Dnmt1 (BC053047) Mouse Tagged ORF Clone

CAT#: MR212057

Dnmt1 (Myc-DDK-tagged) - Mouse DNA methyltransferase (cytosine-5) 1 (cDNA clone MGC:62302 IMAGE:5709259)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dnmt1
• Synonyms: Dnmt, MTase, Dnmt1o
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212057 representing BC053047
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCGCGAACAGCTCCAGCCCGAGTGCCTGCGCTTGCCTCCCCGGCAGGCTCGCTCCCGGACCATG
  TCCGCAGGCGGCTCAAAGACTTGGAAAGAGATGGCTTAACAGAAAAGGAGTGTGTGAGGGAGAAATTAAA
  CTTACTGCATGAATTCCTGCAAACAGAAATAAAAAGCCAGTTGTGTGACTTGGAAACCAAATTACATAAA
  GAGGAATTATCTGAGGAAGGCTACCTGGCTAAAGTCAAGTCCCTCTTAAATAAGGATTTGTCCTTGGAGA
  ACGGAACACACACTCTCACTCAAAAAGCCAACGGTTGTCCCGCCAACGGGAGCCGGCCAACCTGGAGAGC
  AGAAATGGCAGACTCAAATAGATCCCCAAGATCCAGGCCCAAGCCTCGGGGACCCAGGAGAAGCAAGTCG
  GACAGTGACACCCTTTGTAAGGACACGAGACATACTGCAGTTGAAACTTCACCTAGTTCCGTGGCTACGA
  GGAGAACCACCAGGCAGACCACCATCACGGCTCACTTCACGAAGGGCCCCACTAAACGGAAACCCAAGGA
  AGAGTCGGAAGAGGGGAACTCGGCTGAGTCGGCTGCAGAGGAGAGAGACCAGGATAAGAAACGCAGAGTT
  GTAGACACAGAGAGTGGTGCTGCAGCTGCTGTGGAGAAACTGGAAGAGGTAACAGCGGGAACCCAGCTGG
  GTCCGGAAGAGCCATGTGAACAGGAAGATGACAACAGGAGTCTTCGACGTCACACCAGAGAGCTATCATT
  GAGGCGGAAATCAAAGGAGGATCCAGACAGAGAAGCAAGACCGGAAACTCACTTGGACGAGGACGAGGAC
  GGAAAAAAGGATAAAAGAAGTTCCAGACCCAGGAGCCAGCCCAGAGATCCAGCTGCCAAACGGAGACCCA
  AGGAAGCAGAGCCAGAGCAGGTAGCTCCAGAGACTCCCGAGGACAGAGACGAGGATGAGAGGGAGGAGAA
  GAGACGAAAAACGACACGTAAAAAACTGGAGTCACACACCGTTCCCGTTCAGAGCAGATCGGAGAGAAAA
  GCCGCTCAAAGCAAAAGTGTGATCCCGAAGATCAACTCACCAAAGTGCCCCGAGTGTGGCCAGCACCTAG
  ACGACCCTAACCTGAAGTACCAGCAGCACCCTGAGGATGCTGTGGATGAACCCCAGATGTTGACCAGTGA
  GAAACTGTCCATCTACGACTCCACCTCGACCTGGTTTGATACTTATGAAGATTCTCCCATGCATAGGTTC
  ACTTCCTTCAGTGTGTACTGCAGTCGCGGGCACCTGTGTCCTGTCGACACCGGTCTCATTGAGAAGAATG
  TAGAGCTCTACTTTTCTGGGTGTGCCAAAGCAATTCATGACGAGAATCCATCTATGGAAGGTGGTATTAA
  TGGCAAAAACCTCGGGCCAATCAATCAGTGGTGGCTCAGTGGCTTTGATGGTGGCGAGAAGGTGCTCATT
  GGCTTCTCCACTGCATTTGCTGAATACATTTTGATGGAGCCCAGCAAAGAGTATGAGCCAATATTTGGGC
  TGATGCAGGAGAAAATTTACATCAGCAAGATTGTTGTTGAGTTCCTGCAAAACAATCCTGATGCTGTATA
