A4galt (NM_001004150) Mouse Untagged Clone

CAT#: MC213078

A4galt (untagged) - Mouse alpha 1,4-galactosyltransferase (A4galt), transcript variant 2, (10ug)


  "NM_001004150" in other vectors (4)

Reconstitution Protocol

USD 420.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol A4galt
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC213078 representing NM_001004150
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCATCTCCTGTTCCCATCTGGAGGAGACCATGTCCAAGCCCCCCGACTGCCTGCTGCGGATGCTCA
GGGGCACCCCCAGACAGCGGGTCTTCACCTTCTTCATCATCAGCTTCAAGTTCATGTTCTTGATCTCCAT
CCTGATCTACTGGCACACTGTGGGTGCACCCAAGGACCAAAGAGAGTATAGCCTGCCAGTAGACTTCTCC
TGCCCCCAGCTGGCCTTTCCTAGAGTCTCTGCCCCAGGCAACATCTTCTTCCTAGAGACATCGGACAGGA
CCAGTCCCAACTTTCTATTTATGTGTTCTGTGGAATCAGCTGCCAGGGCACACCCTGAATCCCAGGTGGT
TGTGCTGATGAAAGGGCTGCCTAGGGACACCACAGCTCAGCCCCGGAACCTCGGCATCTCTCTTCTGAGC
TGTTTTCCTAATGTCTGGATACGACCTCTGGACTTGCAAGAACTGTTTGAGGACACACCACTGGCAGCCT
GGTACTCGGAGGCACGGCACAGATGGGAGCCCTACCAGTTGCCCGTACTGTCTGATGCTTCCAGGATCGC
ACTCCTCTGGAAGTTTGGTGGGATCTACCTGGACACAGACTTCATCGTCCTCAAGAACCTGCTCAACCTG
ACCAACACGCTGGGCATCCAATCCCGCTATGTCCTCAACGGTGCCTTCCTAGCCTTCGAGCGCAAGCATG
AGTTCTTGGCACTGTGTCTACATGACTTCGTGGCCAACTACAATGGTTGGATTTGGGGTCACCAGGGCCC
CCAACTGCTTACCCGAGTCTTCAAGAAGTGGTGTTCCATCCAAAGCCTGGAAAAGAGCCATGCTTGCCGT
GGGGTCACTGCCCTGCCCCCCGAGGCCTTCTACCCCATCCCCTGGCAGAACTGGAAGAAATACTTTGAAG
ACATCAGCCCCGAGGAGCTGACCCAGCTGCTCAATGCCACCTATGCTGTTCATGTGTGGAACAAGAAAAG
TCAGGGTACACACCTAGAGGCCACATCCAAGGCTCTGCTGGCCCAGCTACATGCCCGCTATTGCCCTACG
ACACATAGAGCCATGAAGATGTACTTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001004150
Insert Size 1080 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001004150.3, NP_001004150.1
RefSeq Size 2105 bp
RefSeq ORF 1080 bp
Locus ID 239559
Cytogenetics 15 E1
Gene Summary The protein encoded by this gene catalyzes the transfer of galactose to lactosylceramide to form globotriaosylceramide, which has been identified as the P(k) antigen of the P blood group system. The encoded protein, which is a type II membrane protein found in the Golgi, is also required for the synthesis of the bacterial verotoxins receptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010]
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All three variants encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.