Cyp27a1 (BC002183) Mouse Tagged ORF Clone

CAT#: MR206963

Cyp27a1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 27, subfamily a, polypeptide 1 (cDNA clone MGC:7368 IMAGE:3487429)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyp27a1
• Synonyms: 1300013A03Rik|Cyp27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206963 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGACAACCTCCTTTGGGACTTACACCAATGTGAATCTGGCTAGTGCCCCACTCTTGGAGCAAGTGA
  TGAGACAGGAGGGCAAGTACCCAATAAGAGACCACATGGATCAGTGGAAGGACCACCGAGACCACAAGGG
  CCTCACCTATGGGATCTTCATCGCACAAGGAGAGCAATGGTACCATCTGCGTCAGGCTTTGAAACAGAGG
  CTGCTGAAGCCTGACGAGGCCGCGCTCTACACGGATGCCTTAAACGAGGTTATCAGTGACTTTATCACCC
  GGCTGGACCAGGTGCGGGCAGAGAGTGAATCAGGGGACCAGGTGCCAGACATGGCTCATCTTCTCTACCA
  CCTTGCCTTGGAAGCCATCACCTATATCCTGTTTGAGAAAAGGATTGGCTGCCTGAAACCCTCCATTCCT
  GAGGACACTGCCGCCTTCATCAGATCTGTTGCAATCATGTTCCAGAACTCAGTCTATATCACTTTCCTTC
  CCAAATGGACGCGTCCTCTGCTGCCCTTTTGGAAGCGATACCTGAATGGCTGGGATAACATTTTCTCCTT
  TGGAAAGAAGCTGATTGATGAAAAAGTCCAGGAGCTAAAAGCCCAGCTACAGGAAACTGGGCCAGATGGA
  GTCCGGGTATCTGGCTACCTGCACTTCCTGCTGACCAATGAATTGCTCAGTACTCAGGAGACCATCGGCA
  CCTTTCCTGAGCTGCTTTTGGCTGGGGTGGACACGACATCCAACACACTGACATGGGCCCTGTACCACCT
  TTCAAAGAGCCCAGAGATCCAGGAGGCCTTGCACAAGGAAGTGACTGGTGTGGTGCCCTTCGGGAAGGTG
  CCCCAGCACAAGGACTTTGCCCACATGCCTCTGCTAAAAGCTGTGATTAAGGAGACCCTGCGCCTCTACC
  CTGTGGTTCCCACAAACTCCCGGATCATCACAGAAAAGGAAACTGAAATTAATGGCTTTCTCTTCCCCAA
  GAATACACAGTTTGTGTTATGCCACTACGTGGTGTCCCGGGATCCCAGTGTCTTTCCTGAGCCCAACAGC
  TTCCAGCCTCACCGATGGCTGAGGAAGAAAGAGGCTGATAACCCTGGGATCCTACATCCATTCGGCTCTG
  TGCCCTTCGGCTATGGGGTTCGGTCTTGCCTGGGTCGGAGGATTGCAGAACTGGAGATGCAACTGATGCT
  GTCAAGGCTGGTACAGAAGTATGAGATTGCCCTGGCTCCCGGGATGGGAGAAGTAAAGACTGTGTCCCGC
  ATCGTCCTGGTTCCCAGCAAGAAGGTGAGGCTGCATTTTCTGCAGAGACAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206963 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWTTSFGTYTNVNLASAPLLEQVMRQEGKYPIRDHMDQWKDHRDHKGLTYGIFIAQGEQWYHLRQALKQR
  LLKPDEAALYTDALNEVISDFITRLDQVRAESESGDQVPDMAHLLYHLALEAITYILFEKRIGCLKPSIP
  EDTAAFIRSVAIMFQNSVYITFLPKWTRPLLPFWKRYLNGWDNIFSFGKKLIDEKVQELKAQLQETGPDG
  VRVSGYLHFLLTNELLSTQETIGTFPELLLAGVDTTSNTLTWALYHLSKSPEIQEALHKEVTGVVPFGKV
  PQHKDFAHMPLLKAVIKETLRLYPVVPTNSRIITEKETEINGFLFPKNTQFVLCHYVVSRDPSVFPEPNS
  FQPHRWLRKKEADNPGILHPFGSVPFGYGVRSCLGRRIAELEMQLMLSRLVQKYEIALAPGMGEVKTVSR
  IVLVPSKKVRLHFLQRQ
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: BC002183
• ORF Size: 1311 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC002183, AAH02183
• RefSeq Size: 1579 bp
• RefSeq ORF: 1313 bp
• Locus ID: 104086
• MW: 50.2 kDa