  TGAAGACCTGATCAATAAGATTGAGACCACTGTTCCTCCTTCTACCATTAATGTGAACCGGTTCACAGAG
  GACTCCCTCTTACGCCACGCCCAGTTTGTAGTGAGCCAGGTAGAGAGTTACGACGAAGCCAAGGACGATG
  ATGAGACCCCCATCTTCTTGTCTCCCTGTATGAGAGCCCTGATCCATTTGGCTGGTGTCTCCCTGGGACA
  GAGGCGAGCAACAAGGCGCGTCATGGGTGCTACCAAGGAGAAGGACAAAGCACCCACGAAAGCCACCACC
  ACCAAGCTGGTCTATCAGATCTTTGACACTTTCTTCTCAGAGCAGATTGAGAAGTATGATAAGGAGGACA
  AGGAGAATGCCATGAAGCGCCGCCGCTGTGGTGTCTGTGAGGTCTGTCAGCAGCCTGAGTGTGGGAAGTG
  CAAGGCGTGCAAAGATATGGTGAAGTTTGGTGGCACTGGACGGAGTAAGCAGGCTTGCCTCAAGAGGAGG
  TGTCCTAACTTGGCGGTGAAGGAGGCAGACGACGATGAAGAGGCTGATGATGATGTGTCAGAGATGCCAT
  CACCCAAAAAGCTGCATCAGGGGAAGAAGAAGAAGCAGAACAAGGACCGCATCTCCTGGCTTGGGCAGCC
  TATGAAGATTGAAGAGAATAGAACTTACTATCAGAAGGTGAGCATCGATGAGGAGATGCTAGAGGTGGGC
  GACTGCGTCTCGGTCATTCCAGATGATTCCTCCAAACCACTCTATCTAGCCAGGGTCACAGCTCTGTGGG
  AAGACAAAAATGGTCAGATGATGTTCCATGCGCACTGGTTCTGCGCTGGGACAGACACAGTCCTGGGAGC
  CACCTCCGACCCCCTGGAACTGTTCCTGGTGGGCGAGTGCGAAAACATGCAGCTTTCCTACATCCACAGC
  AAGGTCAAGGTCATCTACAAAGCCCCTTCTGAAAACTGGGCCATGGAGGGAGGCACAGACCCTGAGACCA
  CACTGCCTGGGGCTGAGGATGGCAAGACTTACTTCTTCCAGCTCTGGTACAACCAGGAGTACGCAAGGTT
  TGAATCCCCACCCAAGACCCAGCCGACCGAGGACAACAAGCACAAGTTCTGCCTATCTTGTATCCGGCTG
  GCTGAGCTGAGACAAAAAGAAATGCCCAAGGTCCTGGAACAAATTGAGGAGGTGGATGGCCGGGTCTACT
  GCAGTTCCATCACCAAGAATGGTGTTGTCTACCGACTGGGTGACAGTGTGTACCTTCCTCCCGAGGCCTT
  TACTTTCAACATCAAAGTGGCTAGCCCCGTGAAACGCCCAAAGAAGGATCCTGTGAACGAGACCCTGTAC
  CCTGAGCACTACCGCAAGTATTCTGACTACATCAAGGGGAGCAACCTGGATGCTCCAGAGCCCTATCGCA
  TCGGTCGGATAAAAGAGATCCACTGTGGCAAGAAGAAAGGCAAGGTCAACGAGGCAGACATCAAGCTGAG
  GCTCTACAAGTTCTACAGGCCTGAGAATACCCACAGGTCCTACAACGGATCCTATCACACTGACATCAAC
  ATGCTTTACTGGAGCGACGAGGAAGCTGTGGTGAACTTCAGCGACGTGCAGGGCCGCTGTACCGTGGAGT
  ACGGGGAAGACCTACTTGAGAGCATCCAGGATTATTCACAAGGGGGCCCTGACCGCTTCTACTTCCTCGA
  GGCCTACAATTCAAAGACCAAGAACTTTGAAGACCCACCAAACCATGCCCGCAGCCCTGGGAACAAAGGG
  AAAGGGAAGGGGAAAGGGAAGGGGAAGGGGAAGCATCAGGTGTCAGAGCCCAAAGAGCCTGAGGCAGCCA
  TCAAACTGCCCAAGCTCCGGACCCTGGATGTGTTTTCCGGCTGTGGAGGGTTATCGGAAGGATTCCACCA
  AGCAGGCATCTCGGAAACGCTGTGGGCCATCGAGATGTGGGACCCGGCAGCCCAGGCATTTCGGCTGAAC
  AACCCCGGCACCACAGTGTTCACAGAGGACTGCAACGTGCTTCTTAAGCTGGTCATGGCTGGGGAGGTGA
