Mdc1 (NM_001010833) Mouse Untagged Clone

CAT#: MC224851

Mdc1 (untagged) - Mouse mediator of DNA damage checkpoint 1 (Mdc1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mdc1
• Synonyms: 6820401C03; AA413496; mKIAA0170; Nfbd1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224851 representing NM_001010833
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAGCACCCAGGTGATTGACTGGGATGCTGAAGAAGAGGAAGAGACAGAACTATCCAGTGGCTCCT
  TGGGGTATAGTGTGGAGCCTATAGGGCAGCTACGTCTCTTCAGTGGAACTCATGGACCAGAAAGAGATTT
  CCCCCTCTACCTTGGCAAGAATGTAGTTGGTCGAAGCCCTGACTGCTCTGTGGCCCTGCCTTTTCCATCC
  ATCTCCAAACAGCATGCAGTAATTGAAATCTCAGCTTGGAACAAAGCCCCTATCCTCCAGGATTGTGGGA
  GCCTCAATGGCACTCAAATCGTAAAGCCTCCTAGGGTCCTACCTCCTGGAGTGAGCCATCGTCTGAGGGA
  CCAGGAATTAATTCTGTTTGCAGATTTTCCCTGCCAGTACCATCGCCTGGATGTCCCACCACCCTTGGTC
  CCTCGGAGTCTTCTAACTATAGAGAAAACCCCCAGAATACGGATAGAATCCCAAAATTCCAGAGTTTTGT
  TGGCTGCCGATTCAGAGGAAGAAGGGGATTTTCCTTCTGGAAGGTGTGTGGCGAATGGACAAAGGAATAC
  AGCATCCCCTTCAGCAACAGTAGTTCCAGAAAGTGATGAAGAGGTCTCTTCTCCGGCCCCAAGTGTCCCC
  GGGCCATCTTCACCCTTTGGTTTGGGCAGTGACACTGATGAAGAACAAGGTCAGCAACCAGGAGTAGAGG
  AGTCCTCTTTAGCTGACAGCAGTGGTGCTGCAGGGGAAGCTGAGCAGCCTGAAGCTAACGGAACGACAGC
  TGGCATACAGGCTCAGCCTACTGAGCACAAATTGAAGGACACAAAAGTCAAGAAGGAGGCAGGCAGGGCA
  GGGGTCTCAGATGGGTCAGTTCTGGAGAGGAGCCCCACTCTTGGAGAGGACAGTGACACAGAAGTGGACG
  AAGATCACAAACCTGGGTTTGCGGACAGTGAGACAGATGTGGAAGAAGAGAGGATCCCTGTGACCCCCCC
  TGTAGCCCCTGTGAAGAAAAACCAAGTGCTACTTGCAGTTGGTATAGGAGATCCTGAAGCACCTGGTGTG
  GCACATCTGCAGGACTGCCTAGCTGGTAGTGGCACAGATGTGGAGGACAAGACTGCACTGGATGTCCCTC
  TAGAGAGAAACCACACACCCATGGTGATCAACAGTGATACAGATGAGGAGGAGGAGGAGGAGGAGGAGGA
  GGTCTCAGCAGCACTCACTTTGGCCCATCTGAAAGAGAGAGGAATTGGTTTGTGGAGTAGAGACCCAGGC
  GCAGAAGAGGTCAAGTCCCAACCACAGGTCCTTGTAGAACAAAGCCAGAGTGCCTCTGGGAGAGACAGTG
  ACACGGATGTGGAGGAGGAATCCTCAGGGCGAAAGAGAGAGATTATCCCAGACAGTCCCATGGATGTAGA
  TGAGGCTCTTACTGTCACACAGCCAGAGAGCCAGCCTCCCCGTAGGCCCAATGATGCAGATGAGTACATG
  GATATGAGCTCCCCTGGCAGCCATCTAGTGGTAAATCAGGCCTCCTTTGCTGTGGTAGGCAAGACCAGAG
  CACAAGTGGAGGAGGAAGTCCCAGGGCCATCTGTTATCCTGGGGGAGAAGCACCAGGTGCCTCTAGAGGG
  AGCCCAGCCTCCTGAGGAAGCCTGGGAAACAGCTGTTCAGGAAGGCTCATCCTCACCAGAGGCAGCAGCC
