Psmb6 (NM_008946) Mouse Tagged ORF Clone

CAT#: MG202087

Psmb6 (GFP-tagged) - Mouse proteasome (prosome, macropain) subunit, beta type 6 (Psmb6)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Psmb6
• Synonyms: Lmp19; Mpnd
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202087 representing NM_008946
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTGCAGTTTAATGGGGGCGTGGTTCTAGGAGCGGACTCCAGGACAACCACTGGGTCCTACATCG
  CCAATCGAGTGACTGACAAGCTGACCCCTATCCACGATCACATCTTCTGCTGCCGCTCAGGCTCAGCCGC
  TGATACCCAAGCAGTGGCAGACGCTGTCACTTACCAGCTTGGTTTCCACAGTATTGAACTGAACGAGCCT
  CCACTAGTCCACACAGCCGCCAGTCTCTTTAAGGAGATGTGTTACCGGTACAGAGAAGATCTGATGGCAG
  GAATCATCATTGCAGGCTGGGACCCTCAAGAAGGAGGGCAGGTGTACTCTGTTCCCATGGGGGGTATGAT
  GGTAAGACAGTCCTTTGCCATCGGAGGCTCCCGGAGCTCGTACATCTATGGCTATGTTGATGCTACGTAT
  CGGGAAGGCATGACCAAGGACGAATGTCTGCAGTTCACTGCCAATGCTCTCGCTTTGGCCATGGAACGCG
  ACGGCTCCAGTGGAGGGGTGATCCGCTTGGCAGCCATTCAGGAGTCAGGGGTAGAGCGGCAGGTGCTTTT
  GGGAGACCAAATCCCCAAGTTCACCATTGCCACGTTGCCACCTCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202087 representing NM_008946
  Red=Cloning site Green=Tags(s)
  
  MAVQFNGGVVLGADSRTTTGSYIANRVTDKLTPIHDHIFCCRSGSAADTQAVADAVTYQLGFHSIELNEP
  PLVHTAASLFKEMCYRYREDLMAGIIIAGWDPQEGGQVYSVPMGGMMVRQSFAIGGSRSSYIYGYVDATY
  REGMTKDECLQFTANALALAMERDGSSGGVIRLAAIQESGVERQVLLGDQIPKFTIATLPPP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008946
• ORF Size: 606 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008946.2, NP_032972.2
• RefSeq Size: 805
• RefSeq ORF: 717
• Locus ID: 19175
• Gene Summary: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. May catalyze basal processing of intracellular antigens. [UniProtKB/Swiss-Prot Function]