  CCAACTCTCTGGGCCAAAGGCTGCCACAGAAGGGCGATGTGGAGATGCTGTGTGGTGGGCCACCCTGCCA
  GGGCTTCAGTGGCATGAACCGCTTCAACTCCCGCACTTACTCCAAGTTCAAAAACTCCCTAGTGGTCTCC
  TTCCTCAGCTACTGTGACTACTACCGGCCTCGGTTCTTCCTTCTGGAGAACGTCAGGAACTTCGTGTCCT
  ACAGACGCTCCATGGTGCTGAAGCTCACACTGCGCTGCCTGGTCCGCATGGGCTACCAGTGCACCTTTGG
  TGTGCTCCAGGCTGGACAGTATGGCGTGGCCCAGACACGAAGGAGGGCCATCATCTTGGCTGCAGCCCCA
  GGAGAAAAGCTGCCTCTGTTCCCAGAGCCTCTGCATGTGTTTGCGCCCCGTGCCTGCCAGCTGAGCGTTG
  TGGTGGATGACAAGAAGTTTGTTAGCAACATAACGAGGCTGAGCTCGGGGCCCTTCCGAACCATCACCGT
  GCGAGACACCATGTCTGACCTCCCCGAGATCCAGAATGGAGCCTCGAATTCTGAGATCCCCTACAATGGA
  GAGCCACTGTCCTGGTTCCAGAGGCAGCTGCGAGGATCACACTACCAGCCCATCCTCAGGGACCATATCT
  GCAAGGACATGAGCCCACTGGTGGCTGCCCGCATGCGGCACATCCCACTGTTCCCAGGATCAGATTGGCG
  TGACCTGCCCAACATACAGGTGCGGCTGGGAGATGGCGTCATAGCCCATAAGCTACAGTACACCTTTCAT
  GATGTGAAAAATGGCTACAGCAGCACCGGTGCCCTGCGTGGAGTCTGTTCCTGTGCAGAAGGCAAGGCCT
  GCGACCCTGAGTCCAGGCAATTCAGCACCCTCATCCCCTGGTGCCTGCCGCACACTGGGAACCGGCACAA
  CCACTGGGCTGGCCTCTACGGGCGTCTGGAGTGGGATGGCTTCTTCAGCACCACTGTCACCAACCCTGAG
  CCCATGGGCAAGCAGGGTCGGGTGCTCCACCCGGAGCAGCACCGGGTCGTGAGTGTTCGGGAATGTGCCC
  GCTCCCAGGGCTTTCCAGATAGCTACCGGTTCTTCGGCAACATCCTGGACAGACACCGGCAGGTGGGTAA
  TGCTGTGCCACCACCCCTGGCCAAAGCCATTGGCCTGGAGATTAAGCTCTGCCTGCTGTCCAGTGCTCGG
  GAGAGCGCATCAGCTGCAGTTAAAGCAAAAGAGGAGGCTGCTACCAAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212057 representing BC053047
  Red=Cloning site Green=Tags(s)
  
  MPARTAPARVPALASPAGSLPDHVRRRLKDLERDGLTEKECVREKLNLLHEFLQTEIKSQLCDLETKLHK
  EELSEEGYLAKVKSLLNKDLSLENGTHTLTQKANGCPANGSRPTWRAEMADSNRSPRSRPKPRGPRRSKS
  DSDTLCKDTRHTAVETSPSSVATRRTTRQTTITAHFTKGPTKRKPKEESEEGNSAESAAEERDQDKKRRV
  VDTESGAAAAVEKLEEVTAGTQLGPEEPCEQEDDNRSLRRHTRELSLRRKSKEDPDREARPETHLDEDED
  GKKDKRSSRPRSQPRDPAAKRRPKEAEPEQVAPETPEDRDEDEREEKRRKTTRKKLESHTVPVQSRSERK
  AAQSKSVIPKINSPKCPECGQHLDDPNLKYQQHPEDAVDEPQMLTSEKLSIYDSTSTWFDTYEDSPMHRF
  TSFSVYCSRGHLCPVDTGLIEKNVELYFSGCAKAIHDENPSMEGGINGKNLGPINQWWLSGFDGGEKVLI
  GFSTAFAEYILMEPSKEYEPIFGLMQEKIYISKIVVEFLQNNPDAVYEDLINKIETTVPPSTINVNRFTE
  DSLLRHAQFVVSQVESYDEAKDDDETPIFLSPCMRALIHLAGVSLGQRRATRRVMGATKEKDKAPTKATT
