Glul (BC015086) Mouse Tagged ORF Clone

CAT#: MG205788

Glul (GFP-tagged) - Mouse glutamate-ammonia ligase (glutamine synthetase) (cDNA clone MGC:14045 IMAGE:4224865)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Glul
• Synonyms: GS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205788 representing BC015086
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCTCAGCAAGTTCCCACTTGAACAAAGGCATCAAGCAAATGTACATGTCCCTGCCCCAGGGTG
  AGAAAGTCCAAGCCATGTATATCTGGGTTGATGGTACCGGAGAAGGACTGCGCTGCAAGACCCGTACCCT
  GGACTGTGAGCCCAAGTGTGTGGAAGAGTTACCTGAGTGGAACTTTGATGGCTCTAGTACCTTTCAGTCT
  GAAGGCTCCAACAGCGACATGTACCTCCATCCTGTTGCCATGTTTCGAGACCCCTTCCGCAAAGACCCCA
  ACAAGCTGGTGCTATGTGAAGTTTTCAAGTATAACCGGAAGCCTGCAGAGACCAACTTGAGGCACATCTG
  TAAACGGATAATGGACATGGTGAGCAACCAGCACCCCTGGTTTGGAATGGAGCAGGAATATACTCTTATG
  GGAACAGACGGCCACCCATTTGGTTGGCCTTCCAATGGCTTCCCTGGACCCCAAGGCCCGTATTACTGCG
  GTGTGGGAGCAGACAAGGCCTACGGCAGGGACATCGTGGAGGCTCACTACCGGGCCTGCTTGTATGCTGG
  AGTCAAGATCACGGGGACAAATGCGGAGGTTATGCCTGCCCAGTGGGAATTCCAGATAGGACCCTGTGAG
  GGGATCCGAATGGGAGATCATCTTTGGATAGCCCGTTTTATCTTGCATCGGGTGTGCGAAGACTTTGGGG
  TGATAGCAACCTTTGACCCCAAGCCCATTCCAGGGAACTGGAATGGTGCAGGCTGCCATACCAACTTCAG
  CACCAAGGCCATGCGGGAGGAGAATGGTCTGAAGTGCATTGAGGAGGCCATTGACAAACTGAGCAAGAGG
  CACCAGTACCACATCCGCGCCTACGATCCCAAGGGGGGCCTGGACAACGCCCGGCGTCTGACTGGATTCC
  ACGAAACCTCCAACATCAACGACTTTTCTGCCGGTGTTGCCAACCGCGGTGCCAGTATCCGCATTCCCCG
  GACTGTCGGCCAGGAGAAGAAGGGCTACTTTGAAGACCGTCGGCCTTCTGCCAATTGTGACCCCTATGCG
  GTGACAGAAGCCATCGTCCGCACGTGTCTCCTCAACGAAACAGGCGACGAACCCTTCCAATACAAGAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205788 representing BC015086
  Red=Cloning site Green=Tags(s)
  
  MATSASSHLNKGIKQMYMSLPQGEKVQAMYIWVDGTGEGLRCKTRTLDCEPKCVEELPEWNFDGSSTFQS
  EGSNSDMYLHPVAMFRDPFRKDPNKLVLCEVFKYNRKPAETNLRHICKRIMDMVSNQHPWFGMEQEYTLM
  GTDGHPFGWPSNGFPGPQGPYYCGVGADKAYGRDIVEAHYRACLYAGVKITGTNAEVMPAQWEFQIGPCE
  GIRMGDHLWIARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKCIEEAIDKLSKR
  HQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRGASIRIPRTVGQEKKGYFEDRRPSANCDPYA
  VTEAIVRTCLLNETGDEPFQYKN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC015086
• ORF Size: 1119 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC015086, AAH15086
• RefSeq Size: 1435
• RefSeq ORF: 1121
• Locus ID: 14645