Bckdhb (NM_199195) Mouse Untagged Clone

CAT#: MC204787

Bckdhb (untagged) - Mouse branched chain ketoacid dehydrogenase E1, beta polypeptide (Bckdhb), nuclear gene encoding mitochondrial protein, (10ug)


  "NM_199195" in other vectors (3)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Bckdhb
Synonyms BCKDE1B; BCKDH E1-beta
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC064099
CAGAATTGGATGGCTGAATTTGAACAGATCCTTCGGGAATTGAGACTTCAGGTCAACTCCACGCGCTTGG ACCTGTCGCTGACCAAAGGATTACCCAATTGGATCTCCTCAGCATTTTCTTTCTTTAAAAAATAGGGCAA ACTCAGAAGATGAACCTCTTCCAGTCAATAACAAGTGCCCTGGATAACTCATTAGCCAAAGACCCCACTG CAGTAATATTTGGTGAAGATGTTGCCTTTGGTGGAGTCTTCCGATGCACTGTTGGTTTACGAGACAAATA CGGAAAAGATAGAGTGTTTAACACCCCGTTGTGTGAACAAGGAATAGTTGGATTTGGCATTGGAATCGCG GTCACCGGTGCTACAGCTATTGCGGAAATCCAGTTTGCCGACTATATTTTCCCTGCCTTTGATCAGATTG TCAACGAAGCTGCCAAGTATCGCTACCGCTCAGGTGATCTTTTCAACTGTGGGAGCCTCACCATCCGGGC CCCGTGGGGTTGTGTGGGCCATGGGGCTCTCTACCATTCTCAGAGTCCTGAAGCCTTTTTTGCCCATTGC CCAGGGATCAAGGTGGTAATACCCCGAAGCCCTTTCCAGGCCAAGGGACTTCTGTTGTCATGCATAGAAG ATAAAAATCCATGTATATTTTTTGAACCTAAAATACTTTACCGGGCAGCAGTGGAACAGGTCCCAGTAGA ACCCTACAAGATCCCCTTGTCTCAGGCTGAAGTCATCCAGGAGGGCAGCGATGTGACTCTGGTTGCCTGG GGCACTCAGGTTCATGTCATCCGGGAGGTGGCTTCCATGGCCCAAGAAAAGCTTGGAGTATCTTGTGAAG TCATCGATCTGCGGACAATTGTGCCTTGGGATGTGGATACAGTTTGCAAGTCTGTGATCAAAACCGGGCG ACTGTTGATCAGCCACGAGGCTCCCTTAACAGGCGGCTTTGCCTCTGAGATCAGCTCCACGGTCCAGGAA GAATGTTTCTTGAACCTAGAGGCTCCAATATCTCGAGTTTGCGGATATGACACCCCGTTTCCTCACATCT TTGAGCCCTTTTATATCCCAGACAAATGGAAGTGCTACGATGCCCTTCGCAAGATGATCAACTATTGACG ACAGAGAAAACCAGGAAGATCATGACCAGACATGGAAATATTTTTCTGAAACCTTTTTATATTTCCTTAT ACTTCTTTCTTCTTACATATAGTTTTATGCAACGAACTGTCATGGATATTGGCTGATGAGCTGCGAATTA CTTATCATGTTAATATAAATGGATTGAGTGCACAGGAGGAATGCTTTAAGGAGTGCGTGGGCGGTGATCT TAAGACTGTTTCATGACTATAAATACCGGGTGGTGTTGTATTCAATAAAGCCAAATTACATCGCCTCAAA AAAAAAAAAAAAAAAAAAA
Restriction Sites EcoRI-NotI     
ACCN NM_199195
ORF Size 969 bp
Insert Size 969
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Reference Data
RefSeq BC064099, AAH64099
RefSeq Size 1419
RefSeq ORF 969
Locus ID 12040
Gene Summary This gene encodes the beta chain of the branched chain alpha ketoacid dehydrogenase (Bckdh) complex. The encoded protein exists in a heterotetrameric complex containing the Bckdh alpha subunit to form the E1 catalytic component of Bckdh complex. The Bckdh complex catalyzes the oxidative decarboxylation of branched chain ketoacids to their corresponding acyl-CoA esters, during the catabolism of leucine, isoleucine and valine. In humans, certain mutations in this gene cause maple syrup urine disease. Alternative splicing results in multiple transcript variants encoding different isoforms. A pseudogene for this gene has been identified. [provided by RefSeq, Apr 2015]
Transcript Variant: This variant (2) contains an alternate 5' terminal exon and uses a downstream start codon compared to variant 1. It encodes isoform 2 which has a shorter N-terminus compared to isoform 1.

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