Casp8 (NM_001277926) Mouse Untagged Clone

CAT#: MC228049

Casp8 (untagged) - Mouse caspase 8 (Casp8), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Casp8
• Synonyms: CASP-8; FLICE; MACH; Mch5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228049 representing NM_001277926
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGTGCTTTTGGAACTACTGAGTGACTGGTTACAGCTCTTCTACCTCTTGATAAGAATGGATTTCC
  AGAGTTGTCTTTATGCTATTGCTGAAGAACTGGGCAGTGAAGACCTGGCTGCCCTCAAGTTCCTGTGCTT
  GGACTACATCCCACACAAGAAGCAGGAGACCATCGAGGATGCCCAGAAGCTATTTCTGAGGCTGCGGGAA
  AAGGGGATGTTGGAGGAAGGCAATCTGTCTTTCCTGAAAGAGCTGCTTTTCCACATCAGTCGGTGGGACC
  TGCTGGTCAACTTCCTAGACTGCAACCGAGAGGAGATGGTGAGAGAGCTGCGGGATCCAGACAATGCCCA
  GATTTCTCCCTACAGGGTCATGCTCTTTAAGCTCTCAGAAGAAGTGAGCGAGTTGGAATTGAGATCTTTT
  AAGTTCCTTTTGAACAATGAGATCCCCAAATGTAAGCTGGAAGATGACTTGAGCCTGCTTGAAATTTTTG
  TAGAAATGGAGAAGAGGACCATGCTGGCAGAAAATAACTTGGAAACCCTAAAATCAATCTGTGACCAGGT
  CAACAAGAGCCTGCTGGGGAAGATCGAGGATTATGAAAGATCAAGCACAGAGAGAAGAATGAGCCTTGAA
  GGAAGGGAAGAGTTGCCACCTTCAGTTTTGGATGAGATGAGCCTCAAAATGGCGGAACTGTGTGACTCGC
  CAAGAGAACAAGACAGTGAGTCACGGACTTCAGACAAAGTTTACCAAATGAAGAACAAACCTCGGGGATA
  CTGTCTGATCATCAACAATCATGATTTCAGCAAGGCCCGGGAAGACATAACCCAACTCCGAAAAATGAAG
  GACAGAAAAGGAACAGACTGTGATAAAGAGGCTCTGAGTAAGACCTTTAAGGAGCTTCATTTTGAGATAG
  TATCTTACGACGACTGCACTGCAAATGAAATCCACGAGATTCTAGAAGGCTACCAAAGCGCAGACCACAA
  GAACAAAGACTGCTTCATCTGCTGTATCCTATCCCACGGTGACAAGGGTGTCGTCTATGGAACGGATGGG
  AAGGAGGCCTCCATCTATGACCTGACATCTTACTTCACTGGTTCAAAGTGCCCTTCCCTGTCTGGGAAAC
  CCAAGATCTTTTTCATTCAGGCTTGCCAAGGAAGTAACTTCCAGAAAGGAGTGCCTGATGAGGCAGGCTT
  CGAGCAACAGAACCACACTTTAGAAGTGGATTCATCATCTCACAAGAACTATATTCCGGATGAGGCAGAC
  TTTCTGCTGGGAATGGCTACGGTGAAGAACTGCGTTTCCTACCGAGATCCTGTGAATGGAACCTGGTATA
  TTCAGTCACTTTGCCAGAGCCTGAGGGAAAGATGTCCTCAAGGAGATGACATTCTTAGCATCCTGACTGG
  CGTGAACTATGACGTGAGCAATAAAGACGACAGGAGGAACAAGGGAAAGCAGATGCCACAGCCCACCTTC
  ACACTACGGAAGAAGCTCTTCTTCCCTCCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001277926
• ORF Size: 1503 bp
• Insert Size: 1503
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001277926.1, NP_001264855.1
• RefSeq Size: 2528
• RefSeq ORF: 1503
• Locus ID: 12370
• Gene Summary: This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, an initiator of apoptotic cell death, is activated by death-inducing tumor necrosis family receptors and targets downstream effectors. In mouse deficiency of this gene can cause embryonic lethality. This protein may have a role in embryogenesis. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Apr 2013]
  Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region and uses an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct and longer N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.