Dnmt1 (BC053047) Mouse Untagged Clone

CAT#: MC224567

Dnmt1 (untagged) - Mouse DNA methyltransferase (cytosine-5) 1 (cDNA clone MGC:62302 IMAGE:5709259), (10ug)


  "BC053047" in other vectors (4)

Reconstitution Protocol

USD 2,020.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Dnmt1
Synonyms Dnmt, MTase, Dnmt1o
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC053047
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAGCGCGAACAGCTCCAGCCCGAGTGCCTGCGCTTGCCTCCCCGGCAGGCTCGCTCCCGGACCATG
TCCGCAGGCGGCTCAAAGACTTGGAAAGAGATGGCTTAACAGAAAAGGAGTGTGTGAGGGAGAAATTAAA
CTTACTGCATGAATTCCTGCAAACAGAAATAAAAAGCCAGTTGTGTGACTTGGAAACCAAATTACATAAA
GAGGAATTATCTGAGGAAGGCTACCTGGCTAAAGTCAAGTCCCTCTTAAATAAGGATTTGTCCTTGGAGA
ACGGAACACACACTCTCACTCAAAAAGCCAACGGTTGTCCCGCCAACGGGAGCCGGCCAACCTGGAGAGC
AGAAATGGCAGACTCAAATAGATCCCCAAGATCCAGGCCCAAGCCTCGGGGACCCAGGAGAAGCAAGTCG
GACAGTGACACCCTTTGTAAGGACACGAGACATACTGCAGTTGAAACTTCACCTAGTTCCGTGGCTACGA
GGAGAACCACCAGGCAGACCACCATCACGGCTCACTTCACGAAGGGCCCCACTAAACGGAAACCCAAGGA
AGAGTCGGAAGAGGGGAACTCGGCTGAGTCGGCTGCAGAGGAGAGAGACCAGGATAAGAAACGCAGAGTT
GTAGACACAGAGAGTGGTGCTGCAGCTGCTGTGGAGAAACTGGAAGAGGTAACAGCGGGAACCCAGCTGG
GTCCGGAAGAGCCATGTGAACAGGAAGATGACAACAGGAGTCTTCGACGTCACACCAGAGAGCTATCATT
GAGGCGGAAATCAAAGGAGGATCCAGACAGAGAAGCAAGACCGGAAACTCACTTGGACGAGGACGAGGAC
GGAAAAAAGGATAAAAGAAGTTCCAGACCCAGGAGCCAGCCCAGAGATCCAGCTGCCAAACGGAGACCCA
AGGAAGCAGAGCCAGAGCAGGTAGCTCCAGAGACTCCCGAGGACAGAGACGAGGATGAGAGGGAGGAGAA
GAGACGAAAAACGACACGTAAAAAACTGGAGTCACACACCGTTCCCGTTCAGAGCAGATCGGAGAGAAAA
GCCGCTCAAAGCAAAAGTGTGATCCCGAAGATCAACTCACCAAAGTGCCCCGAGTGTGGCCAGCACCTAG
ACGACCCTAACCTGAAGTACCAGCAGCACCCTGAGGATGCTGTGGATGAACCCCAGATGTTGACCAGTGA
GAAACTGTCCATCTACGACTCCACCTCGACCTGGTTTGATACTTATGAAGATTCTCCCATGCATAGGTTC
ACTTCCTTCAGTGTGTACTGCAGTCGCGGGCACCTGTGTCCTGTCGACACCGGTCTCATTGAGAAGAATG
TAGAGCTCTACTTTTCTGGGTGTGCCAAAGCAATTCATGACGAGAATCCATCTATGGAAGGTGGTATTAA
TGGCAAAAACCTCGGGCCAATCAATCAGTGGTGGCTCAGTGGCTTTGATGGTGGCGAGAAGGTGCTCATT
GGCTTCTCCACTGCATTTGCTGAATACATTTTGATGGAGCCCAGCAAAGAGTATGAGCCAATATTTGGGC
TGATGCAGGAGAAAATTTACATCAGCAAGATTGTTGTTGAGTTCCTGCAAAACAATCCTGATGCTGTATA
TGAAGACCTGATCAATAAGATTGAGACCACTGTTCCTCCTTCTACCATTAATGTGAACCGGTTCACAGAG
GACTCCCTCTTACGCCACGCCCAGTTTGTAGTGAGCCAGGTAGAGAGTTACGACGAAGCCAAGGACGATG
