Pola2 (BC006945) Mouse Tagged ORF Clone

CAT#: MG205518

Pola2 (GFP-tagged) - Mouse polymerase (DNA directed), alpha 2 (cDNA clone MGC:11533 IMAGE:3602559)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pola2
• Synonyms: AI573378
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205518 representing BC006945
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTGTCCACCCAGCAGCTGGCGGAGGAACTACAGATCTTCGGCCTGGACTACGAGGACTCCCTGT
  TGGAGAAACTGGCAGAGCTGTGTGTTCTATACCGACAGACTGAGGACGGGATGGTCAGTGAGCTCATCGC
  CTTCTGCACCAGTGCGGGGAAGACATGCCTTACTGTAGACATCCTGAACTCTTTTGAATACGAGGTTCTG
  AACAAAAAACTCTCCAAAGCCTGGCACAGTGCCTCCAAAGACAGTGGGCATGCAGGAACCAGAGACATTG
  TTTCTATACAAGAGCTAATTGAAGCAGAGGAGGAAGAGGAGACCCTCCTAAGCTCTTATACCACACCCTC
  TAAGGGTCCTCTCAAGCGAGTTAGCAGCACCCCAGAAACCCCCCTAACCAAAAGGAGCGTCGCTGCTCGC
  AGCCCACGTCAGCTTCTCTCACCGTCAAGTTTCTCCCCGAGTGCTACCCCCTCCCAGAAATACACCTCCA
  GAACCAACCGGGGAGAAGTGGTGACCACCTTTGGCTCCGCACAGGGCTTGTCTTGGTCCGGGAGAGGAGG
  CTCTGGAAGCGTCAGTCTAAAGGTCGTGGGGGACCCAGAGCCACTCACTGGCAGCTACAAAGCGATGTTT
  CAGCAGCTCATGGGTGTTCGAGAAGTTCTGACCAGTAAGATCGAAGAGCTCGGCAGCGAGCTGAAGGAGC
  ATCACAAGATCGAGGCCTTTACCCCTCTCCTGGTCCCAGCGCAAGAGCCTGTTATTCTCCTGGGTCAGAT
  TGGCTGCGACAGCAACGGGAAGCTGAACAGCAAGTCCGTGATTCTCGAGGGGGACCAGGAGCACTCCTAT
  GGCGCGCAGATCCCAGTGGACCTATCGGAACTCAAAGAGTATTCCCTCTTCCCTGGACAGGTTGTAATTA
  TGGAAGGATTCAACACCACTGGTAGGAGACTTACTGCCACCAAACTCTATGAGGTACAGGGTGGGCCTCC
  CCTCCCCCACTCCACATTGGCTTCAGGCTCAGTATGTTCAGTTCTGGTGAAAGATGCTGGTCCTTTTCAG
  TCTCTGTCATTAGCTAGGCTTTTCTGTTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205518 representing BC006945
  Red=Cloning site Green=Tags(s)
  
  MAVSTQQLAEELQIFGLDYEDSLLEKLAELCVLYRQTEDGMVSELIAFCTSAGKTCLTVDILNSFEYEVL
  NKKLSKAWHSASKDSGHAGTRDIVSIQELIEAEEEEETLLSSYTTPSKGPLKRVSSTPETPLTKRSVAAR
  SPRQLLSPSSFSPSATPSQKYTSRTNRGEVVTTFGSAQGLSWSGRGGSGSVSLKVVGDPEPLTGSYKAMF
  QQLMGVREVLTSKIEELGSELKEHHKIEAFTPLLVPAQEPVILLGQIGCDSNGKLNSKSVILEGDQEHSY
  GAQIPVDLSELKEYSLFPGQVVIMEGFNTTGRRLTATKLYEVQGGPPLPHSTLASGSVCSVLVKDAGPFQ
  SLSLARLFCF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC006945
• ORF Size: 1082 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC006945, AAH06945
• RefSeq Size: 2514
• RefSeq ORF: 1082
• Locus ID: 18969