Dkc1 (NM_001030307) Mouse Untagged Clone

CAT#: MC217214

Dkc1 (untagged) - Mouse dyskeratosis congenita 1, dyskerin homolog (human) (Dkc1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dkc1
• Synonyms: BC068171
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217214 representing NM_001030307
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATGCAGAAGTAATTACATTCCCAAAGAAGCATAAGAAGAAAAAAGACCGGAAGCCATTACAAG
  AAGATGATGTTGCCGAAATACAACATGCTGAAGAATTTCTTATCAAACCAGAATCTAAAGTGGCTCAATT
  AGACACTTCTCAGTGGCCCTTGTTACTAAAGAATTTTGATAAGCTAAATGTAAGGACAGCACACTATACA
  CCTCTTCCTTGTGGTTCAAATCCTCTGAAGAGGGAGATTGGGGACTATATCAGGACCGGTTTCATTAATC
  TTGACAAGCCCTCTAACCCCTCTTCCCATGAGGTGGTAGCCTGGATCCGACGAATACTTCGGGTAGAGAA
  GACTGGCCACAGTGGCACACTAGATCCCAAAGTGACTGGTTGTTTAATTGTGTGCATTGAACGAGCCACT
  CGTTTGGTGAAATCACAACAGAGTGCAGGCAAAGAGTATGTTGGAATTGTCCGGCTGCACAATGCTATTG
  AAGGGGGTACTCAGCTTTCTAGGGCCCTAGAAACTCTGACAGGTGCCCTGTTTCAGCGACCCCCACTTAT
  TGCTGCAGTAAAGAGGCAGCTTCGAGTGAGGACTATCTACGAGAGCAAAATGATAGAATATGATCCTGAG
  AGAAGATTAGGAATCTTTTGGGTAAGCTGTGAAGCTGGCACCTACATTCGGACACTATGCGTACACCTTG
  GCTTGTTACTGGGAGTTGGTGGTCAGATGCAGGAACTTCGGAGGGTGCGTTCTGGAGTCATGAGTGAAAA
  GGACCACATGGTGACAATGCATGATGTACTCGATGCTCAGTGGCTGTATGATAACCATAAGGATGAGAGT
  TACTTGCGGCGTGTTGTTTACCCTTTGGAAAAGCTGTTGACATCTCATAAACGTCTGGTTATGAAAGATA
  GTGCAGTGAATGCAATCTGCTATGGGGCCAAGATCATGCTTCCTGGTCTTCTTCGATATGAAGATGGCAT
  TGAGGTCAACCAGGAGATTGTGGTCATTACTACTAAGGGGGAAGCTATCTGCATGGCAATTGCATTGATG
  ACTACAGCAGTGATTTCTACCTGTGACCATGGTATAGTAGCCAAAATAAAGAGGGTGATCATGGAGAGAG
  ACACTTATCCTCGCAAGTGGGGTTTAGGTCCAAAGGCAAGTCAGAAGAAGATGATGATCAAGCAGGGCCT
  TCTGGACAAGCATGGCAAACCCACAGACAATACCCCTGCCACATGGAAACAGGATTACATTGACTATAGT
  GATTCTGGCAAGAACACACTGGTTACCGAAGCAGTACAAGCCCCTCAGTTAGCTGCTGAAGCAGTAAATG
  TCATAAAAAGGAAGCGGGATAGTGAGAGTGAAAGTGATGAGACCCCAACAGTTCCCCAGTTGAAGGAAAA
  GAAGAAGAAGAAGGATAAGAAGCCCAAAACTGTGCTAGAAAGTGGGGGCGAAACTGGAGATGGGGACAAC
  GACACCACCAAAAAGAAAAAGAAGAAGAAAGTAAAAGTGGTAGAAGAGATGTCTGAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001030307
• Insert Size: 1530 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001030307.2, NP_001025478.1
• RefSeq Size: 2803 bp
• RefSeq ORF: 1530 bp
• Locus ID: 245474
• Cytogenetics: X A7.3