Hamartin (TSC1) (NM_000368) Human 3' UTR Clone

CAT#: SC222100

3`UTR clone of tuberous sclerosis 1 (TSC1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TSC1
• Synonyms: LAM; TSC
• ACCN: NM_000368
• Insert Size: 4894 bp
• Sequence Data:
  Insert Sequence

  >SC222100 3'UTR clone of NM_000368
  The sequence shown below is from the reference sequence of NM_000368. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGACTACAATGAGACTCATCATGAACACAGCTAAGGAATGATGGTCAATCAGTGTTAACTTGCATATTGT
  TGGCACAGAACAGGAGGTGTGAATGCACGTTTCAAAGCTTTCCTGTTTCCAGGGTCTGAGTGCAAGTTCA
  TGTGTGGAAATGGGACGGAGGTCCTTTGGACAGCTGACTGAATGCAGAACGGTTTTTGGATCTGGCATTG
  AAATGCCTCTTGACCTTCCCCTCCACCCGCCCTAACCCCCTCTCATTTACCTCGCAGTGTGTTCTAATCC
  AAGGGCCAGTTGGTGTTCCTCAGTAGCTTTACTTTCTTCCTTTCCCCCCCAAATGGTTGCGTCCTTTGAA
  CCTGTGCAATATGAGGCCAAATTTAATCTTTGAGTCTAACACACCACTTTCTGCTTTCCCGAAGTTCAGA
  TAACTGGGTTGGCTCTCAATTAGACCAGGTAGTTTGTTGCATTGCAGGTAAGTCTGGTTTTGTCCCTTCC
  AGGAGGACATAGCCTGCAAAGCTGGTTGTCTTTACATGAAAGCGTTTACATGAGACTTTCCGACTGCTTT
  TTTGATTCTGAAGTTCAGCATCTAAAGCAGCAGGTCTAGAAGAACAACGGTTTATTCATACTTGCATTCT
  TTTGGCAGTTCTGATAAGCTTCCTAGAAAGTTCTGTGTAAACAGAAGCCTGTTTCAGAAATCTGGAGCTG
  GCACTGTGGAGACCACACACCCTTTGGGAAAGCTCTTGTCTCTTCTTCCCCCACTACCTCTTATTTATTT
  GGTGTTTGCTTGAATGCTGGTACTATTGTGACCACAGGCTGGTGTGTAGGTGGTAAAACCTGTTCTCCAT
  AGGAGGGAAGGAGCAGTCACTGGGAGAGGTTACCCGAGAAGCACTTGAGCATGAGGAACTGCACCTTTAG
  GCCATCTCAGCTTGCTGGGCCTTTTGTTAAACCCTTCTGTCTACTGGCCTCCCTTTGTGTGCATACGCCT
  CTTGTTCATGTCAGCTTATATGTGACACTGCAGCAGAAAGGCTCTGAAGGTCCAAAGAGTTTCTGCAAAG
  TGTATGTGACCATCATTTCCCAGGCCATTAGGGTTGCCTCACTGTAGCAGGTTCTAGGCTACCAGAAGAG
  GGGCAGCTTTTTCATACCAATTCCAACTTTCAGGGGCTGACTCTCCAGGGAGCTGATGTCATCACACTCT
  CCATGTTAGTAATGGCAGAGCAGTCTAAACAGAGTCCGGGAGAATGCTGGCAAAGGCTGGCTGTGTATAC
  CCACTAGGCTGCCCCACGTGCTCCCGAGAGATGACACTAGTCAGAAAATTGGCAGTGGCAGAGAATCCAA
  ACTCAACAAGTGCTCCTGAAAGAAACGCTAGAAGCCTAAGAACTGTGGTCTGGTGTTCCAGCTGAGGCAG
  GGGGATTTGGTAGGAAGGAGCCAGTGAACTTGGCTTTCCTGTTTCTATCTTTCATTAAAAAGAATAGAAG
  GATTCAGTCATAAAGAGGTAAAAAACTGTCACGGTACGAAATCTTAGTGCCCACGGAGGCCTCGAGCAGA
  GAGAATGAAAGTCTTTTTTTTTTTTTTTTTTTTTTAGCATGGCAATAAATATTCTAGCATCCCTAACTAA
