Chrnb3 (BC058193) Mouse Tagged ORF Clone

CAT#: MG207420

Chrnb3 (GFP-tagged) - Mouse cholinergic receptor, nicotinic, beta polypeptide 3 (cDNA clone MGC:67663 IMAGE:5357762)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Chrnb3
• Synonyms: 5730417K16Rik|Acrb3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207420 representing BC058193
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGGCTTCCTACGGGTCTTCTTGGCTCTCAGTGCCACTCTCTCAGGTTCCTGGGTGACTCTCACAG
  CCACTGCGGGACTCAGCTCAGTGGCTGAACATGAAGACGCACTCCTCAGACATTTGTTCCAAGGTTATCA
  GAAATGTGTCCGCCCTGTGTTGAATTCCAGTGACATCATAAAAGTGTATTTTGGATTAAAGATATCTCAG
  CTTGTAGATGTGGATGAAAAGAATCAGCTGATGACAACAAACGTGTGGCTGAAGCAGGAATGGACAGACC
  AAAAATTACGATGGAATCCCGAAGACTATGGTGGAATTAATTCGATAAAGGTTCCATCGGAGTCGCTCTG
  GCTGCCTGACATAGTTCTCTTTGAAAATGCTGACGGACGTTTTGAGGGCTCTCTCATGACCAAGGCCATT
  GTGAAATCCAGCGGAACCGTCAGTTGGACTCCTCCCGCCAGCTACAAAAGTTCCTGTACCATGGATGTCA
  CATTTTTCCCGTTCGATAAGCAGAACTGCTCAATGAAGTTCGGATCCTGGACTTACGATGGTACCATGGT
  CGACCTCATTCTGATCAATGAAAACGTTGACCGGAAAGACTTTTTTGATAACGGAGAGTGGGAGATACTA
  AATGCAAAGGGGATGAAGGGCAACAGAAGAGAAGGCTTTTACTCCTATCCGTTTGTTACCTACTCTTTTG
  TTCTGAGACGCCTGCCCCTGTTTTACACCCTCTTTTTGATAATCCCCTGCCTGGGGCTGTCTTTTCTCAC
  GGTCCTGGTGTTCTACCTACCCTCCGACGAAGGGGAAAAACTCTCCTTATCCACCTCGGTTTTGGTTTCT
  TTGACAGTGTTTCTTTTAGTAATCGAAGAAATAATCCCGTCTTCTTCAAAAGTCATCCCTCTCATTGGGG
  AGTACCTCCTCTTCATTATGATATTTGTCACGCTGTCTATTATTGTCACGGTTTTTGTAATTAATGTCCA
  CCACAGATCTTCCTCAACGTACCATCCCATGGCCCCCTGGGTGAAGAGGCTGTTTCTGGAAAAACTCCCG
  AGATGGCTTTGCATGAAGGACCCCAGGGACCGCTTCTCTTTCCCCGATGGAACGGAGAGTAAGGGAACCG
  TGAGGGGGAAATTTCCAGGGAAAAAGAAGCAGACTCCTACCAGTGATGGAGAAAGAGTTCTGGTCGCTTT
  CCTGGAGAAGGCCTCTGAGTCCATCAGATACATCTCGAGGCATGTGAAGAAGGAACACTTCATCAGTCAG
  GTTGTTCAAGACTGGAAATTCGTGGCTCAGGTTCTTGACCGCATCTTCCTCTGGCTCTTTCTGACAGCTT
  CGGTTTTGGGCTCTGTCCTGATTTTTATTCCAGCCTTGAAGATGTGGATACATCGTTTCCAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207420 representing BC058193
  Red=Cloning site Green=Tags(s)
  
  MTGFLRVFLALSATLSGSWVTLTATAGLSSVAEHEDALLRHLFQGYQKCVRPVLNSSDIIKVYFGLKISQ
  LVDVDEKNQLMTTNVWLKQEWTDQKLRWNPEDYGGINSIKVPSESLWLPDIVLFENADGRFEGSLMTKAI
  VKSSGTVSWTPPASYKSSCTMDVTFFPFDKQNCSMKFGSWTYDGTMVDLILINENVDRKDFFDNGEWEIL
  NAKGMKGNRREGFYSYPFVTYSFVLRRLPLFYTLFLIIPCLGLSFLTVLVFYLPSDEGEKLSLSTSVLVS
  LTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIIVTVFVINVHHRSSSTYHPMAPWVKRLFLEKLP
  RWLCMKDPRDRFSFPDGTESKGTVRGKFPGKKKQTPTSDGERVLVAFLEKASESIRYISRHVKKEHFISQ
  VVQDWKFVAQVLDRIFLWLFLTASVLGSVLIFIPALKMWIHRFH
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC058193
• ORF Size: 1394 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC058193, AAH58193
• RefSeq Size: 4454
• RefSeq ORF: 1394
• Locus ID: 108043