Kpna4 (NM_008467) Mouse Untagged Clone

CAT#: MC217425

Kpna4 (untagged) - Mouse karyopherin (importin) alpha 4 (Kpna4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kpna4
• Synonyms: 1110058D08Rik; IPOA3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217425 representing NM_008467
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACAACGAGAAATTGGACAACCAACGGCTCAAGAATTTTAAGAACAAAGGCCGTGACTTGGAGA
  CTATGAGAAGACAACGAAATGAAGTTGTAGTTGAATTAAGGAAGAATAAAAGAGATGAACATCTCTTAAA
  GAGAAGGAATGTACCACAAGAAGATATCTGTGAAGACTCCGATATAGATGGTGATTATAGAGTGCAAAAT
  ACCTCTCTAGAAGCTATTGTTCAAAATGCTTCAAGTGATAACCAAGGAATTCAATTAAGTGCAGTTCAAG
  CTGCTAGGAAGCTTTTATCCAGTGATCGAAATCCACCAATTGATGACTTAATAAAATCTGGAATATTGCC
  TATTTTAGTTCATTGTCTTGAAAGAGATGACAATCCTTCTTTACAGTTTGAAGCTGCGTGGGCTTTGACA
  AACATTGCATCTGGAACGTCTGAACAAACGCAAGCAGTGGTTCAGTCCAATGCTGTGCCACTTTTCTTGA
  GGCTTCTGCATTCACCCCATCAGAATGTCTGTGAACAGGCAGTATGGGCGTTGGGAAACATCATAGGTGA
  TGGACCTCAGTGTAGAGATTATGTGATAAGTCTTGGAGTTGTAAAACCTCTGCTTTCATTCATAAGTCCA
  TCTATTCCTATAACATTCTTAAGAAATGTTACCTGGGTTATGGTCAATTTATGTCGCCACAAAGACCCAC
  CACCACCAATGGAGACAATTCAGGAGATTCTTCCAGCTCTTTGTGTATTAATTCATCACACAGATGTAAA
  TATCCTGGTAGATACTGTCTGGGCCCTGTCTTACCTTACGGATGCTGGCAATGAACAGATACAGATGGTA
  ATAGACTCTGGCATAGTTCCTCACTTGGTTCCGCTACTCAGCCACCAGGAAGTTAAAGTTCAGACTGCTG
  CACTGCGAGCTGTGGGCAACATTGTCACTGGAACTGATGAGCAAACACAAGTAGTTTTAAACTGTGATGC
  TCTCTCACACTTCCCAGCACTCCTCACTCATCCCAAAGAGAAAATTAATAAAGAAGCAGTGTGGTTCCTC
  TCCAACATCACTGCAGGAAATCAACAGCAGGTTCAGGCAGTAATTGATGCCAATCTTGTACCAATGATAA
  TACACCTTTTGGATAAGGGAGATTTTGGCACTCAGAAAGAAGCTGCCTGGGCCATAAGTAACTTAACAAT
  CAGCGGAAGGAAAGACCAAGTGGCCTATCTTATCCAACAAAATGTTATTCCACCTTTTTGCAATTTGCTG
  ACTGTAAAAGATGCACAAGTTGTGCAAGTAGTACTTGATGGACTAAGTAATATATTAAAAATGGCTGAAG
  ATCAAGCAGAAACCATAGCCAATCTCATAGAAGAATGTGGTGGACTGGAGAAAATTGAACAACTTCAGAA
  TCATGAAAATGAGGACATCTACAAATTGGCCTATGAGATCATTGACCAGTTCTTCTCTTCAGATGATATT
  GATGAAGATCCTAGCCTTGTTCCAGAGTCAGTTCAAGGTGGAACATTTGGTTTCAATTCATCGACCAATG
  TACCAACAGAGGGGTTCCAGTTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008467
• ORF Size: 1566 bp
• Insert Size: 1566
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_008467.4, NP_032493.1
• RefSeq Size: 3726
• RefSeq ORF: 1566
• Locus ID: 16649
• Gene Summary: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. [UniProtKB/Swiss-Prot Function]