  TKLVYQIFDTFFSEQIEKYDKEDKENAMKRRRCGVCEVCQQPECGKCKACKDMVKFGGTGRSKQACLKRR
  CPNLAVKEADDDEEADDDVSEMPSPKKLHQGKKKKQNKDRISWLGQPMKIEENRTYYQKVSIDEEMLEVG
  DCVSVIPDDSSKPLYLARVTALWEDKNGQMMFHAHWFCAGTDTVLGATSDPLELFLVGECENMQLSYIHS
  KVKVIYKAPSENWAMEGGTDPETTLPGAEDGKTYFFQLWYNQEYARFESPPKTQPTEDNKHKFCLSCIRL
  AELRQKEMPKVLEQIEEVDGRVYCSSITKNGVVYRLGDSVYLPPEAFTFNIKVASPVKRPKKDPVNETLY
  PEHYRKYSDYIKGSNLDAPEPYRIGRIKEIHCGKKKGKVNEADIKLRLYKFYRPENTHRSYNGSYHTDIN
  MLYWSDEEAVVNFSDVQGRCTVEYGEDLLESIQDYSQGGPDRFYFLEAYNSKTKNFEDPPNHARSPGNKG
  KGKGKGKGKGKHQVSEPKEPEAAIKLPKLRTLDVFSGCGGLSEGFHQAGISETLWAIEMWDPAAQAFRLN
  NPGTTVFTEDCNVLLKLVMAGEVTNSLGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVS
  FLSYCDYYRPRFFLLENVRNFVSYRRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAP
  GEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEIQNGASNSEIPYNG
  EPLSWFQRQLRGSHYQPILRDHICKDMSPLVAARMRHIPLFPGSDWRDLPNIQVRLGDGVIAHKLQYTFH
  DVKNGYSSTGALRGVCSCAEGKACDPESRQFSTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPE
  PMGKQGRVLHPEQHRVVSVRECARSQGFPDSYRFFGNILDRHRQVGNAVPPPLAKAIGLEIKLCLLSSAR
  ESASAAVKAKEEAATKD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC053047
• ORF Size: 4881 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC053047.1
• RefSeq Size: 5249
• RefSeq ORF: 4883
• Locus ID: 13433
• MW: 184 kDa
• Gene Summary: This gene encodes a methyltransferase that preferentially methylates cytosines of CpG residues in hemimethylated DNA to generate fully methylated CpG base pairs during DNA replication. This enzyme plays roles in diverse cellular processes including cell cycle regulation, DNA repair, and telomere maintenance. The encoded protein is composed of an N-terminal domain with a nuclear localization sequence and replication fork-targeting domain, a DNA-binding CXXC domain, two bromo-adjacent homology domains, and a C-terminal catalytic domain. Mouse embryonic stem cells mutant for this gene are viable, but when introduced into the germ line, cause a recessive lethal phenotype with mutant embryos displaying stunted growth and developmental defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]