RUNX1 (NM_001001890) Human 3' UTR Clone

CAT#: SC221763

3`UTR clone of runt-related transcription factor 1 (RUNX1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RUNX1
• Synonyms: AML1; AML1-EVI-1; AMLCR1; CBF2alpha; CBFA2; EVI-1; PEBP2aB; PEBP2alpha
• ACCN: NM_001001890
• Insert Size: 4323 bp
• Sequence Data:
  Insert Sequence

  >SC221763 3'UTR clone of NM_001001890
  The sequence shown below is from the reference sequence of NM_001001890. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GTGTGGAGGCCCTACTGAGGCGCCAGGCCTGGCCCGGCTGGGCCCCGCGGGCCGCCGCCTTCGCCTCCGG
  GCGCGCGGGCCTCCTGTTCGCGACAAGCCCGCCGGGATCCCGGGCCCTGGGCCCGGCCACCGTCCTGGGG
  CCGAGGGCGCCCGACGGCCAGGATCTCGCTGTAGGTCAGGCCCGCGCAGCCTCCTGCGCCCAGAAGCCCA
  CGCCGCCGCCGTCTGCTGGCGCCCCGGCCCTCGCGGAGGTGTCCGAGGCGACGCACCTCGAGGGTGTCCG
  CCGGCCCCAGCACCCAGGGGACGCGCTGGAAAGCAAACAGGAAGATTCCCGGAGGGAAACTGTGAATGCT
  TCTGATTTAGCAATGCTGTGAATAAAAAGAAAGATTTTATACCCTTGACTTAACTTTTTAACCAAGTTGT
  TTATTCCAAAGAGTGTGGAATTTTGGTTGGGGTGGGGGGAGAGGAGGGATGCAACTCGCCCTGTTTGGCA
  TCTAATTCTTATTTTTAATTTTTCCGCACCTTATCAATTGCAAAATGCGTATTTGCATTTGGGTGGTTTT
  TATTTTTATATACGTTTATATAAATATATATAAATTGAGCTTGCTTCTTTCTTGCTTTGACCATGGAAAG
  AAATATGATTCCCTTTTCTTTAAGTTTTATTTAACTTTTCTTTTGGACTTTTGGGTAGTTGTTTTTTTTT
  GTTTTGTTTTGTTTTTTTGAGAAACAGCTACAGCTTTGGGTCATTTTTAACTACTGTATTCCCACAAGGA
  ATCCCCAGATATTTATGTATCTTGATGTTCAGACATTTATGTGTTGATAATTTTTTAATTATTTAAATGT
  ACTTATATTAAGAAAAATATCAAGTACTACATTTTCTTTTGTTCTTGATAGTAGCCAAAGTTAAATGTAT
  CACATTGAAGAAGGCTAGAAAAAAAGAATGAGTAATGTGATCGCTTGGTTATCCAGAAGTATTGTTTACA
  TTAAACTCCCTTTCATGTTAATCAAACAAGTGAGTAGCTCACGCAGCAACGTTTTTAATAGGATTTTTAG
  ACACTGAGGGTCACTCCAAGGATCAGAAGTATGGAATTTTCTGCCAGGCTCAACAAGGGTCTCATATCTA
  ACTTCCTCCTTAAAACAGAGAAGGTCAATCTAGTTCCAGAGGGTTGAGGCAGGTGCCAATAATTACATCT
  TTGGAGAGGATTTGATTTCTGCCCAGGGATTTGCTCACCCCAAGGTCATCTGATAATTTCACAGATGCTG
  TGTAACAGAACACAGCCAAAGTAAACTGTGTAGGGGAGCCACATTTACATAGGAACCAAATCAATGAATT
  TAGGGGTTACGATTATAGCAATTTAAGGGCCCACCAGAAGCAGGCCTCGAGGAGTCAATTTGCCTCTGTG
  TGCCTCAGTGGAGACAAGTGGGAAAACATGGTCCCACCTGTGCGAGACCCCCTGTCCTGTGCTGCTCACT
  CAACAACATCTTTGTGTTGCTTTCACCAGGCTGAGACCCTACCCTATGGGGTATATGGGCTTTTACCTGT
  GCACCAGTGTGACAGGAAAGATTCATGTCACTACTGTCCGTGGCTACAATTCAAAGGTATCCAATGTCGC
  TGTAAATTTTATGGCACTATTTTTATTGGAGGATTTGGTCAGAATGCAGTTGTTGTACAACTCATAAATA
  CTAACTGCTGATTTTGACACATGTGTGCTCCAAATGATCTGGTGGTTATTTAACGTACCTCTTAAAATTC
  GTTGAAACGATTTCAGGTCAACTCTGAAGAGTATTTGAAAGCAGGACTTCAGAACAGTGTTTGATTTTTA
  TTTTATAAATTTAAGCATTCAAATTAGGCAAATCTTTGGCTGCAGGCAGCAAAAACAGCTGGACTTATTT
  AAAACAACTTGTTTTTGAGTTTTCTTATATATATATTGATTATTTGTTTTACACACATGCAGTAGCACTT