ATGAGACCCCCATCTTCTTGTCTCCCTGTATGAGAGCCCTGATCCATTTGGCTGGTGTCTCCCTGGGACA
GAGGCGAGCAACAAGGCGCGTCATGGGTGCTACCAAGGAGAAGGACAAAGCACCCACGAAAGCCACCACC
ACCAAGCTGGTCTATCAGATCTTTGACACTTTCTTCTCAGAGCAGATTGAGAAGTATGATAAGGAGGACA
AGGAGAATGCCATGAAGCGCCGCCGCTGTGGTGTCTGTGAGGTCTGTCAGCAGCCTGAGTGTGGGAAGTG
CAAGGCGTGCAAAGATATGGTGAAGTTTGGTGGCACTGGACGGAGTAAGCAGGCTTGCCTCAAGAGGAGG
TGTCCTAACTTGGCGGTGAAGGAGGCAGACGACGATGAAGAGGCTGATGATGATGTGTCAGAGATGCCAT
CACCCAAAAAGCTGCATCAGGGGAAGAAGAAGAAGCAGAACAAGGACCGCATCTCCTGGCTTGGGCAGCC
TATGAAGATTGAAGAGAATAGAACTTACTATCAGAAGGTGAGCATCGATGAGGAGATGCTAGAGGTGGGC
GACTGCGTCTCGGTCATTCCAGATGATTCCTCCAAACCACTCTATCTAGCCAGGGTCACAGCTCTGTGGG
AAGACAAAAATGGTCAGATGATGTTCCATGCGCACTGGTTCTGCGCTGGGACAGACACAGTCCTGGGAGC
CACCTCCGACCCCCTGGAACTGTTCCTGGTGGGCGAGTGCGAAAACATGCAGCTTTCCTACATCCACAGC
AAGGTCAAGGTCATCTACAAAGCCCCTTCTGAAAACTGGGCCATGGAGGGAGGCACAGACCCTGAGACCA
CACTGCCTGGGGCTGAGGATGGCAAGACTTACTTCTTCCAGCTCTGGTACAACCAGGAGTACGCAAGGTT
TGAATCCCCACCCAAGACCCAGCCGACCGAGGACAACAAGCACAAGTTCTGCCTATCTTGTATCCGGCTG
GCTGAGCTGAGACAAAAAGAAATGCCCAAGGTCCTGGAACAAATTGAGGAGGTGGATGGCCGGGTCTACT
GCAGTTCCATCACCAAGAATGGTGTTGTCTACCGACTGGGTGACAGTGTGTACCTTCCTCCCGAGGCCTT
TACTTTCAACATCAAAGTGGCTAGCCCCGTGAAACGCCCAAAGAAGGATCCTGTGAACGAGACCCTGTAC
CCTGAGCACTACCGCAAGTATTCTGACTACATCAAGGGGAGCAACCTGGATGCTCCAGAGCCCTATCGCA
TCGGTCGGATAAAAGAGATCCACTGTGGCAAGAAGAAAGGCAAGGTCAACGAGGCAGACATCAAGCTGAG
GCTCTACAAGTTCTACAGGCCTGAGAATACCCACAGGTCCTACAACGGATCCTATCACACTGACATCAAC
ATGCTTTACTGGAGCGACGAGGAAGCTGTGGTGAACTTCAGCGACGTGCAGGGCCGCTGTACCGTGGAGT
ACGGGGAAGACCTACTTGAGAGCATCCAGGATTATTCACAAGGGGGCCCTGACCGCTTCTACTTCCTCGA
GGCCTACAATTCAAAGACCAAGAACTTTGAAGACCCACCAAACCATGCCCGCAGCCCTGGGAACAAAGGG
AAAGGGAAGGGGAAAGGGAAGGGGAAGGGGAAGCATCAGGTGTCAGAGCCCAAAGAGCCTGAGGCAGCCA
TCAAACTGCCCAAGCTCCGGACCCTGGATGTGTTTTCCGGCTGTGGAGGGTTATCGGAAGGATTCCACCA
AGCAGGCATCTCGGAAACGCTGTGGGCCATCGAGATGTGGGACCCGGCAGCCCAGGCATTTCGGCTGAAC
AACCCCGGCACCACAGTGTTCACAGAGGACTGCAACGTGCTTCTTAAGCTGGTCATGGCTGGGGAGGTGA
CCAACTCTCTGGGCCAAAGGCTGCCACAGAAGGGCGATGTGGAGATGCTGTGTGGTGGGCCACCCTGCCA
GGGCTTCAGTGGCATGAACCGCTTCAACTCCCGCACTTACTCCAAGTTCAAAAACTCCCTAGTGGTCTCC
TTCCTCAGCTACTGTGACTACTACCGGCCTCGGTTCTTCCTTCTGGAGAACGTCAGGAACTTCGTGTCCT
