Tubb4b (NM_146116) Mouse Tagged ORF Clone

CAT#: MG207096

Tubb4b (GFP-tagged) - Mouse tubulin, beta 2c (Tubb2c)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tubb4b
• Synonyms: 4930542G03Rik; Tubb2c; Tubb2c1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207096 representing NM_146116
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGAGATCGTGCACCTGCAGGCTGGGCAGTGCGGCAACCAGATTGGCGCCAAGTTCTGGGAGGTAA
  TCAGCGACGAGCATGGCATTGATCCCACTGGCACTTACCACGGAGATAGCGACCTCCAGCTGGAGCGCAT
  CAACGTGTACTACAACGAAGCCACCGGTGGCAAGTATGTGCCCCGCGCCGTGCTCGTGGACTTGGAGCCC
  GGCACCATGGACTCGGTGCGTTCAGGGCCCTTCGGGCAGATCTTCAGACCTGATAACTTCGTCTTTGGTC
  AGAGTGGGGCTGGGAACAACTGGGCCAAGGGGCACTACACAGAAGGTGCAGAGCTGGTTGACTCGGTGTT
  GGACGTTGTGAGAAAGGAAGCTGAGAGCTGTGACTGCCTGCAGGGCTTCCAGCTGACCCACTCCCTGGGT
  GGGGGGACTGGGTCTGGGATGGGTACCCTCCTTATCAGCAAGATCCGGGAGGAGTACCCAGACAGAATCA
  TGAACACCTTCAGTGTGGTACCTTCCCCCAAGGTGTCGGACACAGTGGTTGAGCCCTACAATGCCACCCT
  TTCAGTCCACCAGCTGGTTGAAAACACAGATGAGACCTATTGTATTGATAATGAAGCACTTTATGACATC
  TGCTTCAGAACCCTAAAGCTGACCACACCCACTTACGGTGACCTGAACCATCTAGTGTCCGCCACCATGA
  GTGGGGTAACCACTTGCCTGCGATTCCCTGGCCAGCTAAATGCTGACCTGCGGAAACTGGCTGTAAATAT
  GGTGCCCTTCCCTCGCCTGCACTTCTTCATGCCTGGCTTTGCCCCCTTGACCAGCCGGGGCAGCCAGCAG
  TACCGTGCCCTGACAGTTCCTGAGCTCACCCAGCAGATGTTTGATGCCAAGAACATGATGGCTGCCTGTG
  ATCCAAGACATGGGCGCTACTTGACTGTGGCTGCCGTGTTCAGGGGCCGCATGTCTATGAAGGAGGTGGA
  CGAACAGATGCTTAATGTCCAAAACAAGAACAGCAGCTACTTTGTTGAGTGGATCCCCAACAATGTGAAG
  ACAGCTGTCTGTGACATTCCACCTCGGGGCCTGAAAATGTCGGCCACCTTCATTGGCAACAGCACCGCTA
  TTCAGGAGCTGTTCAAACGCATCTCAGAGCAGTTCACAGCCATGTTCCGACGCAAGGCCTTCCTACACTG
  GTACACGGGTGAAGGCATGGATGAGATGGAGTTCACTGAGGCTGAGAGCAACATGAACGACCTGGTGTCC
  GAGTACCAGCAGTACCAGGACGCTACAGCTGAGGAAGAGGGAGAGTTTGAGGAGGAGGCTGAGGAGGAGG
  TGGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207096 representing NM_146116
  Red=Cloning site Green=Tags(s)
  
  MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEP
  GTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLG
  GGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDI
  CFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQ
  YRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK
  TAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
  EYQQYQDATAEEEGEFEEEAEEEVA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146116
• ORF Size: 1335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_146116.1, NP_666228.1
• RefSeq Size: 1561
• RefSeq ORF: 1338
• Locus ID: 227613
• Gene Summary: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProtKB/Swiss-Prot Function]