Crem (BC034856) Mouse Tagged ORF Clone

CAT#: MG200636

Crem (GFP-tagged) - Mouse cAMP responsive element modulator (cDNA clone MGC:41125 IMAGE:3373396)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Crem
• Synonyms: ICER|ICERI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200636 representing BC034856
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCATGGAAACAGTTGAATCACAGCAGGATCGAAGTGTAACACGTTCTGTGGCAGAGCATAGCTCTG
  CTCATATGCAGACTGGTCAAATTTCTGTTCCTACTCTAGCTCAGGTAGCAACAATTGCAGAGACAGATGA
  TTCTGCAGACTCAGAAGTAATTGATTCGCATAAACGTAGAGAAATTCTTTCACGAAGACCCTCATATAGA
  AAAATACTGAATGAACTTTCCTCTGATGTGCCTGGTATTCCCAAGATTGAAGAAGAAAAATCAGAGGAAG
  AAGGGACACCACCTAACATTGCTACCATGGCAGTACCAACTAGCATATATCAGACTAGCACGGGGCAATA
  CAGTATGTATGCTATGATTCCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200636 representing BC034856
  Red=Cloning site Green=Tags(s)
  
  MTMETVESQQDRSVTRSVAEHSSAHMQTGQISVPTLAQVATIAETDDSADSEVIDSHKRREILSRRPSYR
  KILNELSSDVPGIPKIEEEKSEEEGTPPNIATMAVPTSIYQTSTGQYSMYAMIP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC034856
• ORF Size: 374 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC034856, AAH34856
• RefSeq Size: 941
• RefSeq ORF: 374
• Locus ID: 12916
• Gene Summary: This gene encodes a basic-leucine zipper domain-containing protein that localizes to gene promoters, where it binds to the cyclic AMP response element (CRE). Different protein isoforms encoded by this gene may function as either activators or repressors of transcription. Activity of this gene is important in multiple developmental processes, including spermatogenesis. Mutation of this gene causes male infertility. Alternative splicing and promoter usage result in multiple transcript variants for this gene. [provided by RefSeq, Oct 2012]