Tubb4b (NM_146116) Mouse Tagged ORF Clone

CAT#: MR207096

Tubb4b (Myc-DDK-tagged) - Mouse tubulin, beta 2C (Tubb2c)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tubb4b
• Synonyms: 4930542G03Rik; Tubb2c; Tubb2c1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207096 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGAGATCGTGCACCTGCAGGCTGGGCAGTGCGGCAACCAGATTGGCGCCAAGTTCTGGGAGGTAA
  TCAGCGACGAGCATGGCATTGATCCCACTGGCACTTACCACGGAGATAGCGACCTCCAGCTGGAGCGCAT
  CAACGTGTACTACAACGAAGCCACCGGTGGCAAGTATGTGCCCCGCGCCGTGCTCGTGGACTTGGAGCCC
  GGCACCATGGACTCGGTGCGTTCAGGGCCCTTCGGGCAGATCTTCAGACCTGATAACTTCGTCTTTGGTC
  AGAGTGGGGCTGGGAACAACTGGGCCAAGGGGCACTACACAGAAGGTGCAGAGCTGGTTGACTCGGTGTT
  GGACGTTGTGAGAAAGGAAGCTGAGAGCTGTGACTGCCTGCAGGGCTTCCAGCTGACCCACTCCCTGGGT
  GGGGGGACTGGGTCTGGGATGGGTACCCTCCTTATCAGCAAGATCCGGGAGGAGTACCCAGACAGAATCA
  TGAACACCTTCAGTGTGGTACCTTCCCCCAAGGTGTCGGACACAGTGGTTGAGCCCTACAATGCCACCCT
  TTCAGTCCACCAGCTGGTTGAAAACACAGATGAGACCTATTGTATTGATAATGAAGCACTTTATGACATC
  TGCTTCAGAACCCTAAAGCTGACCACACCCACTTACGGTGACCTGAACCATCTAGTGTCCGCCACCATGA
  GTGGGGTAACCACTTGCCTGCGATTCCCTGGCCAGCTAAATGCTGACCTGCGGAAACTGGCTGTAAATAT
  GGTGCCCTTCCCTCGCCTGCACTTCTTCATGCCTGGCTTTGCCCCCTTGACCAGCCGGGGCAGCCAGCAG
  TACCGTGCCCTGACAGTTCCTGAGCTCACCCAGCAGATGTTTGATGCCAAGAACATGATGGCTGCCTGTG
  ATCCAAGACATGGGCGCTACTTGACTGTGGCTGCCGTGTTCAGGGGCCGCATGTCTATGAAGGAGGTGGA
  CGAACAGATGCTTAATGTCCAAAACAAGAACAGCAGCTACTTTGTTGAGTGGATCCCCAACAATGTGAAG
  ACAGCTGTCTGTGACATTCCACCTCGGGGCCTGAAAATGTCGGCCACCTTCATTGGCAACAGCACCGCTA
  TTCAGGAGCTGTTCAAACGCATCTCAGAGCAGTTCACAGCCATGTTCCGACGCAAGGCCTTCCTACACTG
  GTACACGGGTGAAGGCATGGATGAGATGGAGTTCACTGAGGCTGAGAGCAACATGAACGACCTGGTGTCC
  GAGTACCAGCAGTACCAGGACGCTACAGCTGAGGAAGAGGGAGAGTTTGAGGAGGAGGCTGAGGAGGAGG
  TGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207096 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEP
  GTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLG
  GGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDI
  CFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQ
  YRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK
  TAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
  EYQQYQDATAEEEGEFEEEAEEEVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146116
• ORF Size: 1338 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146116.1, NM_146116.2, NP_666228.1
• RefSeq Size: 1600 bp
• RefSeq ORF: 1338 bp
• Locus ID: 227613
• MW: 49.8 kDa
• Gene Summary: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProtKB/Swiss-Prot Function]