Polr1e (BC034783) Mouse Tagged ORF Clone

CAT#: MG207725

Polr1e (GFP-tagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Polr1e
• Synonyms: 53kDa
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207725 representing BC034783
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCCTGGAGTCTCCTGGGATGGATGACCAGGCTGGAGACACGGAGACAGAGGCGCTACAGAGTG
  CAAGGTGGCTGTACTGTGGAGAGCCCGACGACAGGCAGAAAGCTGTGCTGGTCCAGTTCTCCAATGGGAA
  GCTGCAGAATCCGGGCGACATGCGCTTCACCTTGTACAACAGCACCGACTTAGTAAACCCCAGGCAGAGG
  AGTCACCGGATCGTGGCAGCTGAAACAGACAGGCTCTCCTATGTCGGAAACAATTTTGGGACCGGTGCCC
  TCAAATGCAATGCTCTTTGCAGACACTTTGTGGGAATTCTGAATAAGACCTCTGGCCAGATGGAAGTCTA
  TGATGCCGAACTGTTCAACATGCAGCCGCTGTTTGCTGGAATGGGAACTGAAGTCATCAAACTTGGTGGC
  CAACACCTTTACCTGCTGGCCTTCTGCCAACCCTCCAAGAACCTGGCTGAGGCAGGTGACCTGCTGCTGA
  GCCGACACCGGCAAGGCCATTGCATTGCAGTCCTGCTGGATGACGATGCTATTGAGCGAGAGCCTCCTCT
  GGAGAATCAGAACAAGACTTTCAGGGACAAGTTGGATTCTTGCATTGAAGCCTTTGGAAGCACCAAGCAG
  AAGCGGTCCCTAAACTCCAGGCGGATGAACAAAGTTGGCAGCGAGTCTCTGAATCTCAGTGTGGCGAAAG
  CCGCGGAGAGTATCATTGATACAAAGGGTGTGAATGCGCTGGTCAGTGATGCCATGCAGGATGACTTACA
  ATATGACGCCCTCTACCTTCCTCCCTGCTACGCTGACGCAGCCAAGCCGGAAGACGTGTATAGATTTGAA
  GACATTCTGTCCCCGGCCGAGTATGATGCTCTTGAGAGCCCATCCGAAGCCTTCAGAAAGGTCACATCGG
  AAGACATACTGAAGATGATTGAAGAGAACAGCCACTGCTCCTACGTCATAGAAATGCTGAAGTCTCTGCC
  CATAGATGAGGTCCACCGGAACCGGCAAGCCCGGAGCATCTGGTTCCTAGACGCCCTCATCAGATTCCGA
  GCTCAGAAAGTGATAAAGGGGAAAAGAGCCTTAGGGCCTGGAATCCCGCACATTATCAACACCAAGCTGC
  TGAAACAGTTCACCTGCTTGACCTACAACAACGGCAGGTTACAGAACCTAATTTCAAGTTCTATGAGGGC
  CAAGATCACTTCGTATGCGATCATACTTGCCCTGCACATAAATAACTTCCAGGTTGACCTGACAGCGTTG
  CAGAAGGACTTGAAGCTGAGTGAGAAAAGGATTATTGAGATAGCGAAAGCCATGAGGCTGAAGATCTCCA
  AACAAAAGGTGTCCCTGGCAGATGGCAGGGAAGAGAGTCACAGACTGGGCACTCTGTCTGTCCCACTGCC
  TCCAGCCCAGAACTCTGATCGACAGTCAAAGCGGAGGAAAATGAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207725 representing BC034783
  Red=Cloning site Green=Tags(s)
  
  MAALESPGMDDQAGDTETEALQSARWLYCGEPDDRQKAVLVQFSNGKLQNPGDMRFTLYNSTDLVNPRQR
  SHRIVAAETDRLSYVGNNFGTGALKCNALCRHFVGILNKTSGQMEVYDAELFNMQPLFAGMGTEVIKLGG
  QHLYLLAFCQPSKNLAEAGDLLLSRHRQGHCIAVLLDDDAIEREPPLENQNKTFRDKLDSCIEAFGSTKQ
  KRSLNSRRMNKVGSESLNLSVAKAAESIIDTKGVNALVSDAMQDDLQYDALYLPPCYADAAKPEDVYRFE
  DILSPAEYDALESPSEAFRKVTSEDILKMIEENSHCSYVIEMLKSLPIDEVHRNRQARSIWFLDALIRFR
  AQKVIKGKRALGPGIPHIINTKLLKQFTCLTYNNGRLQNLISSSMRAKITSYAIILALHINNFQVDLTAL
  QKDLKLSEKRIIEIAKAMRLKISKQKVSLADGREESHRLGTLSVPLPPAQNSDRQSKRRKMN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC034783
• ORF Size: 1448 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC034783, AAH34783
• RefSeq Size: 1757
• RefSeq ORF: 1448
• Locus ID: 64424