTTAATGTAACACAGTCTGGCAGTTGCTAAATTTGTGTTCCCATTTTAAATTGACCA
  ATTTTGGGGTGTGACACTTTTGAGCGGTTGAATTGGGAGAATGAAGATAAGTAATTTACCTGTCCAGGAT
  CAAAAGAAGCCTAGAAAAGAAGCAGTAATCTACCTCTGCCGATAACCTGTTTAAGATGACTCAGCAGAAC
  ACCGCGTTTCATTCTATTGGTCAATTCCATGTGGCTGACTAGGTCAATTTTTTTTCTGAACAAAAGCAGG
  TTTTTATATGTAAACAGTGAGAAAAGAAAGGCTAAACACTATGTAAATGTGAATGGAAACTTGGAAATAC
  TCGTTTTTATAAACTACAAAAACTTTTTGTTGTTTATCAGGAAATCCATATTTATTTTGTAATTAACTGT
  CAAGCCTGTGGATGATTTTTTTGAACTTGGTAGTTCATAAAGGTTTACAGTGAATAAAAGGATATCATCT
  TGAGTATAGCAATATCAAAAGGAATTCAGTAGTTACTGCTGTTTAGGAATATAAGGTTAAGATATCATAT
  GGGTCAGGTCATTTTTTTTTTCTGTGCTGGTTGCCACATCTTAGCAAGCACCAAAAAACTAAAGCAGTTT
  TTAAACCGATATTTACGTAAAGAAAATCATAAAATCCAATGCTTCTGCATACTGTGTTATGTTACAGTCC
  AGTTTTGTGTGCTTTACTACACAGTTTGGTTACAGGACTTCTGTGCATTGTAAACATAAACAGCATGGAA
  AAGGTTAAATACCTGTGTTCAGATTGTAAGATCTAGTCCGGACTTGCTGTGTATATTGTAACGTTAAATG
  AAAAAAGAACCCCCCTTTGTATTATAGTCATGCGGTCTTATGTATGATAAACAGTTGAATAATTTGTCCT
  CAGACTCTTTACTATGCTTTTTTAAAAATTAATTTAAGAAAAATGTAAACATAGTAAAAATCTTCCTATG
  CAATTAAACTGGTCCAGGTCTGGTAGGTATAGTATCAAAGTTGAGTTAAATGTGTAAAAAGGAAACTATT
  TGAGATACATTGACATAGGCATCAGCAATCTCTGA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_101395.2
• Summary: 'This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms. [provided by RefSeq, Jul 2008]'
• Locus ID: 1859