Pola2 (BC006945) Mouse Tagged ORF Clone

CAT#: MR205518

Pola2 (Myc-DDK-tagged) - Mouse polymerase (DNA directed), alpha 2 (cDNA clone MGC:11533 IMAGE:3602559)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pola2
• Synonyms: AI573378
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205518 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTGTCCACCCAGCAGCTGGCGGAGGAACTACAGATCTTCGGCCTGGACTACGAGGACTCCCTGT
  TGGAGAAACTGGCAGAGCTGTGTGTTCTATACCGACAGACTGAGGACGGGATGGTCAGTGAGCTCATCGC
  CTTCTGCACCAGTGCGGGGAAGACATGCCTTACTGTAGACATCCTGAACTCTTTTGAATACGAGGTTCTG
  AACAAAAAACTCTCCAAAGCCTGGCACAGTGCCTCCAAAGACAGTGGGCATGCAGGAACCAGAGACATTG
  TTTCTATACAAGAGCTAATTGAAGCAGAGGAGGAAGAGGAGACCCTCCTAAGCTCTTATACCACACCCTC
  TAAGGGTCCTCTCAAGCGAGTTAGCAGCACCCCAGAAACCCCCCTAACCAAAAGGAGCGTCGCTGCTCGC
  AGCCCACGTCAGCTTCTCTCACCGTCAAGTTTCTCCCCGAGTGCTACCCCCTCCCAGAAATACACCTCCA
  GAACCAACCGGGGAGAAGTGGTGACCACCTTTGGCTCCGCACAGGGCTTGTCTTGGTCCGGGAGAGGAGG
  CTCTGGAAGCGTCAGTCTAAAGGTCGTGGGGGACCCAGAGCCACTCACTGGCAGCTACAAAGCGATGTTT
  CAGCAGCTCATGGGTGTTCGAGAAGTTCTGACCAGTAAGATCGAAGAGCTCGGCAGCGAGCTGAAGGAGC
  ATCACAAGATCGAGGCCTTTACCCCTCTCCTGGTCCCAGCGCAAGAGCCTGTTATTCTCCTGGGTCAGAT
  TGGCTGCGACAGCAACGGGAAGCTGAACAGCAAGTCCGTGATTCTCGAGGGGGACCAGGAGCACTCCTAT
  GGCGCGCAGATCCCAGTGGACCTATCGGAACTCAAAGAGTATTCCCTCTTCCCTGGACAGGTTGTAATTA
  TGGAAGGATTCAACACCACTGGTAGGAGACTTACTGCCACCAAACTCTATGAGGTACAGGGTGGGCCTCC
  CCTCCCCCACTCCACATTGGCTTCAGGCTCAGTATGTTCAGTTCTGGTGAAAGATGCTGGTCCTTTTCAG
  TCTCTGTCATTAGCTAGGCTTTTCTGTTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205518 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVSTQQLAEELQIFGLDYEDSLLEKLAELCVLYRQTEDGMVSELIAFCTSAGKTCLTVDILNSFEYEVL
  NKKLSKAWHSASKDSGHAGTRDIVSIQELIEAEEEEETLLSSYTTPSKGPLKRVSSTPETPLTKRSVAAR
  SPRQLLSPSSFSPSATPSQKYTSRTNRGEVVTTFGSAQGLSWSGRGGSGSVSLKVVGDPEPLTGSYKAMF
  QQLMGVREVLTSKIEELGSELKEHHKIEAFTPLLVPAQEPVILLGQIGCDSNGKLNSKSVILEGDQEHSY
  GAQIPVDLSELKEYSLFPGQVVIMEGFNTTGRRLTATKLYEVQGGPPLPHSTLASGSVCSVLVKDAGPFQ
  SLSLARLFCF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC006945
• ORF Size: 1080 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC006945, AAH06945
• RefSeq Size: 2514 bp
• RefSeq ORF: 1082 bp
• Locus ID: 18969
• MW: 38.9 kDa