  AGGGGACTAGACAGTTAGAGACTCTGTCACCCTAGCTATACCAGCAGAAAACCTGTTCAGGCAGGCTTTC
  TGGGTGTGACTGATTCCCAGCCTGTGGCAGGGCGTGGTCCCAACTACTCAGCCTAGCACAGGCTGGCAGT
  TGGTACTGAATTGTCAGATGTGGAGTATTAGTGACACCACACATTTAATTCAGCTTTGTCCAAAGGAAAG
  CTTAAAACCCAATACAGTCTAGTTTCCTGGTTCCGTTTTAGAAAAGGAAAACGTGAACAAACTTAGAAAG
  GGAAGGAAATCCCATCAGTGAATCCTGAAACTGGTTTTAAGTGCTTTCCTTCTCCTCATGCCCAAGAGAT
  CTGTGCCATAGAACAAGATACCAGGCACTTAAAGCCTTTTCCTGAATTGGAAAGGAAAAGAGGCCCAAGT
  GCAAAAGAAAAAACATTTTAGAAACGGACAGCTTATAAAAATAAAGGGAAGAAAGGAGGCAGCATGGAGA
  GAGGCCTGTGCTAGAAGCTCCATGGACGTGTCTGCACAGGGTCCTCAGCTCATCCATGCGGCCTGGGTGT
  CCTTTTACTCAGCTTTATAACAAATGTGGCTCCAAGCTCAGGTGCCTTTGAGTTCTAGGAGGCTGTGGGT
  TTTATTCAACTACGGTTGGGAGAATGAGACCTGGAGTCATGTTGAAGGTGCCCAACCTAAAAATGTAGGC
  TTTCATGTTGCAAAGAACTCCAGAGTCAGTAGTTAGGTTTGGTTTGGTTTTGGACATGATAAACCTGCCA
  AGAGTCAACAGGTCACTTGATCATGCTGCAGTGGGTAGTTCTAAGGATGGAAAGGTGACAGTATTACTCT
  CGAGAGGCAATTCAGTCCTGGGCAAAGGTATTAGTACAATAAGCGTTAAGGGCAGAGTCTACCTTGAAAC
  CAATTAAGCAGCTTGGTATTCATAAATATTGGGATTGGATGGCCTCCATCCAGAAATCACTATGGGTGAG
  CATACCTGTCTCAGCTGTTTGGCCAATGTGCATAACCTACTCGGATCCCCACCTGACACTAACCAGAGTC
  AGCACAGGCCCCGAGGAGCCCGAAGTCTGCTGCTGTGCAGCATGGAATTCCTTTAAAAAGGTGCACTACA
  GTTTTAGCGGGGAGGGGGATAGGAAGACGCAGAGCAAATGAGCTCCGGAGTCCCTGCAGGTGAATAAACA
  CACAGATCTGCATCTGATAGAACTTTGATGGATTTTCAAAAAGCCGTTGACAAGGCTCTGCTATACAGTC
  TATAAAAATTGTTATTATGGGATTGGAAGAAACACGTGGTCATGAATAGAAAAAAAACAAACCCAAAGGT
  AGGAAGGTCAAGGTCATTTCTTAGATGGAGAAGTTGTGAAAGATGTCCTTGGAGATGAGTTTTAGGACCA
  GCATTACTAAGGCAGGTGGGCAGACAGTGACCTCTCTAGGTGTGTCCACAGAGTTTTTCAGGAGAGAAAA
  CTGCCTGACCTTTGGGACTAAGCTGCGGAATCTTCTTACTAAGCTTGAAGAGTGGAGAGGCGAGAGGTGA
  GCTACTTTGTGAGCCAAAGCTTATGTGACATGGTTGGGGAAACAGTCCAAACTGTTCTGAGAAGGTGAAC
  TGTTACGACCCAGGACAATTAGAAAAATTCACCCACCATGCCGCACATTACTGGGTAAAAGCAGGGCAGC
  AGGGAACAAAACTCCAGACTCTTGGGCCGTCCCCATTTGCAACAGCACACATAGTTTCTGGTATATTTGT
  TGGGAAAGATAAAACTCTAGCAGTTGTTGAGGGGAGGATGTATAAAATGGTCATGGGGATGAAAGGATCT
  CTGAGACCACAGAGGCTCAGACTCACTGTTAAGAATAGAAAACTGGGTATGCGTTTCATGTAGCCAGCAG
  AACTGAAGTGTGCTGTGACAAGCCAATGTGAATTTCTACCAAATAGTAGAGCATACCACTTGAAGAAGGA
  AAGAACCGAAGAGCAAACAAAAGTTCTGCGTAATGAGACTCACCTTTTCTCGCTGAAAGCACTAAGAGGT
