B3galt5 (NM_033149) Mouse Tagged ORF Clone

CAT#: MG204357

B3galt5 (GFP-tagged) - Mouse UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 (B3galt5)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: B3galt5
• Synonyms: 1190002B21Rik; AU045265; b3Galt-V
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204357 representing NM_033149
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCACATGAAGACAAGGCTAGTTTACGCCTCCATTCTCATGATGGGCGCACTCTGCTTGTACTTCA
  GCATGGATTCTTTCAGAGAACTCCCGTTTGTTTTTAAGAAAAGTCACGGGAAGTTCCTTCAGATTCCGGA
  TATAGACTGCAAGCAGAAGCCGCCTTTCCTGGTGCTGCTGGTGACGTCATCTCACAAGCAGCTGGCCGCT
  CGCATGGCCATCCGCAAGACGTGGGGTAGAGAGACATCTGTGCAGGGCCAACAGGTGAGGACCTTCTTCC
  TTCTGGGGACCTCCGACAGCACCGAGGAGATGGACGCCACAACCCTGGAGAGCGAGCAGCACCGCGACAT
  TATCCAGAAGGACTTCAAGGATGCCTACTTCAACCTGACCCTGAAGACCATGATGGGTATGGAATGGGTC
  TACCACTTTTGTCCTCAGACAGCTTACGTGATGAAAACGGACTCTGACATGTTTGTGAATGTTGGCTATC
  TGACGGAACTGCTGCTAAAGAAAAACAAAACGACCAGGTTCTTCACAGGCTACATAAAGCCCCACGACTT
  TCCCATCCGGCAGAAGTTCAACAAGTGGTTTGTGAGTAAGTTCGAATATCCCTGGGACAGGTACCCACCT
  TTTTGCTCCGGTACTGGTTATGTCTTTTCCAGCGACGTGGCGATCCAAGTATACAACGTCTCAGAGAGCG
  TTCCGTTCATCAAGCTGGAGGATGTGTTTGTTGGGCTCTGCTTGGCCAAGCTAAAGATCCGGCCGGAGGA
  GCTGCACACCAAACAGACCTTCTTCCCTGGCGGTTTACGCTTTTCCGTGTGCCGCTTTCAGAAAATTGTG
  GCATGCCATTTTATGAAGCCCCAGGACCTGCTCACTTACTGGCAAGCACTGGAGAACTCGAAAGAACAGG
  ACTGTCCTGCTGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204357 representing NM_033149
  Red=Cloning site Green=Tags(s)
  
  MAHMKTRLVYASILMMGALCLYFSMDSFRELPFVFKKSHGKFLQIPDIDCKQKPPFLVLLVTSSHKQLAA
  RMAIRKTWGRETSVQGQQVRTFFLLGTSDSTEEMDATTLESEQHRDIIQKDFKDAYFNLTLKTMMGMEWV
  YHFCPQTAYVMKTDSDMFVNVGYLTELLLKKNKTTRFFTGYIKPHDFPIRQKFNKWFVSKFEYPWDRYPP
  FCSGTGYVFSSDVAIQVYNVSESVPFIKLEDVFVGLCLAKLKIRPEELHTKQTFFPGGLRFSVCRFQKIV
  ACHFMKPQDLLTYWQALENSKEQDCPAV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033149
• ORF Size: 924 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_033149.2, NP_149161.1
• RefSeq Size: 4933
• RefSeq ORF: 927
• Locus ID: 93961
• Gene Summary: Catalyzes the transfer of Gal to GlcNAc-based acceptors with a preference for the core3 O-linked glycan GlcNAc(beta1,3)GalNAc structure. Can use glycolipid LC3Cer as an efficient acceptor. Also catalyzes the transfer of Gal to the terminal GalNAc unit of the globoside GB4, thereby synthesizing the glycolipid GB5, also known as the stage-specific embryonic antigen-3 (SSEA-3). [UniProtKB/Swiss-Prot Function]