A4galt (NM_001004150) Mouse Untagged Clone

CAT#: MC213078

A4galt (untagged) - Mouse alpha 1,4-galactosyltransferase (A4galt), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: A4galt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC213078 representing NM_001004150
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATCTCCTGTTCCCATCTGGAGGAGACCATGTCCAAGCCCCCCGACTGCCTGCTGCGGATGCTCA
  GGGGCACCCCCAGACAGCGGGTCTTCACCTTCTTCATCATCAGCTTCAAGTTCATGTTCTTGATCTCCAT
  CCTGATCTACTGGCACACTGTGGGTGCACCCAAGGACCAAAGAGAGTATAGCCTGCCAGTAGACTTCTCC
  TGCCCCCAGCTGGCCTTTCCTAGAGTCTCTGCCCCAGGCAACATCTTCTTCCTAGAGACATCGGACAGGA
  CCAGTCCCAACTTTCTATTTATGTGTTCTGTGGAATCAGCTGCCAGGGCACACCCTGAATCCCAGGTGGT
  TGTGCTGATGAAAGGGCTGCCTAGGGACACCACAGCTCAGCCCCGGAACCTCGGCATCTCTCTTCTGAGC
  TGTTTTCCTAATGTCTGGATACGACCTCTGGACTTGCAAGAACTGTTTGAGGACACACCACTGGCAGCCT
  GGTACTCGGAGGCACGGCACAGATGGGAGCCCTACCAGTTGCCCGTACTGTCTGATGCTTCCAGGATCGC
  ACTCCTCTGGAAGTTTGGTGGGATCTACCTGGACACAGACTTCATCGTCCTCAAGAACCTGCTCAACCTG
  ACCAACACGCTGGGCATCCAATCCCGCTATGTCCTCAACGGTGCCTTCCTAGCCTTCGAGCGCAAGCATG
  AGTTCTTGGCACTGTGTCTACATGACTTCGTGGCCAACTACAATGGTTGGATTTGGGGTCACCAGGGCCC
  CCAACTGCTTACCCGAGTCTTCAAGAAGTGGTGTTCCATCCAAAGCCTGGAAAAGAGCCATGCTTGCCGT
  GGGGTCACTGCCCTGCCCCCCGAGGCCTTCTACCCCATCCCCTGGCAGAACTGGAAGAAATACTTTGAAG
  ACATCAGCCCCGAGGAGCTGACCCAGCTGCTCAATGCCACCTATGCTGTTCATGTGTGGAACAAGAAAAG
  TCAGGGTACACACCTAGAGGCCACATCCAAGGCTCTGCTGGCCCAGCTACATGCCCGCTATTGCCCTACG
  ACACATAGAGCCATGAAGATGTACTTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001004150
• Insert Size: 1080 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004150.3, NP_001004150.1
• RefSeq Size: 2105 bp
• RefSeq ORF: 1080 bp
• Locus ID: 239559
• Cytogenetics: 15 E1
• Gene Summary: The protein encoded by this gene catalyzes the transfer of galactose to lactosylceramide to form globotriaosylceramide, which has been identified as the P(k) antigen of the P blood group system. The encoded protein, which is a type II membrane protein found in the Golgi, is also required for the synthesis of the bacterial verotoxins receptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All three variants encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.