  TGGTAAGAGTTAAAGAGTAAAGCAGCTTATGTTGTCAGGTCGTTCTTATCTAGAGAAGAGCTATAGCAGA
  TCTCGGACAAACTCAGAATATATTCACTTTCATTTTTGACAGGATTCCCTCCACAACTCAGTTTCATATA
  TTATTCCGTATTACATTTTTGCAGCTAAATTACCATAAAATGTCAGCAAATGTAAAAATTTAATTTCTGA
  AAAGCACCATTAGCCCATTTCCCCCAAATTAAACGTAAATGTTTTTTTTCAGCACATGTTACCATGTCTG
  ACCTGCAAAAATGCTGGAGAAAAATGAAGGAAAAAATTATGTTTTTCAGTTTAATTCTGTTAACTGAAGA
  TATTCCAACTCAAAACCAGCCTCATGCTCTGATTAGATAATCTTTTACATTGAACCTTTACTCTCAAAGC
  CATGTGTGGAGGGGGCTTGTCACTATTGTAGGCTCACTGGATTGGTCATTTAGAGTTTCACAGACTCTTA
  CCAGCATATATAGTATTTAATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACGC
  AACACATTTTTGTATGTGTGTGTGTGTGCGTGTGTGTGTGTGTGTGTGAAAAATTGCATTCATTGACTTC
  AGGTAGATTAAGGTATCTTTTTATTCATTGCCCTCAGGAAAGTTAAGGTATCAATGAGACCCTTAAGCCA
  ATCATGTAATAACTGCATGTGTCTGGTCCAGGAGAAGTATTGAATAAGCCATTTCTACTGCTTACTCATG
  TCCCTATTTATGATTTCAACATGGATACATATTTCAGTTCTTTCTTTTTCTCACTATCTGAAAATACATT
  TCCCTCCCTCTCTTCCCCCCAATATCTCCCTTTTTTTCTCTCTTCCTCTATCTTCCAAACCCCACTTTCT
  CCCTCCTCCTTTTCCTGTGTTCTCTTAAGCAGATAGCACATACCCCCACCCAGTACCAAATTTCAGAACA
  CAAGAAGGTCCAGTTCTTCCCCCTTCACATAAAGGAACATGGTTTGTCAGCCTTTCTCCTGTTTATGGGT
  TTCTTCCAGCAGAACAGAGACATTGCCAACCATATTGGATCTGCTTGCTGTCCAAACCAGCAAACTTTCC
  TGGGCAAATCACAATCAGTGAGTAAATAGACAGCCTTTCTGCTGCCTTGGGTTTCTGTGCAGATAAACAG
  AAATGCTCTGATTAGAAAGGAAATGAATGGTTCCACTCAAATGTCCTGCAATTTAGGATTGCAGATTTCT
  GCCTTGAAATACCTGTTTCTTTGGGACATTCCGTCCTGATGATTTTTATTTTTGTTGGTTTTTATTTTTG
  GGGGGAATGACATGTTTGGGTCTTTTATACATGAAAATTTGTTTGACAATAATCTCACAAAACATATTTT
  ACATCTGAACAAAATGCCTTTTTGTTTACCGTAGCGTATACATTTGTTTTGGGATTTTTGTGTGTTTGTT
  GGGAATTTTGTTTTTAGCCAGGTCAGTATTGATGAGGCTGATCATTTGGCTCTTTTTTTCCTTCCAGAAG
  AGTTGCATCAACAAAGTTAATTGTATTTATGTATGTAAATAGATTTTAAGCTTCATTATAAAATATTGTT
  AATGCCTATAACTTTTTTTCAATTTTTTTGTGTGTGTTTCTAAGGACTTTTTCTTAGGTTTGCTAAATAC
  TGTAGGGAAAAAAATGCTTCTTTCTACTTTGTTTATTTTAGACTTTAAAATGAGCTACTTCTTATTCACT
  TTTGTAAACAGCTAATAGCATGGTTCCAATTTTTTTTAAGTTCACTTTTTTTGTTCTAGGGGAAATGAAT
  GTGCAAAAAAAGAAAAAGAACTGTTGGTTATTTGTGTTATTCTGGATGTATAAAAATCAATGGAAAAAAA
  TAAACTTTCAAATTGAAATGACGGTATAACACATCTACTGAAAAAGCAACGGGAAATGTGGTCCTATTTA
  AGCCAGCCCCCACCTAGGGTCTATTTGTGTGGCAGTTATTGGGTTTGGTCACAAAACATCCTGAAAATTC
  GTGCGTGGGCTTCTTTCTCCCTGGTACAAACGTATGGAATGCTTCTTAAAGGGGAACTGTCAAGCTGGTG
  TCTTCAGCCAGATGACATGAGAGAATATCCCAGAACCCTCTCTCCAAGGTGTTTCTAGATAGCACAGGAG
  AGCAGGCACTGCACTGTCCACAGTCCACGGTACACAGTCGGGTGGGCCGCCTCCCCTCTCCTGGGAGCAT
  TCGTCGTGCCCAGCCTGAGCAGGGCAGCTGGACTGCTGCTGTTCAGGAGCCACCAGAGCCTTCCTCTCTT
  TGTACCACAGTTTCTTCTGTAAATCCAGTGTTACAATCAGTGTGAATGGCAAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001001890.2
• Summary: 'Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
• Locus ID: 861