Mafk (NM_010757) Mouse Tagged ORF Clone

CAT#: MG201177

Mafk (GFP-tagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein K (avian) (Mafk)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mafk
• Synonyms: AW061068; NF-E2; Nfe2u
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201177 representing NM_010757
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGACTAATCCCAAGCCCAACAAGGCATTGAAGGTCAAGAAGGAGGCGGGTGAGAACGCCCCTGTTC
  TTAGCGATGATGAGCTGGTGTCCATGTCAGTGCGGGAGCTAAACCAGCACCTGCGGGGGCTCACCAAGGA
  GGAGGTCACTCGGCTGAAGCAGCGGCGGCGCACACTCAAGAACAGAGGCTACGCGGCTAGCTGTCGCATC
  AAGCGTGTGACACAGAAAGAGGAGCTGGAACGGCAGCGTGTGGAGCTACAGCAGGAGGTGGAGAAGCTGG
  CTCGAGAGAACAGCAGCATGCGGCTGGAGCTAGATGCCCTGCGCTCCAAGTATGAGGCCCTACAGACCTT
  CGCTCGCACCGTGGCCCGAGGGCCTGTCACACCCACCAAGGTGGCCACCACCAGTGTCATCACCATCGTC
  AAATCTGCCGAGCTCTCCTCCACCTCTGTACCCTTCTCAGCCGCCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201177 representing NM_010757
  Red=Cloning site Green=Tags(s)
  
  MTTNPKPNKALKVKKEAGENAPVLSDDELVSMSVRELNQHLRGLTKEEVTRLKQRRRTLKNRGYAASCRI
  KRVTQKEELERQRVELQQEVEKLARENSSMRLELDALRSKYEALQTFARTVARGPVTPTKVATTSVITIV
  KSAELSSTSVPFSAAS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010757
• ORF Size: 468 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010757.2, NP_034887.1
• RefSeq Size: 2825
• RefSeq ORF: 471
• Locus ID: 17135
• Gene Summary: Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. [UniProtKB/Swiss-Prot Function]