Rad18 (BC011120) Mouse Tagged ORF Clone

CAT#: MR204378

  • TrueORF®

Rad18 (Myc-DDK-tagged) - Mouse RAD18 homolog (S. cerevisiae) (cDNA clone MGC:19000 IMAGE:4014013)


  "BC011120" in other vectors (4)


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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Rad18
Synonyms Rad18sc
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR204378 representing BC011120
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTCCTGGCCGAGCCGCGATGGCCTCCGGGGTTGGCGGTAATGAAGACAATAGATGACTTGCTGC
GCTGTGGGATTTGTTTTGAGTATTTCAACATTGCAGTGATAATCCCCCAGTGCTCTCACAACTATTGTTC
ACTCTGTATAAGAAAGTTTTTATCCTATAAAACTCAGTGCCCAACTTGTTGCGTGGCAGTAACGGAGCCA
GACCTGAGAAATAATCGCCTCTTAGATGAACTGGTAAAAAGCATGAATTTTGCACGGACTCACCTGTTGC
AGTTTGCTTTAGAGTCACCACCCATATCTCCTGTGTCTTCCACCTCAAAGAAGGTTGTTGTTAAAGTGCA
TAATGCTGACGCCGCCCAACACCCTGTCAAACAGGGGAACAGGTTAATGGATAAGTTCCTGATTAGAGAA
ACTGGTGACTGTGTATTTGAGTTGTTGGGAAAAGAAAATGAGAGGAAATTCAGCCCTCAAAAAGAGCTAA
GCACCTCTGCTGAGATTAAAGAGACAAGTCTCCTAGGAAAGCCGGTACTGGGTCTCTCAGATGCTAATGG
TCCTGTGACTCCCTCTACATCCACTATGAAACTGGATACTAAAGTGTCTTGTCCTGTTTGTGGGGTCAGC
ATTCCAGAAAATCATATCAATAAGCATTTAGACAGTTGTCTATCACGTGAAGAGAAGAAGGAGAGCCTGC
GAAGTTCTGCTCACAAAAGGAAGCCGTTGCCCAAAACTGTATATAACTTGCTCTCTGATCGTGATTTAAA
GAAAAAGCTGAAACAGTATGGCTTATCTGTTCAAGGAAACAAACAGCAGCTTATTAAAAGGCATCAGGAA
TTTGTGCACATGTATAATGCCCAGTGCGATGCTTTGCATCCTAAATCAGGTCATGGTTTTTACAAAGAAC
CAAACAGAGAAGGAAAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR204378 representing BC011120
Red=Cloning site Green=Tags(s)

MEVLAEPRWPPGLAVMKTIDDLLRCGICFEYFNIAVIIPQCSHNYCSLCIRKFLSYKTQCPTCCVAVTEP
DLRNNRLLDELVKSMNFARTHLLQFALESPPISPVSSTSKKVVVKVHNADAAQHPVKQGNRLMDKFLIRE
TGDCVFELLGKENERKFSPQKELSTSAEIKETSLLGKPVLGLSDANGPVTPSTSTMKLDTKVSCPVCGVS
IPENHINKHLDSCLSREEKKESLRSSAHKRKPLPKTVYNLLSDRDLKKKLKQYGLSVQGNKQQLIKRHQE
FVHMYNAQCDALHPKSGHGFYKEPNREGN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC011120
ORF Size 927 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq BC011120.1
RefSeq Size 1482
RefSeq ORF 929
Locus ID 58186
MW 54.3 kDa
Gene Summary E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. [UniProtKB/Swiss-Prot Function]

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