  TCTGTAAGGCCTAGCCAGCAGCCAGTAGCAGAAGATGCTGGGACAGAGTGTGCTACAGCTGTTTCTGAGC
  AGGAAAGTACTCTTGAGGTGAGGTCCCAAAGCGGGTCACCTGCAGCACCAGTGGAGCAAGTGGTGATACA
  TACAGATACTTCAGGGGATCCCACCCTGCCACAGAGAGAGGGAGCCCAAACCCCCACAGGAAGGGAGAGA
  GAAGCACATGTGGGCAGGACCAAGAGTGCCAAAGAGTGCTGTGATGCAGAACCTGAAGATCTGTGCCTTC
  CAGCTACCCAGTGCTTTGTGGAAGGGGAGAGCCAGCACCCAGAAGCTGTCCAGAGTTTGGAAAATGAGCC
  TACCCAACTCTTCCCATGTACTCTTCCACAAGAGCCAGGACCTTCCCATCTCAGCCTTCAGACTCCAGGT
  GCAGATACCTTGGATGTGCCTTGGGAAGTCTTGGCTACACAGCCGTTCTGTCTAAGAGAACAGTCAGAGA
  CTTCTGAGCTTCATGAAGCTCATGGATCTCAACCATCTCTGCCTAGGGAACCACCAGGACACCAACATCT
  GGTTCATACCAGCCCAGTTCACACAGAGCTGTTGAGAATTGAAGGCAGAGAGATACAGACTGTGGAGAAA
  GCCATGGGTATACCAAAGGAAATGGCAGACAGGATGACCCCTGAGAGAGAGCCATTGGAGAGGGAAATCA
  GGGGAAGAACAGAAAACAGTGAGAGAGATGTGATAGGAGAAGAACTAATTCAGGGGACAAAGGACAGAGA
  GCCAAAGAAGGTGTTAGCTAGAGACAGTCAGAGAAAAGAGGCTGACAAGGATCTTGAAGGGAATAGGGAG
  AGTTTGGAGGTAGAAATAGAGATGTCCAAAGACTCACAGAAGAGAGAGAGAAAAGTAGAGAAACCAGAAC
  CAAAGAGAGAATGGGAGCCAGCTGATTTAGAAGTGACTCCAGATAGAGGGGTGACAGAGGAAGGGAGCCA
  TGATCAGAAAGGGCAAATTGCTAGTCTAACACTAAAGCCTGGAGTTGGGGTGAAGGACCTTGAGGGACTT
  GCATCAGCCCCAATAATCACAGGGAGCCAGGCAGATGGAGGAAAGGGAGACCCTTTGAGCCCTGGGAGGC
  AGCAGAGAGGCCGCTTGAGTTGCCAGACGACACCTGCTGGAAAGGCTTCCAGGGGTGATCCAGAGCCCCC
  AGACCACTGCCTGTTTTCTTCAGTGCCTGAAGCTTCAACTCAGAGTCTCCTCACCTCTCAGAGCCAAAAG
  CAGTCTACACCTCAGCCTCTGTTTTCGACCTCTTCTTCTGAGATACCCCTTCCTGAGAGTCTTCACACAA
  AGCCTAATGTCAGGCCACGGCGGTCCTCCAGGATGACCCCCTCCCCGCATTCCTCTGCTGCCCTTAAGCC
  AAATACTACCTGCCCCACAAACCAGCCTGCTGCCTCTAGACCAACATCCCGACCCACTCGGGGCAGGGCA
  AATAGGTCCTCTACCAGGACCCCAGAACTGATTGTCCCTGTAGACCCTGAACTCCAGCCTTCCACCTCCA
  CAGAACAGCCTGTCATCCCAAAACTTACGTCCCAGGTCACTGAGGGCAGGGTACAGATGCCTGAACCACT
  TCTCACAGGTCCCGAGATCCAGTCTCCCACCTCCACAGAACAGTCTGTCACCCCAGACCGCAAACCTCGG
  GCCACTCGGGGCAGGCCAAGTAAATCTCCCAACAAGACCCCAGAACCACTTATTTCTACTGGCCCTGAAC
  TCCAACCTCCCACCTCCATAGAACAACCTGTCATACCTAAACCCACTTCTCGGGTCACTCGGGGCAGACC
  ACGTAAGTCTTCTGTCAGGACCCCAGAATCAGTTGTCTCCACTGGCCCTGAACTTCAGCCTCTCACCTCT
  ATAGAACAGCCTGTTATCCCTGAACCTAGGGCCACTCGAGGTAGACCAAGTAAGTCTTCTATCAAGACCC
  CAGAATCAGTTGTCCCCACTGGTCCTGAGCTGCAGCCTCTTACCTCTGCAAAACAGCCTGTCACCCCTAA