  GGGAGGAGGCCTGCACAGGCTGGAGGAGGGTTTGGGCAGAGCGAAGACCCGGCCAGGACCTTGGTGAGAT
  GGGGTGCCGCCCACCTCCTGCGGATACTCTTGGAGAGTTGTTCCCCCAGGGGGCTCTGCCCCACCTGGAG
  AAGGAAGCTGCCTGGTGTGGAGTGACTCAAATCAGTATACCTATCTGCTGCACCTTCACTCTCCAGGGTA
  CATGCTTTAAAACCGACCCGCAACAAGTATTGGAAAAATGTATCCAGTCTGAAGATGTTTGTGTATCTGT
  TTACATCCAGAGTTCTGTGACACATGCCCCCCAGATTGCTGCAAAGATCCCAAGGCATTGATTGCACTTG
  ATTAAGCTTTTGTCTGTAGGTGAAAGAACAAGTTTAGGTCGAGGACTGGCCCCTAGGCTGCTGCTGTGAC
  CCTTGTCCCATGTGGCTTGTTTGCCTGTCCGGGACTCTTCGATGTGCCCAGGGGAGCGTGTTCCTGTCTC
  TTCCATGCCGTCCTGCAGTCCTTATCTGCTCGCCTGAGGGAAGAGTAGCTGTAGCTACAAGGGAAGCCTG
  CCTGGAAGAGCCGAGCACCTGTGCCCATGGCTTCTGGTCATGAAACGAGTTAATGATGGCAGAGGAGCTT
  CCTCCCCACTTCGCAGCGCCACATTATCCATCCTCTGAGATAAGTAGGCTGGTTTAACCATTGGAATGGA
  CCTTTCAGTGGAAACCCTGAGAGTCTGAGAACCCCCAGACCAACCCTTCCCTCCCTTTCCCCACCTCTTA
  CAGTGTTTGGACAGGAGGGTATGGTGCTGCTCTGTGTAGCAAGTACTTTGGCTTATGAAAGAGGCAGCCA
  CGCATTTTGCACTAGGAAGAATCAGTAATCACTTTTCAGAAGACTTCTATGGACCACAAATATATTACGG
  AGGAACAGATTTTGCTAAGACATAATCTAGTTTTATAACTCAATCATGAATGAACCATGTGTGGCAAACT
  TGCAGTTTAAAGGGGTCCCATCAGTGAAAGAAACTGATTTTTTTTAACGGACTGCTTTTAGTTAAATTGA
  AGAAAGTCAGCTCTTGTCAAAAGGTCTAAACTTTCCCGCCTCAATCCTAAAAGCATGTCAACAATCCACA
  TCAGATGCCATAAATATGAACTGCAGGATAAAATGGTACAATCTTAGTGAATGGGAATTGGAATCAAAAG
  AGTTTGCTGTCCTTCTTAGAATGTTCTAAAATGTCAAGGCAGTTGCTTGTGTTTAACTGTGAAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000368.4
• Summary: 'This gene is a tumor suppressor gene that encodes the growth inhibitory protein hamartin. The encoded protein interacts with and stabilizes the GTPase activating protein tuberin. This hamartin-tuberin complex negatively regulates mammalian target of rapamycin complex 1 (mTORC1) signalling which is a major regulator of anabolic cell growth. This protein also functions as a co-chaperone for Hsp90 that inhibits its ATPase activity. This protein functions as a facilitator of Hsp90-mediated folding of kinase and non-kinase clients, including Tsc2 and thereby preventing their ubiquitination and proteasomal degradation. Mutations in this gene have been associated with tuberous sclerosis. [provided by RefSeq, Apr 2018]'
• Locus ID: 7248