Eif4e (BC010759) Mouse Tagged ORF Clone

CAT#: MG202403

Eif4e (GFP-tagged) - Mouse eukaryotic translation initiation factor 4E (cDNA clone MGC:18497 IMAGE:4216609)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Eif4e
• Synonyms: Eif4e-ps, eIF-4E
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202403 representing BC010759
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACTGTGGAACCGGAAACCACCCCTACCACTAATCCCCCACCTGCAGAAGAGGAAAAAACAGAGT
  CTAATCAGGAGGTTGCTAACCCAGAGCACTATATTAAACACCCTCTACAGAACAGGTGGGCACTCTGGTT
  TTTTAAAAATGATAAAAGCAAAACTTGGCAAGCAAACCTTCGATTGATCTCTAAGTTTGATACTGTTGAA
  GACTTTTGGGCTCTATACAACCATATCCAGTTGTCTAGTAATTTAATGCCTGGCTGTGACTACTCACTTT
  TTAAGGACGGGATTGAGCCTATGTGGGAAGATGAGAAAAACAAACGAGGAGGACGGTGGCTGATCACACT
  GAACAAGCAGCAGAGACGGAGTGACCTCGATCGCTTCTGGCTAGAGACACTGCTGTGCCTTATTGGAGAA
  TCTTTCGATGACTACAGTGATGATGTGTGTGGAGCTGTTGTTAATGTTAGAGCTAAAGGTGATAAGATAG
  CAATATGGACTACTGAGTGTGAAAACAGAGATGCAGTCACACACATAGGGAGGGTATACAAGGAAAGGTT
  AGGACTTCCTCCGAAGATAGTGATTGGTTATCAGTCCCACGCAGACACAGCTACAAAGAGCGGCTCCACC
  ACTAAAAATAGGTTTGTTGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202403 representing BC010759
  Red=Cloning site Green=Tags(s)
  
  MATVEPETTPTTNPPPAEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVE
  DFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGE
  SFDDYSDDVCGAVVNVRAKGDKIAIWTTECENRDAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGST
  TKNRFVV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC010759
• ORF Size: 651 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC010759.1
• RefSeq Size: 1646
• RefSeq ORF: 653
• Locus ID: 13684
• Gene Summary: This gene encodes a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. It has also been associated with autism spectrum disorders. Consistently, knockout of this gene results in increased translation of neuroligins, postsynaptic proteins linked to autism spectrum disorders. Pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]