Cyp27a1 (BC002183) Mouse Tagged ORF Clone

CAT#: MG206963

Cyp27a1 (GFP-tagged) - Mouse cytochrome P450, family 27, subfamily a, polypeptide 1 (cDNA clone MGC:7368 IMAGE:3487429)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cyp27a1
• Synonyms: 1300013A03Rik|Cyp27
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206963 representing BC002183
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGACAACCTCCTTTGGGACTTACACCAATGTGAATCTGGCTAGTGCCCCACTCTTGGAGCAAGTGA
  TGAGACAGGAGGGCAAGTACCCAATAAGAGACCACATGGATCAGTGGAAGGACCACCGAGACCACAAGGG
  CCTCACCTATGGGATCTTCATCGCACAAGGAGAGCAATGGTACCATCTGCGTCAGGCTTTGAAACAGAGG
  CTGCTGAAGCCTGACGAGGCCGCGCTCTACACGGATGCCTTAAACGAGGTTATCAGTGACTTTATCACCC
  GGCTGGACCAGGTGCGGGCAGAGAGTGAATCAGGGGACCAGGTGCCAGACATGGCTCATCTTCTCTACCA
  CCTTGCCTTGGAAGCCATCACCTATATCCTGTTTGAGAAAAGGATTGGCTGCCTGAAACCCTCCATTCCT
  GAGGACACTGCCGCCTTCATCAGATCTGTTGCAATCATGTTCCAGAACTCAGTCTATATCACTTTCCTTC
  CCAAATGGACGCGTCCTCTGCTGCCCTTTTGGAAGCGATACCTGAATGGCTGGGATAACATTTTCTCCTT
  TGGAAAGAAGCTGATTGATGAAAAAGTCCAGGAGCTAAAAGCCCAGCTACAGGAAACTGGGCCAGATGGA
  GTCCGGGTATCTGGCTACCTGCACTTCCTGCTGACCAATGAATTGCTCAGTACTCAGGAGACCATCGGCA
  CCTTTCCTGAGCTGCTTTTGGCTGGGGTGGACACGACATCCAACACACTGACATGGGCCCTGTACCACCT
  TTCAAAGAGCCCAGAGATCCAGGAGGCCTTGCACAAGGAAGTGACTGGTGTGGTGCCCTTCGGGAAGGTG
  CCCCAGCACAAGGACTTTGCCCACATGCCTCTGCTAAAAGCTGTGATTAAGGAGACCCTGCGCCTCTACC
  CTGTGGTTCCCACAAACTCCCGGATCATCACAGAAAAGGAAACTGAAATTAATGGCTTTCTCTTCCCCAA
  GAATACACAGTTTGTGTTATGCCACTACGTGGTGTCCCGGGATCCCAGTGTCTTTCCTGAGCCCAACAGC
  TTCCAGCCTCACCGATGGCTGAGGAAGAAAGAGGCTGATAACCCTGGGATCCTACATCCATTCGGCTCTG
  TGCCCTTCGGCTATGGGGTTCGGTCTTGCCTGGGTCGGAGGATTGCAGAACTGGAGATGCAACTGATGCT
  GTCAAGGCTGGTACAGAAGTATGAGATTGCCCTGGCTCCCGGGATGGGAGAAGTAAAGACTGTGTCCCGC
  ATCGTCCTGGTTCCCAGCAAGAAGGTGAGGCTGCATTTTCTGCAGAGACAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206963 representing BC002183
  Red=Cloning site Green=Tags(s)
  
  MWTTSFGTYTNVNLASAPLLEQVMRQEGKYPIRDHMDQWKDHRDHKGLTYGIFIAQGEQWYHLRQALKQR
  LLKPDEAALYTDALNEVISDFITRLDQVRAESESGDQVPDMAHLLYHLALEAITYILFEKRIGCLKPSIP
  EDTAAFIRSVAIMFQNSVYITFLPKWTRPLLPFWKRYLNGWDNIFSFGKKLIDEKVQELKAQLQETGPDG
  VRVSGYLHFLLTNELLSTQETIGTFPELLLAGVDTTSNTLTWALYHLSKSPEIQEALHKEVTGVVPFGKV
  PQHKDFAHMPLLKAVIKETLRLYPVVPTNSRIITEKETEINGFLFPKNTQFVLCHYVVSRDPSVFPEPNS
  FQPHRWLRKKEADNPGILHPFGSVPFGYGVRSCLGRRIAELEMQLMLSRLVQKYEIALAPGMGEVKTVSR
  IVLVPSKKVRLHFLQRQ
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: BC002183
• ORF Size: 1313 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC002183, AAH02183
• RefSeq Size: 1579
• RefSeq ORF: 1313
• Locus ID: 104086