ACAGACGCTCCATGGTGCTGAAGCTCACACTGCGCTGCCTGGTCCGCATGGGCTACCAGTGCACCTTTGG
TGTGCTCCAGGCTGGACAGTATGGCGTGGCCCAGACACGAAGGAGGGCCATCATCTTGGCTGCAGCCCCA
GGAGAAAAGCTGCCTCTGTTCCCAGAGCCTCTGCATGTGTTTGCGCCCCGTGCCTGCCAGCTGAGCGTTG
TGGTGGATGACAAGAAGTTTGTTAGCAACATAACGAGGCTGAGCTCGGGGCCCTTCCGAACCATCACCGT
GCGAGACACCATGTCTGACCTCCCCGAGATCCAGAATGGAGCCTCGAATTCTGAGATCCCCTACAATGGA
GAGCCACTGTCCTGGTTCCAGAGGCAGCTGCGAGGATCACACTACCAGCCCATCCTCAGGGACCATATCT
GCAAGGACATGAGCCCACTGGTGGCTGCCCGCATGCGGCACATCCCACTGTTCCCAGGATCAGATTGGCG
TGACCTGCCCAACATACAGGTGCGGCTGGGAGATGGCGTCATAGCCCATAAGCTACAGTACACCTTTCAT
GATGTGAAAAATGGCTACAGCAGCACCGGTGCCCTGCGTGGAGTCTGTTCCTGTGCAGAAGGCAAGGCCT
GCGACCCTGAGTCCAGGCAATTCAGCACCCTCATCCCCTGGTGCCTGCCGCACACTGGGAACCGGCACAA
CCACTGGGCTGGCCTCTACGGGCGTCTGGAGTGGGATGGCTTCTTCAGCACCACTGTCACCAACCCTGAG
CCCATGGGCAAGCAGGGTCGGGTGCTCCACCCGGAGCAGCACCGGGTCGTGAGTGTTCGGGAATGTGCCC
GCTCCCAGGGCTTTCCAGATAGCTACCGGTTCTTCGGCAACATCCTGGACAGACACCGGCAGGTGGGTAA
TGCTGTGCCACCACCCCTGGCCAAAGCCATTGGCCTGGAGATTAAGCTCTGCCTGCTGTCCAGTGCTCGG
GAGAGCGCATCAGCTGCAGTTAAAGCAAAAGAGGAGGCTGCTACCAAGGACTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Restriction Sites SgfI-MluI     
ACCN BC053047
ORF Size 4884 bp
Insert Size 4884
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Reference Data
RefSeq BC053047, AAH53047
RefSeq Size 5249
RefSeq ORF 4883
Locus ID 13433
Gene Summary This gene encodes a methyltransferase that preferentially methylates cytosines of CpG residues in hemimethylated DNA to generate fully methylated CpG base pairs during DNA replication. This enzyme plays roles in diverse cellular processes including cell cycle regulation, DNA repair, and telomere maintenance. The encoded protein is composed of an N-terminal domain with a nuclear localization sequence and replication fork-targeting domain, a DNA-binding CXXC domain, two bromo-adjacent homology domains, and a C-terminal catalytic domain. Mouse embryonic stem cells mutant for this gene are viable, but when introduced into the germ line, cause a recessive lethal phenotype with mutant embryos displaying stunted growth and developmental defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

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