Casp8 (NM_001277926) Mouse Tagged ORF Clone

CAT#: MR230260

Casp8 (myc-DDK-tagged) - Mouse caspase 8 (Casp8), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Casp8
• Synonyms: CASP-8; FLICE; MACH; Mch5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230260 representing NM_001277926
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGTGCTTTTGGAACTACTGAGTGACTGGTTACAGCTCTTCTACCTCTTGATAAGAATGGATTTCC
  AGAGTTGTCTTTATGCTATTGCTGAAGAACTGGGCAGTGAAGACCTGGCTGCCCTCAAGTTCCTGTGCTT
  GGACTACATCCCACACAAGAAGCAGGAGACCATCGAGGATGCCCAGAAGCTATTTCTGAGGCTGCGGGAA
  AAGGGGATGTTGGAGGAAGGCAATCTGTCTTTCCTGAAAGAGCTGCTTTTCCACATCAGTCGGTGGGACC
  TGCTGGTCAACTTCCTAGACTGCAACCGAGAGGAGATGGTGAGAGAGCTGCGGGATCCAGACAATGCCCA
  GATTTCTCCCTACAGGGTCATGCTCTTTAAGCTCTCAGAAGAAGTGAGCGAGTTGGAATTGAGATCTTTT
  AAGTTCCTTTTGAACAATGAGATCCCCAAATGTAAGCTGGAAGATGACTTGAGCCTGCTTGAAATTTTTG
  TAGAAATGGAGAAGAGGACCATGCTGGCAGAAAATAACTTGGAAACCCTAAAATCAATCTGTGACCAGGT
  CAACAAGAGCCTGCTGGGGAAGATCGAGGATTATGAAAGATCAAGCACAGAGAGAAGAATGAGCCTTGAA
  GGAAGGGAAGAGTTGCCACCTTCAGTTTTGGATGAGATGAGCCTCAAAATGGCGGAACTGTGTGACTCGC
  CAAGAGAACAAGACAGTGAGTCACGGACTTCAGACAAAGTTTACCAAATGAAGAACAAACCTCGGGGATA
  CTGTCTGATCATCAACAATCATGATTTCAGCAAGGCCCGGGAAGACATAACCCAACTCCGAAAAATGAAG
  GACAGAAAAGGAACAGACTGTGATAAAGAGGCTCTGAGTAAGACCTTTAAGGAGCTTCATTTTGAGATAG
  TATCTTACGACGACTGCACTGCAAATGAAATCCACGAGATTCTAGAAGGCTACCAAAGCGCAGACCACAA
  GAACAAAGACTGCTTCATCTGCTGTATCCTATCCCACGGTGACAAGGGTGTCGTCTATGGAACGGATGGG
  AAGGAGGCCTCCATCTATGACCTGACATCTTACTTCACTGGTTCAAAGTGCCCTTCCCTGTCTGGGAAAC
  CCAAGATCTTTTTCATTCAGGCTTGCCAAGGAAGTAACTTCCAGAAAGGAGTGCCTGATGAGGCAGGCTT
  CGAGCAACAGAACCACACTTTAGAAGTGGATTCATCATCTCACAAGAACTATATTCCGGATGAGGCAGAC
  TTTCTGCTGGGAATGGCTACGGTGAAGAACTGCGTTTCCTACCGAGATCCTGTGAATGGAACCTGGTATA
  TTCAGTCACTTTGCCAGAGCCTGAGGGAAAGATGTCCTCAAGGAGATGACATTCTTAGCATCCTGACTGG
  CGTGAACTATGACGTGAGCAATAAAGACGACAGGAGGAACAAGGGAAAGCAGATGCCACAGCCCACCTTC
  ACACTACGGAAGAAGCTCTTCTTCCCTCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230260 representing NM_001277926
  Red=Cloning site Green=Tags(s)
  
  MCVLLELLSDWLQLFYLLIRMDFQSCLYAIAEELGSEDLAALKFLCLDYIPHKKQETIEDAQKLFLRLRE
  KGMLEEGNLSFLKELLFHISRWDLLVNFLDCNREEMVRELRDPDNAQISPYRVMLFKLSEEVSELELRSF
  KFLLNNEIPKCKLEDDLSLLEIFVEMEKRTMLAENNLETLKSICDQVNKSLLGKIEDYERSSTERRMSLE
  GREELPPSVLDEMSLKMAELCDSPREQDSESRTSDKVYQMKNKPRGYCLIINNHDFSKAREDITQLRKMK
  DRKGTDCDKEALSKTFKELHFEIVSYDDCTANEIHEILEGYQSADHKNKDCFICCILSHGDKGVVYGTDG
  KEASIYDLTSYFTGSKCPSLSGKPKIFFIQACQGSNFQKGVPDEAGFEQQNHTLEVDSSSHKNYIPDEAD
  FLLGMATVKNCVSYRDPVNGTWYIQSLCQSLRERCPQGDDILSILTGVNYDVSNKDDRRNKGKQMPQPTF
  TLRKKLFFPP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001277926
• ORF Size: 1500 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001277926.1, NP_001264855.1
• RefSeq Size: 2528
• RefSeq ORF: 1503
• Locus ID: 12370
• MW: 58.3 kDa
• Gene Summary: This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, an initiator of apoptotic cell death, is activated by death-inducing tumor necrosis family receptors and targets downstream effectors. In mouse deficiency of this gene can cause embryonic lethality. This protein may have a role in embryogenesis. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Apr 2013]