Rad18 (BC011120) Mouse Tagged ORF Clone

CAT#: MG204378

Rad18 (GFP-tagged) - Mouse RAD18 homolog (S. cerevisiae) (cDNA clone MGC:19000 IMAGE:4014013)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rad18
• Synonyms: Rad18sc
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204378 representing BC011120
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTCCTGGCCGAGCCGCGATGGCCTCCGGGGTTGGCGGTAATGAAGACAATAGATGACTTGCTGC
  GCTGTGGGATTTGTTTTGAGTATTTCAACATTGCAGTGATAATCCCCCAGTGCTCTCACAACTATTGTTC
  ACTCTGTATAAGAAAGTTTTTATCCTATAAAACTCAGTGCCCAACTTGTTGCGTGGCAGTAACGGAGCCA
  GACCTGAGAAATAATCGCCTCTTAGATGAACTGGTAAAAAGCATGAATTTTGCACGGACTCACCTGTTGC
  AGTTTGCTTTAGAGTCACCACCCATATCTCCTGTGTCTTCCACCTCAAAGAAGGTTGTTGTTAAAGTGCA
  TAATGCTGACGCCGCCCAACACCCTGTCAAACAGGGGAACAGGTTAATGGATAAGTTCCTGATTAGAGAA
  ACTGGTGACTGTGTATTTGAGTTGTTGGGAAAAGAAAATGAGAGGAAATTCAGCCCTCAAAAAGAGCTAA
  GCACCTCTGCTGAGATTAAAGAGACAAGTCTCCTAGGAAAGCCGGTACTGGGTCTCTCAGATGCTAATGG
  TCCTGTGACTCCCTCTACATCCACTATGAAACTGGATACTAAAGTGTCTTGTCCTGTTTGTGGGGTCAGC
  ATTCCAGAAAATCATATCAATAAGCATTTAGACAGTTGTCTATCACGTGAAGAGAAGAAGGAGAGCCTGC
  GAAGTTCTGCTCACAAAAGGAAGCCGTTGCCCAAAACTGTATATAACTTGCTCTCTGATCGTGATTTAAA
  GAAAAAGCTGAAACAGTATGGCTTATCTGTTCAAGGAAACAAACAGCAGCTTATTAAAAGGCATCAGGAA
  TTTGTGCACATGTATAATGCCCAGTGCGATGCTTTGCATCCTAAATCAGGTCATGGTTTTTACAAAGAAC
  CAAACAGAGAAGGAAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204378 representing BC011120
  Red=Cloning site Green=Tags(s)
  
  MEVLAEPRWPPGLAVMKTIDDLLRCGICFEYFNIAVIIPQCSHNYCSLCIRKFLSYKTQCPTCCVAVTEP
  DLRNNRLLDELVKSMNFARTHLLQFALESPPISPVSSTSKKVVVKVHNADAAQHPVKQGNRLMDKFLIRE
  TGDCVFELLGKENERKFSPQKELSTSAEIKETSLLGKPVLGLSDANGPVTPSTSTMKLDTKVSCPVCGVS
  IPENHINKHLDSCLSREEKKESLRSSAHKRKPLPKTVYNLLSDRDLKKKLKQYGLSVQGNKQQLIKRHQE
  FVHMYNAQCDALHPKSGHGFYKEPNREGN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC011120
• ORF Size: 927 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC011120.1
• RefSeq Size: 1482
• RefSeq ORF: 929
• Locus ID: 58186
• Gene Summary: E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. [UniProtKB/Swiss-Prot Function]