Ryk (NM_013649) Mouse Untagged Clone

CAT#: MC219398

Ryk (untagged) - Mouse receptor-like tyrosine kinase (Ryk), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ryk
• Synonyms: AW536699; ERK-3; Vik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219398 representing NM_013649.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCGCGCGGGCCGGGGCGGCGTCCCGGGGAGCGGCGGCCTGAGGGCCCCGCCGCCGCCGCTGCTGCTG
  CTGCTGCTGGCGATGCTGCCCGCCGCCGCCCCGCGGTCCCCGGCCCTGGCCGCCGCTCCTGCGGGACCC
  AGCGTGAGCCTCTACCTGAGCGAGGACGAGGTGCGCCGGCTGCTTGGTCTTGATGCAGAGCTTTACTAT
  GTGAGAAATGACCTCATCAGTCACTACGCTCTGTCCTTTAACCTGCTAGTGCCCAGTGAGACAAACTTC
  CTGCACTTCACTTGGCATGCAAAGTCCAAGGTTGAATATAAGCTGGGATTCCAAGTAGACAACTTTGTG
  GCTATGGGCATGCCCCAGGTCAATATTTCTGCTCAAGGGGAGGTCCCACGCACTTTATCAGTGTTTCGG
  GTCGAGCTTTCTTGTACCGGCAAAGTCGACTCTGAAGTCATGATTCTAATGCAGCTCAATCTGACAGTG
  AATTCCTCAAAAAATTTTACAGTTTTAAATTTTAAACGAAGGAAAATGTGCTACAAAAAACTTGAAGAA
  GTAAAAACTTCAGCCTTGGACAAAAACACTAGCAGAACTATTTATGACCCTGTCCATGCAGCGCCAACG
  ACTTCCACGCGTGTGTTTTACATCAGTGTAGGGGTTTGCTGTGCAGTGATATTTCTTGTAGCAATAATA
  TTAGCCGTTTTGCACCTTCATAGCATGAAAAGGATTGAACTGGATGACAGCATCAGCGCCAGCAGTAGT
  TCCCAGGGGCTGTCTCAGCCGTCTACCCAGACGACCCAGTATCTGAGAGCTGACACACCCAACAATGCA
  ACGCCTATCACCAGCTCCTCAGGTTATCCTACCTTGCGGATAGAGAAGAACGACTTGCGAAGTGTCACT
  CTTCTGGAAGCCAAAGCCAAGGTGAAGGATATCGCAATATCCAGAGAAAGGATCACACTGAAAGATGTC
  CTCCAAGAAGGTACTTTTGGGCGTATTTTCCATGGGATTTTAGTAGATGAAAAAGATCCAAATAAAGAG
  AAGCAAACATTTGTAAAAACAGTTAAAGACCAAGCATCTGAAGTTCAGGTGACGATGATGCTCACCGAG
  AGCTGCAAGCTTCGAGGTCTGCACCACAGAAACCTCCTTCCTATTACTCATGTGTGCATAGAAGAAGGA
  GAAAAGCCCATGGTGGTATTGCCATACATGAATTGGGGGAATCTTAAATTATTTCTTCGGCAGTGCAAA
  TTAGTAGAAGCCAATAATCCACAGGCAATTTCCCAGCAAGATCTGGTCCATATGGCTATTCAGATTGCC
  TGCGGGATGAGCTACCTGGCGAGGAGAGAAGTGATCCATAGAGACCTGGCTGCTAGGAACTGTGTCATC
  GACGACACTCTTCAAGTCAAGATCACAGACAATGCCCTTTCCAGAGACTTGTTTCCTATGGACTACCAC
  TGCCTAGGGGACAACGAGAACAGGCCAGTGAGATGGATGGCTCTGGAAAGTCTGGTTAATAATGAGTTC
  TCTAGTGCTAGTGACGTGTGGGCCTTTGGAGTGACGCTGTGGGAGCTCATGACTCTGGGCCAGACGCCC
  TACGTGGACATCGACCCCTTTGAGATGGCCGCTTACCTGAAAGATGGTTACCGAATAGCCCAGCCAATC
  AACTGCCCTGATGAACTGTTTGCTGTGATGGCCTGTTGCTGGGCCTTGGACCCTGAGGAGAGGCCTAAG
  TTCCAGCAGCTGGTCCAGTGCCTCACAGAGTTCCACGCTGCCCTGGGAGCCTACGTCTGA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
• Restriction Sites: SgfI-RsrII Plasmid Map
• ACCN: NM_013649
• Insert Size: 1785 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013649.3
• RefSeq Size: 3267 bp
• RefSeq ORF: 1785 bp
• Locus ID: 20187
• UniProt ID: Q01887
• Cytogenetics: 9 54.72 cM
• MW: 66.3 kDa
• Gene Summary: May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).