ATM (NM_000051) Human 3' UTR Clone

CAT#: SC221017

3`UTR clone of ataxia telangiectasia mutated (ATM) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ATM
• Synonyms: AT1; ATA; ATC; ATD; ATDC; ATE; TEL1; TELO1
• ACCN: NM_000051
• Insert Size: 3602 bp
• Sequence Data:
  Insert Sequence

  >SC221017 3'UTR clone of NM_000051
  The sequence shown below is from the reference sequence of NM_000051. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGCCGACTTTTCCCAGGATGGAAAGCTTGGGTGTGATCTTCAGTATATGAATTACCCTTTCATTCAGCCT
  TTAGAAATTATATTTTAGCCTTTATTTTTAACCTGCCAACATACTTTAAGTAGGGATTAATATTTAAGTG
  AACTATTGTGGGTTTTTTTGAATGTTGGTTTTAATACTTGATTTAATCACCACTCAAAAATGTTTTGATG
  GTCTTAAGGAACATCTCTGCTTTCACTCTTTAGAAATAATGGTCATTCGGGCTGGGCGCAGCGGCTCACG
  CCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGAGCGGATCACAAGGTCAGGAGTTCGAGACCAGCCTGG
  CCAAGAGACCAGCCTGGCCAGTATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCGAGCATG
  GTGGCGGGCACCTGTAATCCCAGCTACTCGAGAGGCTGAGGCAGGAGAATCTCTTGAACCTGGGAGGTGA
  AGGTTGCTGTGGGCCAAAATCATGCCATTGCACTCCAGCCTGGGTGACAAGAGCGAAACTCCATCTCAAA
  AAAAAAAAAAAAAAAACAGAAACGTATTTGGATTTTTCCTAGTAAGATCACTCAGTGTTACTAAATAATG
  AAGTTGTTATGGAGAACAAATTTCAAAGACACAGTTAGTGTAGTTACTATTTTTTTAAGTGTGTATTAAA
  ACTTCTCATTCTATTCTCTTTATCTTTTAAGCCCTTCTGTACTGTCCATGTATGTTATCTTTCTGTGATA
  ACTTCATAGATTGCCTTCTAGTTCATGAATTCTCTTGTCAGATGTATATAATCTCTTTTACCCTATCCAT
  TGGGCTTCTTCTTTCAGAAATTGTTTTTCATTTCTAATTATGCATCATTTTTCAGATCTCTGTTTCTTGA
  TGTCATTTTTAATGTTTTTTTAATGTTTTTTATGTCACTAATTATTTTAAATGTCTGTACTTGATAGACA
  CTGTAATAGTTCTATTAAATTTAGTTCCTGCTGTTTATATCTGTTGATTTTTGTATTTGATAGGCTGTTC
  ATCCAGTTTTGTCTTTTTGAAAAGTGAGTTTATTTTCAGCAAGGCTTTATCTATGGGAATCTTGAGTGTC
  TGTTTATGTCATATTCCCAGGGCTGTTGCTGCACACAAGCCCATTCTTATTTTAATTTCTTGGCTTTAGG
  GTTTCCATACCTGAAGTGTAGCATAAATACTGATAGGAGATTTCCCAGGCCAAGGCAAACACACTTCCTC
  CTCATCTCCTTGTGCTAGTGGGCAGAATATTTGATTGATGCCTTTTTCACTGAGAGTATAAGCTTCCATG
  TGTCCCACCTTTATGGCAGGGGTGGAAGGAGGTACATTTAATTCCCACTGCCTGCCTTTGGCAAGCCCTG
  GGTTCTTTGCTCCCCATATAGATGTCTAAGCTAAAAGCCGTGGGTTAATGAGACTGGCAAATTGTTCCAG
  GACAGCTACAGCATCAGCTCACATATTCACCTCTCTGGTTTTTCATTCCCCTCATTTTTTTCTGAGACAG
  AGTCTTGCTCTGTCACCCAGGCTGGAGTGCAGTGGCATGATCTCAGCTCACTGAAACCTCTGCCTCCTGG
  GTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGTGTGCCAACACGCCCGGCT
  AATTTTTTGTATTTTTATTAGAGACGGAGTTTCACCGTGTTAGCCAGGATGGTCTCGATCGCTTGACCTC
  GTGATCCACCCTCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCGCCCGGCCTCATTC
  CCCTCATTTTTGACCGTAAGGATTTCCCCTTTCTTGTAAGTTCTGCTATGTATTTAAAAGAATGTTTTCT
  ACATTTTATCCAGCATTTCTCTGTGTTCTGTTGGAAGGGAAGGGCTTAGGTATCTAGTTTGATACATAGG
  TAGAAGTGGAACATTTCTCTGTCCCCCAGCTGTCATCATATAAGATAAACATCAGATAAAAAGCCACCTG
  AAAGTAAAACTACTGACTCGTGTATTAGTGAGTATAATCTCTTCTCCATCCTTAGGAAAATGTTCATCCC
  AGCTGCGGAGATTAACAAATGGGTGATTGAGCTTTCTCCTCGTATTTGGACCTTGAAGGTTATATAAATT
  TTTTTCTTATGAAGAGTTGGCATTTCTTTTTATTGCCAATGGCAGGCACTCATTCATATTTGATCTCCTC
  ACCTTCCCCTCCCCTAAAACCAATCTCCAGAACTTTTTGGACTATAAATTTCTTGGTTTGACTTCTGGAG
  AACTGTTCAGAATATTACTTTGCATTTCAAATTACAAACTTACCTTGGTGTATCTTTTTCTTACAAGCTG
  CCTAAATGAATATTTGGTATATATTGGTAGTTTTATTACTATAGTAAATCAAGGAAATGCAGTAAACTTA
  AAATGTCTTTAAGAAAGCCCTGAAATCTTCATGGGTGAAATTAGAAATTATCAACTAGATAATAGTATAG
  ATAAATGAATTTGTAGCTAATTCTTGCTAGTTGTTGCATCCAGAGAGCTTTGAATAACATCATTAATCTA
  CTCTTTAGCCTTGCATGGTATGCTATGAGGCTCCTGTTCTGTTCAAGTATTCTAATCAATGGCTTTGAAA
  AGTTTATCAAATTTACATACAGATCACAAGCCTAGGAGAAATAACTAATTCACAGATGACAGAATTAAGA
  TTATAAAAGATTTTTTTTTTGTAATTTTAGTAGAGACAGGGTTGCCATTGTATTCCAGCCTTGGCGACAG
  AGCAAGACTCTGCCTCAAAAAAAAAAAAAAAAAGGTTTTGGCAAGCTGGAACTCTTTCTGCAAATGACTA
  AGATAGAAAACTGCCAAGGACAAATGAGGAGTAGTTAGATTTTGAAAATATTAATCATAGAATAGTTGTT
  GTATGCTAAGTCACTGACCCATATTATGTACAGCATTTCTGATCTTTACTTTGCAAGATTAGTGATACTA
  TCCCAATACACTGCTGGAGAAATCAGAATTTGGAGAAATAAGTTGTCCAAGGCAAGAAGATAGTAAATTA
  TAAGTACAAGTGTAATATGGACAGTATCTAACTTGAAAAGATTTCAGGCGAAAAGAATCTGGGGTTTGCC
  AGTCAGTTGCTCAAAAGGTCAATGAAAACCAAATAGTGAAGCTATCAGAGAAGCTAATAAATTATAGACT
  GCTTGAACAGTTGTGTCCAGATTAAGGGAGATAATAGCTTTCCCACCCTACTTTGTGCAGGTCATACCTC
  CCCAAAGTGTTTACCTAATCAGTAGGTTCACAAACTCTTGGTCATTATAGTATATGCCTAAAATGTATGC
  ACTTAGGAATGCTAAAAATTTAAATATGGTCTAAAGCAAATAAAAGCAAAGAGGAAAAACTTTGGACAGC
  GTAAAGACTAGAATAGTCTTTTAAAAAGAAAGCCAGTATATTGGTTTGAAATATAGAGATGTGTCCCAAT
  TTCAAGTATTTTAATTGCACCTTAATGAAATTATCTATTTTCTATAGATTTTAGTACTATTGAATGTATT
  ACTTTACTGTTACCTGAATTTATTATAAAGTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000051.3
• Summary: 'The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]'
• Locus ID: 472