  CCTCACATCTCGGGCCTCTCGGGGTAGATCAAGTAAGTCTATCAGGACCCCGGAACCAGTTGTCCAAACT
  GGCCCTGAATTCCATCCATCCACCTCCACAGAACAGCCTGATACCCGTGAGCCCTCATCTCAGGCTAGGA
  CACGTAGGTCTGCTGTCAAGACTCCTGAAGCGAGTGTCCCCACAACCCCTGAACTCCAGCCTTTCACTTC
  TAAAAAACAGCCTGCCCCTAAGCCTACAGCTCTGGTCACTCAGGGAAGGACATATAAACCCTCCACTGAA
  GATTGTGAATCAGTTGGGCCAGTGGCCCCTGATTTTGAGCCTTCCACCTCTACAGACCATCTTGTCACCC
  CTAAGGTTACAGATCAGAGCTTAACACTACAGTCTTCACCCCTAAGTGCTTCCCCAGTTTCCAGTACCCC
  TGATCTCAAGCCTCCTGTCCCCATAGCCCAGCCTGTTACCCCAGAGCCCATTCCACAAGCCAATCACCAA
  AGGAAACGAAGGGCTGCTGGGAAGCAGGGTTCCCGCACAGTCCCTCTGGGTCATAAGTCTTACTCTGCCC
  TCTCTGAACCTGAGCCCCAATCTTCAGCCAGCCAAAGCTCAGGAGCATCAGAAGCAGACTCACCCCGTCA
  AAAACGTCCCAGAAGACAAGCCTCCCAGAAAACTGTAGTCATCAAGGAAGAACCTGTAGAAACAGAAGTG
  AAGGAAGAGCCTCAAGAGACTGCAATTCCAACACCAGAAAAGAGAAAGAGGGACCATGCAGAAGAAGTGA
  CCCAGGGAAAACCAACTCGAAGCCGGCGAACTAAACCTAACCAAGAAACAGCCCCCAAGGTACTGTTCAC
  AGGAGTCATGGATTCTCGTGGCGAGCGTGCAGTGCTGGCTCTGGGAGGCAGTCTGGCCAGCTCAGTAAAT
  GAGGCCTCCCACTTAGTTACTGATCGCATCCGCAGGACAGTCAAGTTCTTGTGTGCCCTGGGGAAGGGAA
  TTCCCATCCTGTCCCTGAACTGGCTGTACCAGTCCCGAAAGGCTGGTTGCTTCTTGCCGCCTGATGACTA
  CTTAGTGACTGATCCTGAGCAAGAGAAGAACTTTAGCTTCAGCCTTCGGGATTCCCTGTGCCGGGCTCGG
  GAACGAAGACTGCTGGAGGACTATGAGATTCATGTGACCCCTGGCGTGCAGCCACCCCCCCCTCAGATGG
  GCGAGATCATCAGTTGCTGTGGAGGCACTTTCCTGCCCAGCATGCCTCACTCCTATAAGCTTCACCGAGT
  CATCATAACTTGCACTGAAGACCTACCACGCTGTGCTATTCCATCCCGCCTGGGGCTACCCCTCCTCTCT
  CCTGAGTTCCTCCTGACTGGAGTGCTGAAGCAGGAAGCCACACCAGAGGCCTTTGTTCTCTCCAATTTGG
  AAATGTCATCTACCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001010833
• Insert Size: 5127 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001010833.2, NP_001010833.2
• RefSeq Size: 7372 bp
• RefSeq ORF: 5127 bp
• Locus ID: 240087
• Cytogenetics: 17 B1
• Gene Summary: The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 7 divergent copies of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. Mice with mutations in this gene exhibit growth retardation, male infertility, immune defects, chromosome instability, DNA repair defects, and radiation sensitivity. [provided by RefSeq, Jul 2008]