Mkrn1 (BC003329) Mouse Tagged ORF Clone

CAT#: MG204844

Mkrn1 (GFP-tagged) - Mouse makorin, ring finger protein, 1 (cDNA clone MGC:7132 IMAGE:3158135)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mkrn1
• Synonyms: RFP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204844 representing BC003329
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGCTGCGGCTCCCGGAACAACAGCCACAACATCAGGAGCAGGAGCGGCAGCGGCGGCGGTGG
  CAGCGGCCTCCCTCACCTCTATCCCCACAGTCGCTGCCCCGTCCCCGGGGGCGGGAGGAGGGGGCGGCGG
  CAGCGACGGCAGCGGCGGCGGCTGGACGAAACAGGTCACCTGCAGATACTTTATGCATGGGGTTTGTAAG
  GAAGGCGATAACTGTCGATACTCTCATGACCTCTCTGACAGTCCATACGGCGTAGTGTGCAAGTATTTTC
  AAAGAGGGTACTGTGTTTATGGAGACCGCTGCAGATACGAACACAGCAAGCCACTGAAACAGGAAGAAGT
  GACTGCTACTGATCTGAGTGCAAAACCATCCCTCGCTGCTTCCTCAAGTCTCTCTTCTGGTGTTGGATCA
  CTTGCTGAAATGAATTCAGGCGAAGCTGAGTCACGAAACCCAAGCTTTCCAACTGTAGGAGCAGGTTCAG
  AGGACTGGGTGAATGCCATTGAGTTTGTCCCTGGGCAGCCCTACTGTGGCCGTACTGCCCCTTCCTGCAC
  TGAAGTACCCCCGCAGGGTTCAGTGACCAAGGAAGAGTCAGAGAAGGAGCCAACTACGGTGGAGACAAAG
  AAGCAGCTTTGCCCCTATGCTGCAGTGGGAGAGTGTCGCTATGGGGAAAACTGTGTGTATCTCCACGGAG
  ACTCGTGTGACATGTGTGGCCTGCAGGTCCTACACCCAGTGGATGCTGCCCAGAGGTCACAGCACATAAA
  ATCTTGCATTGAGGCCCATGAGAAGGACATGGAGCTCTCTTTTGCTGTTCAGCGCAGCAAGGACATGGTG
  TGTGGGATCTGCATGGAGGTGGTCTATGAGAAAGCCAACCCCAGCGAGCGTCGCTTTGGGATCCTCTCCA
  ACTGCAACCACACCTACTGTCTCAAGTGTATTCGCAAGTGGAGAAGTGCTAAACAATTTGAGAGCAAGAT
  CATAAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204844 representing BC003329
  Red=Cloning site Green=Tags(s)
  
  MAEAAAPGTTATTSGAGAAAAAVAAASLTSIPTVAAPSPGAGGGGGGSDGSGGGWTKQVTCRYFMHGVCK
  EGDNCRYSHDLSDSPYGVVCKYFQRGYCVYGDRCRYEHSKPLKQEEVTATDLSAKPSLAASSSLSSGVGS
  LAEMNSGEAESRNPSFPTVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEVPPQGSVTKEESEKEPTTVETK
  KQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPVDAAQRSQHIKSCIEAHEKDMELSFAVQRSKDMV
  CGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC003329
• ORF Size: 989 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC003329, AAH03329
• RefSeq Size: 1523
• RefSeq ORF: 989
• Locus ID: 54484