B3galt5 (NM_033149) Mouse Tagged ORF Clone

CAT#: MR204357

B3galt5 (Myc-DDK-tagged) - Mouse UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 (B3galt5), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: B3galt5
• Synonyms: 1190002B21Rik; AU045265; b3Galt-V
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204357 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCACATGAAGACAAGGCTAGTTTACGCCTCCATTCTCATGATGGGCGCACTCTGCTTGTACTTCA
  GCATGGATTCTTTCAGAGAACTCCCGTTTGTTTTTAAGAAAAGTCACGGGAAGTTCCTTCAGATTCCGGA
  TATAGACTGCAAGCAGAAGCCGCCTTTCCTGGTGCTGCTGGTGACGTCATCTCACAAGCAGCTGGCCGCT
  CGCATGGCCATCCGCAAGACGTGGGGTAGAGAGACATCTGTGCAGGGCCAACAGGTGAGGACCTTCTTCC
  TTCTGGGGACCTCCGACAGCACCGAGGAGATGGACGCCACAACCCTGGAGAGCGAGCAGCACCGCGACAT
  TATCCAGAAGGACTTCAAGGATGCCTACTTCAACCTGACCCTGAAGACCATGATGGGTATGGAATGGGTC
  TACCACTTTTGTCCTCAGACAGCTTACGTGATGAAAACGGACTCTGACATGTTTGTGAATGTTGGCTATC
  TGACGGAACTGCTGCTAAAGAAAAACAAAACGACCAGGTTCTTCACAGGCTACATAAAGCCCCACGACTT
  TCCCATCCGGCAGAAGTTCAACAAGTGGTTTGTGAGTAAGTTCGAATATCCCTGGGACAGGTACCCACCT
  TTTTGCTCCGGTACTGGTTATGTCTTTTCCAGCGACGTGGCGATCCAAGTATACAACGTCTCAGAGAGCG
  TTCCGTTCATCAAGCTGGAGGATGTGTTTGTTGGGCTCTGCTTGGCCAAGCTAAAGATCCGGCCGGAGGA
  GCTGCACACCAAACAGACCTTCTTCCCTGGCGGTTTACGCTTTTCCGTGTGCCGCTTTCAGAAAATTGTG
  GCATGCCATTTTATGAAGCCCCAGGACCTGCTCACTTACTGGCAAGCACTGGAGAACTCGAAAGAACAGG
  ACTGTCCTGCTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204357 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAHMKTRLVYASILMMGALCLYFSMDSFRELPFVFKKSHGKFLQIPDIDCKQKPPFLVLLVTSSHKQLAA
  RMAIRKTWGRETSVQGQQVRTFFLLGTSDSTEEMDATTLESEQHRDIIQKDFKDAYFNLTLKTMMGMEWV
  YHFCPQTAYVMKTDSDMFVNVGYLTELLLKKNKTTRFFTGYIKPHDFPIRQKFNKWFVSKFEYPWDRYPP
  FCSGTGYVFSSDVAIQVYNVSESVPFIKLEDVFVGLCLAKLKIRPEELHTKQTFFPGGLRFSVCRFQKIV
  ACHFMKPQDLLTYWQALENSKEQDCPAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033149
• ORF Size: 927 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033149.1, NM_033149.2, NM_033149.3, NP_149161.1
• RefSeq Size: 4914 bp
• RefSeq ORF: 927 bp
• Locus ID: 93961
• MW: 36 kDa
• Gene Summary: Catalyzes the transfer of Gal to GlcNAc-based acceptors with a preference for the core3 O-linked glycan GlcNAc(beta1,3)GalNAc structure. Can use glycolipid LC3Cer as an efficient acceptor. Also catalyzes the transfer of Gal to the terminal GalNAc unit of the globoside GB4, thereby synthesizing the glycolipid GB5, also known as the stage-specific embryonic antigen-3 (SSEA-3). [UniProtKB/Swiss-Prot Function]