Pnoc (NM_001205075) Mouse Untagged Clone

CAT#: MC226334

Pnoc (untagged) - Mouse prepronociceptin (Pnoc), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pnoc
• Synonyms: N/OFQ; Npnc1; OFQ/N
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226334 representing NM_001205075
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAATCCTCTTTTGTGACGTTCTGCTGCTCAGCCTGCTCTCCAGCGTGTTCAGCAGCTGTCCCAGGG
  ACTGCCTCACCTGCCAGGAGAAGCTCCACCCAGCTCCAGACAGCTTCAACTTAAAGACGTGCATCCTCCA
  GTGTGAAGAGAAGGTCTTCCCCCGCCCTCTCTGGACTGTATGCACCAAAGTCATGGCCAGTGGCTCCGGG
  CAGCTCAGCCCTGCTGACCCAGAGCTTGTGTCAGCTGCTCTTTACCAGCCAAAGGCCTCGGAGATGCAGC
  ACCTGAAGAGAATGCCGCGTGTCCGGAGCTTGGTGCAAGTGCGAGATGCAGAGCCTGGCGCAGATGCTGA
  GCCTGGCGCAGATGCTGAGCCTGGCGCAGATGACGCTGAGGAGGTGGAGCAGAAGCAGCTGCAGAAAAGG
  TTTGGGGGCTTCACCGGGGCCCGGAAATCAGCCCGGAAGTTGGCCAACCAGAAGCGGTTCAGTGAGTTTA
  TGAGGCAGTACCTGGTCCTGAGCATGCAGTCAAGTCAACGCCGGCGCACCCTGCACCAGAATGGCATCCA
  GGTGATCCCCCGTACAGCATGTGTCCACTCCAAGACCTGCAGGCCGGGAGTCAGGATTCCTCCCTCCCCG
  AGGCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001205075
• ORF Size: 639 bp
• Insert Size: 639
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001205075.1, NP_001192004.1
• RefSeq Size: 2101
• RefSeq ORF: 639
• Locus ID: 18155
• Gene Summary: This gene encodes the precursor for neuropeptides that have been implicated in a wide range of physiological roles such as transmission and sensitivity to pain, learning, memory, anxiety and depression, in the central nervous system. The encoded protein is a precursor that is proteolytically processed to generate multiple biologically active peptides including nociceptin and nocistatin which have opposite functions in pain transmission. Mice lacking the encoded protein display increased anxiety, elevated basal pain threshold and impaired adaptation to repeated stress. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]
  Transcript Variant: This variant (2) uses an alternate donor splice site at the penultimate exon compared to variant 1. This results in a frameshift, and a longer isoform (2, also known as N27K) with a distinct C-terminus, compared to isoform 1.